摘要
根据Genebank提供的大鼠HO-1(Hmoxl,血红素加氧酶-1)基因序列,参照RNA干扰序列设计原则,设计针对HO-1的4个RNA干扰序列,定向克隆至表达载体pGPU6-GFP-Neo中,最后采用BamH I和Pst I酶切凝胶电泳鉴定。鉴定结果显示成功合成了荷载HO-1-shRNA的表达载体。
Four HO-1 RNA interference sequences were designed according to HO-1（Hmoxl） gene sequences of rats from Genebank with reference to the designing principle of RNA interfering sequences, and were cloned into pGPU6-GFP-Neo expression vector, then were identified by BamH I and Pst 1 enzyme gel electrophoresis finally. It was proved that we successfully synthesis the expression vector loading HO-1-shRNA.
出处
《广东化工》
CAS
2011年第7期21-21,27,共2页
Guangdong Chemical Industry
基金
国家自然科学基金项目(30800446
30800309)
江苏省高校自然科学基金项目(09KJB310016)
江苏省"青蓝工程"项目资助
