摘要
目的:通过研究腺嘌呤对醛固酮合成酶途径的影响,探讨其导致"肾虚多尿"的机制所在。方法:利用酶联免疫分析法分别检测正常组、腺嘌呤组动物血中皮质酮(Cort)、醛固酮(ALD)的含量;并采用RT-PCR的方法检测各组动物肾脏CYP11B2 mRNA的表达。结果:与正常组比较,腺嘌呤可明显减少大鼠血中Cort,ALD含量,并下调模型大鼠肾脏CYP11B2mRNA的表达。结论:腺嘌呤肾虚多尿大鼠血中Cort,ALD含量减少,肾脏CYP11B2 mRNA的表达下调,影响醛固酮合成酶途径,从而减少ALD的合成,导致水液代谢异常引发多尿,本实验研究结果从醛固酮合成酶的角度阐明了腺嘌呤导致"肾虚多尿"的作用机制。
Objective：To explore the mechanism for kidney deficiency and diuresis by studying the action of adenine to ALD synthase.Methods：The contents of Cort,ALD in blood were determined and the mRNA expression of CYP11B2 was evaluated with ELISA and RT-PCR in model group and control group.Results：Compared with control group,adenine could remarkedly decrease the contents of Cort,ALD in rat blood;and downregulate the mRNA expression of CYP11B2.Conclusion：Adenine can decrease the contents of Cort,ALD in blood and downregulate the mRNA expression of CYP11B2 in rats.It can decrease the synthesis of ALD by adjusting the ALD synthase.The results may expound the mechanism of kidney deficiency and diuresis induced by adenine from the angle of ALD synthase approach.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第2期64-67,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金(30873425)
关键词
腺嘌呤
肾虚多尿
醛固酮合成酶
adenine
kidney deficiency and diuresis
ALD synthase
