摘要
目的检测nucleostemin(NS)基因在胃癌组织和胃癌SGC-7901细胞中的表达情况。方法21例胃癌手术标本,提取胃癌组织和胃癌SGC-7901细胞株总RNA,用半定量RT-PCR方法检测胃癌组织和SGC-7901中NS基因的表达情况。用脂质体法将PCDNA4/C-NS-silencer质粒及空载体PCDNA4/C-vector质粒分别转染SGC-7901细胞,分别命名为silencer组和vector组,未转染的SGC-7901细胞记为normal组,RT-PCR方法检测NS基因表达量。用MTT法检测3组细胞的生长增殖情况。结果胃癌组织和SGC-7901细胞中皆有NS基因表达。定性分析显示,与vector组和normal组比较,silencer组细胞趋于分化,NS基因表达量下降。细胞培养24 h、48 h、72 h后,si-lencer组和vector组细胞增殖抑制率间差别均有统计学意义(P<0.01)。结论胃癌组织和SGC-7901细胞中有NS基因表达;NS基因特异性RNA干扰使NS基因表达量下降,抑制SGC-7901细胞株体外增殖。
Objective To detect the expression of nucleostemin （NS） gene in gastric cancer （GC） tissues and SGC -7901 cells, and the effect of NS on GC tissues and cell proliferation （CP）. Methods Total RNA was isolated from GC tissues and SGC -7901 cell strains, NS gene expressions in GC tissues and SGC -7901 cells were determined by RT -PCR; By liposome transfection law, SGC -7901 cells were transfected with PCDNA4/C -NS -silencer plasmid or no -load carrier PCD- NA4/C -vector plasmid, which were named as silencer group and vector group, respectively, and the non - tranfected SGC - 7901 cells were named as normal group. NS gene expressions were determined by RT - PCR and the celt growth and proliferation conditions of the three groups were detected by MTT. Results NS gene expressions were found in both GC tissues and SGC - 7901 cells; qualitative analysis showed that in silencer group the cells became more differentiated and the level of NS gene expression reduced as compared with vector group and normal group. The cell proliferation inhibitory rates were above 60% in silencer group and vector group at 24 h, 48, 72 h after cell culture （ P 〈 0. 01 ）. Conclusion NS gene is expressed in GC tissues and SGC -7901 cells; interference of NS gene specific RNA reduces the level of NS gene expression and significantly inhibits GC tissue and SGC -7901 cell proliferation.
出处
《中国全科医学》
CAS
CSCD
2008年第16期1461-1463,共3页
Chinese General Practice
