摘要
中性蛋白酶作为水产饲料用酶受到广泛的关注。为获得高产中性蛋白酶,研究了米曲霉ZW-06产中性蛋白酶的培养基组分和培养条件,在此研究基础上,采用Ln18(37)正交试验设计,对影响米曲霉ZW-06菌株产中性蛋白酶的7个重要因素进一步优化。结果表明,米曲霉Aspergillus oryzaZW-06产中性蛋白酶的最适培养基组成为:在麸皮∶豆粕粉为8∶2的基础培养基中,添加0.5%Na2CO3;产酶最适培养条件为:培养温度30℃,培养基含水率60%(W/W),装量26.7 g/L,接种量20%(V/W),静置培养48 h,将获得最高中性蛋白酶产量,酶活达23 738 U/g。研究结果为中性蛋白酶的工业化生产提供了技术参数。
Abstract： Orthogonal design was applied to optimize fermentation conditions producing neutral protease from Aspergillus oryzae ZW-06. The seven factors including medium composition and cultivation conditions were chosen in the orthogonal design. The optimum composition in the cultmre medium was as follows ： 8 g of wheat bran and 2 g of soybean with adition of 0.5% Na2CO3 .The optimum temperature and moisture for producing neutral protease from the medium was 30.0℃ and 60 % （W/W）, respectively. About 26.7 g/L of the culture medium and 20 % （V/W） of inoculation volume were suitable for production of enzyme. 48 h of cultivation was enough for producing maximum neutral protease. Under optimum culture conditions, the activity of neutral protease from A. oryzae ZW-06 was 23 738 U/g.
出处
《浙江农业学报》
CSCD
2008年第6期451-456,共6页
Acta Agriculturae Zhejiangensis
基金
浙江省重点科研农业项目(2006C22034)
关键词
米曲霉
固态发酵
中性蛋白酶
正交试验
Aspergillus oryzae
solid fermentation
neutral protease
orthogonal design
