摘要
目的比较黄连-吴茱萸药对配方颗粒、单煎与共煎体外抑制幽门螺杆菌(Helicobacter pylori,H.pylori)的作用,探讨药对配方颗粒与传统煎煮的等效性。方法采用麦氏比浊仪测定细菌浓度,绘制细菌生长曲线;采用琼脂稀释法制备含药血平板,测定黄连-吴茱萸不同配比(6∶1、5∶2、4∶3、3∶4、2∶5、1∶6)的配方颗粒剂和共煎液、黄连配方颗粒剂、吴茱萸配方颗粒剂、黄连单煎液及吴茱萸单煎液对H.pylori的最小抑菌浓度(MIC)。结果分别作用H.pylori 48 h后,琼脂稀释法测得黄连颗粒剂对Hp SS1的MIC是0.900 mg·mL^(-1),单煎液为0.869 mg·mL^(-1);吴茱萸配方颗粒剂对Hp SS1的MIC为1.140mg·mL^(-1),单煎液为1.118 mg·mL^(-1);黄连、吴茱萸配方颗粒剂与单煎(传统煎煮)对H.pylori生长的抑制作用差异没有统计学意义(P> 0.05);黄连∶吴茱萸=6∶1的配方颗粒剂对Hp SS1的MIC为0.810mg·mL^(-1),相应共煎液的为0.396 mg·mL^(-1);1∶6配方颗粒剂的MIC为1.800 mg·mL^(-1),相应共煎液的为0.818 mg·mL^(-1),提示黄连、吴茱萸不同配比的配方颗粒剂与共煎液中,黄连∶吴茱萸=6∶1对H.pylori的抑制作用最强,且共煎液抑制H.pylori的作用明显优于相应的配方颗粒剂,差异有统计学意义(P <0.01)。结论黄连-吴茱萸不同配比颗粒剂、单煎液和共煎液均能有效抑制H.pylori的生长,黄连∶吴茱萸=6∶1的抑制作用最强,共煎液抑制作用明显优于相应配比的颗粒剂,本实验为配方颗粒剂与共煎的等效性研究和临床应用提供了一定的实验依据。
Objective To compare the inhibitory effect of formula granule, single and mixed decoction of Huanglian and Wuzhuyu at different proportions on Helicobacter pylori(H.pylori) and the equivalence of formula granule and traditional decocting. Methods The concentration of bacteria was determined by Maxwell Turbidimetry(MAE), and the growth curve of bacteria was obtained. The minimum inhibition concentration(MIC) of formula granule and traditional decoction of Huanglian, and Wuzhuyu at different proportions(6∶1, 5∶2, 4∶3, 3∶4, 2∶5, and 1∶6) was measured by agar dilution method. Results After the bacteria were treated with of formula granules and traditional decoctions for 48 h respectively, Hp SS1 was taken as example, the MIC was confirmed as 0.900 mg·mL^-1 for Huanglian granules and 0.869 mg·mL^-1 for its traditional decoction, while 1.140 mg·mL^-1 for Wuzhuyu granules and 1.118 mg·mL^-1 for its traditional decoction, with no difference between the granules and their decoctions(P > 0.05). The MIC was 0.810 mg·mL^-1 for granules of Huanglian and Wuzhuyu at 6∶1 and 0.396 mg·mL^-1 for granules of Huanglian and Wuzhuyu at 1∶6, 1.800 mg·mL^-1 and 0.818 mg·mL^-1 for their corresponding decoctions. The inhibition of Huanglian and Wuzhuyu at 6∶1 was the strongest, regardless of the formula granules or traditional decoctions, and the effects of traditional decoctions outweighed their formula granules(P<0.01). Conclusion H.pylori can be effectively inhibited by formula granule, single and mixed decoction of Huanglian and Wuzhuyu at different proportions. Proportions at 6∶1 has the best compatibility. and traditional decoctions have better effect than their formula granules. This experiment provides a experimental basis for the equivalence and clinical application between formula granule and decoction.
作者
周红祖
陈永恒
邱裕豪
侯梅轩
沈茂洽
赵本科
余惠旻
ZHOU Hong-zu;CHEN Yong-heng;QIU Yu-hao;HOU Mei-xuan;SHEN Mao-qia;ZHAO Ben-ke;YU Hui-min(Shenzhen Hospital of Chinese Medicine,Shenzhen Guangdong 518020;School of Medicine,Shenzhen University,Shenzhen Guangdong 518060)
出处
《中南药学》
CAS
2019年第1期48-53,共6页
Central South Pharmacy
基金
深圳市知识创新基础研究(自由探索)(No.JCYJ20170818094033689)
深圳大学教学改革项目(No.JG2017035)
深圳大学聚徒+创研项目(No.2018延续)
中药特色技术传承人才培训项目(粤中医办函[2016]54号)
关键词
黄连
吴茱萸
配方颗粒剂
单煎
共煎
幽门螺杆菌
Huanglian
Wuzhuyu
formula granule
single decoction
mixed decoction
Helicobacterpylori
