摘要
目的:满足水产中对中性蛋白酶的需求。方法:以实验室保藏的米曲霉ZW为出发菌株,经Co60定向诱变,通过透明圈法初筛、摇瓶发酵复筛,筛选中性蛋白酶活力高的菌株,并对其进行产酶酶系分析。结果:筛选到的米曲霉ZW-06产中性蛋白酶酶活可达15000U/g干曲,比诱变前酶活提高了74%,是目前国内报道的固体发酵产中性蛋白酶活力最高的菌株;经过10代传代之后,酶活力仍保持稳定。通过对米曲霉ZW-06进行产酶酶系分析,发现发酵产物中除了有较高的中性蛋白酶酶活,还有较高的木聚糖酶和酸性纤维素酶酶活,酶活分别达到49879U/g干曲和21099U/g干曲。
Objective：To satisfy the need of neutral proteinase in feed industry.Methods：A neutral protease high-producing strain Aspergillus oryzae ZW-06 was separated from Aspergillus oryzae ZW by Co60 treatment.Results：The neutral proteinase enzyme activity of Aspergillus oryzae ZW-06 was 15 000U/g and was increased 74% than control.The producing condition of Aspergillus oryzae ZW-06 was stable after being transfered 10 generations.Enzyme kinds analysis showed that Aspergillus oryzae ZW-06 produced not only neutral proteinase but also xylase and acidic cellulose, and enzyme aetlvity reached 49 879U/g and 21 099U/g separately. Conclusion： Aspergillus oryzae ZW-06 had a good propertity in feed industry.
出处
《生物技术》
CAS
CSCD
2007年第1期20-23,共4页
Biotechnology
基金
浙江省重点科研农业项目资助(2006C22034)
关键词
米曲霉
中性蛋白酶
定向筛选
酶系分析
Aspergillus oryzae oryzae
neutral protease
screening
enzyme analysis
