摘要
到目前为止 ,原核注射是最可靠 ,也是使用最广泛的动物转基因方法 .但该方法存在整合效率太低及不能定点整合的问题 .在过去的 2 0年里 ,出现了一些新的转基因方法 ,包括精子介导、反转录病毒介导、携带外源基因体细胞的核移植、ES细胞基因打靶技术等 .但这些方法都未能根本地解决存在的问题 .最近的一些文献中报道转基因技术在原有方法的基础上做出了改进后 ,取得了突破性进展 .
The technique of pronuclear microinjcetion is the most reliable and popular method for transgenic livestock production to date. However, pronuclear microinjection has been proven to be inefficient in producing transgenics and in inserting a piece of DNA into a specific site in the host genome. In the past 20 years, some new approaches have been used. These include sperm mediated DNA transfer, retroviral mediated DNA transfer into oocytes, somatic cell carrying exogenous genes nuclear transfer, the use of embryonic stem(ES) cell. But these ways can not solve existing problems thoroughly. In recent papers, the existing techniques have been improved and significant progress has been made in developing transgenic techniques.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2000年第2期124-126,共3页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金!资助项目 ( 3 9770 0 98)
