摘要
根据水稻抗逆相关锌指蛋白基因的保守区序列设计引物,以毛竹基因组DNA和cDNA为模板,采用PCR方法,成功扩增出1个含有完整阅读框架的cDNA序列,长度为495bp,序列无内含子,共编码164个氨基酸,将其命名为PeZFP基因(GenBank登录号:FJ472953)。氨基酸序列(GenBank登录号:ACL01101)的分析结果表明,PeZFP与其他锌指结构蛋白有较高的同源性,同水稻锌指结构抗逆蛋白OSIAP1序列相似性高达87.7%,且其序列C端具有典型的AN1类型的锌指结构Cx2-4Cx9-12Cx2Cx4Cx2Hx5HxC,在N端具有典型的A20类型锌指蛋白结构,推测此PeZFP为植物抗逆OsIAP1类似基因,在功能上与毛竹抗逆性相关。
A zinc-finger protein gene from phyllostachys edulis, named as PeZFP （ GenBank accession number： FJ472953 ） , was cloned through PCR using primers designed according to the zinc-finger protein genes conserved region of other plant. The coding region of the genomie clone of PeZFP is continuous without intron. The eDNA of PeZFP gene contains an open reading frame of 495 bp and codes for a protein of 164 aa（ GenBank accession number ACL01101 ）. The database search using the amino acid sequence as query showed high homology to several zinc-finger proteins,especially the PeZFP sequence showed the maximum homology（87.7% identity） to OSISAP1 gene （Oryza sativa subspecies indica stress-associated protein gene ）, and it has a typically conserved sequence Cx2-4Cx9- 12Cx2Cx4Cx2Hx5HxC（ X represents any amino acids）at the C-terminal AN1-type zinc finger and the N-terminal A20 type zinc- finger. Therefore, it predicted that the PeZFP could be an important determinant of stress response in bamboo.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第1期87-92,共6页
Biotechnology Bulletin
基金
国家"863"计划项目(2007AA021403)
浙江省自然科学基金资助项目(Y307469)
浙江省现代森林培育技术重点实验室开放基金
关键词
毛竹
锌指蛋白
克隆
Phyllostachys edulis Zinc-finger protein Cloning
