摘要
目的探讨藤茶总黄酮的体外抗肿瘤作用。方法采用MTT法、生长曲线法、细胞集落形成法观察藤茶总黄酮对人胃癌SGC-7901细胞抑制作用。结果藤茶总黄酮能明显抑制体外培养的SGC-7901细胞的生长,MTT法IC50为20.71μg.ml-1;细胞生长曲线法提示其对SGC-7901细胞的生长也有明显的抑制作用;集落形成法,当药物浓度在9.90μg.ml-1以上时抑制率为100%,当药物浓度为6.60μg.ml-1时抑制率为69.80%,当药物浓度为4.40μg.ml-1时抑制率为30.23%。结论藤茶总黄酮对体外SGC-7901细胞的生长有明显的抑制作用。
Objective To investigate the anti - tumor effect of Vine tea extract in vitro. Methods The growth inhibition was analyzed by MTT, cell colony and cell growth curve measurement in the stomach cancer cell SGC -7901. Results Vine tea extract could obviously inhibit the growth of euhured SGC-7901 ceils, the IC50 in MTT assay was 20.71 μg · ml^-1; Vine tea extract could obviously affect the tumor cell growth. The inhibitory, ratio in cell colony assay was 100 % when medicine density was over 9.90 μg · ml^-1, the repressive ratio in cell colony assay was 69.80 % when medicine density was 6.60μg · ml^-1 ;the repressive ratio in cell colony assay was 30.23 % when medicine density was 4.40 μg · ml^-1 . Conclusion Vine tea extract can inhibit the growth of SGC - 7901 in vitro.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第5期1158-1159,共2页
Lishizhen Medicine and Materia Medica Research
基金
广西科学基金资助项目(No.桂科基0639052)
