摘要
RNAi与高内涵筛选联合应用,可作为药物研发过程中大规模靶标筛选、候选靶标确证的重要方法,还可用于化合物的筛选及作用机制研究;在HCS技术平台上平行进行功能基因组学和化学遗传学研究可同时筛选靶标和先导化合物。RNAi与HCS联合应用在药物研发中显示显著优势的同时,也常被数据的捕获、管理、分析等技术挑战所限制,尤其是海量图像的自动分析,如细胞分界及细胞表型识别分类等。
Combination of RNAi with high content screening （HCS） provides a powerful tool to carry out large-scale screens to discover and validate drug targets. It is also very useful in compound screening and mechanism research. Parallel use of chemical genetic approach and genome-wide RNAi screening on HCS platform can facilitate identifying drug targets and discovering leading compounds at the same time. The application of RNAi combined with HCS shows significant advantages in drug development. However, it is still often limited by technical challenges in the processes of acquisition, management and analysis of data, particularly in automatic analysis for massive images, such as cellular segmentation and cellular phenotype classification.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2009年第8期681-685,共5页
Chinese Journal of New Drugs
关键词
RNA干扰
高内涵筛选
靶标筛选
化合物筛选
图像分析
RNAi
high content screening
target identification
compound screening
image analysis
