摘要
目的:探讨益母草生物碱和黄酮成分抗大鼠心肌缺血的药效学作用。方法:将实验大鼠随机分为正常组、模型组、益母草生物碱组、黄酮组、丹参组,及生物碱加黄酮大、中、小剂量组。采用大剂量异丙肾上腺素(ISO)皮下注射,造成大鼠急性心肌缺血损伤模型,用药后检测各组大鼠Ⅱ导联心电图J点变化情况、心肌组织形态学变化、血流动力学变化及酶谱变化。结果:与生物碱组、黄酮组及小剂量组相比,益母草生物碱加黄酮大、大中剂量组J点未发生明显偏移(P<0.05),左室内压峰值(LVSP)、左室内压最大变化速率(±dp/dtmax)向正常水平恢复(P<0.05),抑制损伤大鼠血清中肌酸磷酸激酶(CPK)、乳酸脱氢酶活性(LDH)活性及降低缺血心肌组织中丙二醛(MDA)的含量,提高大鼠心肌组织中超氧化物歧化酶(SOD)的活性(P<0.05),组织切片进一步说明生物碱和黄酮成分能有效保护心肌组织(P<0.05)。结论:益母草中的生物碱、黄酮成分能有效抑制异丙肾上腺素ISO所致心肌组织缺血损伤。
Objective. To observe the active ingredients of leonurus in myocardial isehemia induced by isoproterenol （ISO）. Methods： The model of myocardial ischemia was induced by subcutaneous injection of ISO （4 mg/kg for two days, once a day）. The experimental rats were randomly divided into normal group, ISO group, positive control group and leonurus composition groups. J Point in ECG, the histological changes of myocardium, the activity of LDH, CPK in serum and the level of MDA, SOD in myocardial tissue, and the LVSP, ±dp/dtmax were surveiled. Results： Compared with the model group, the leonurus composition groups had recovery effects on J point （ECG）, blood pressure, LVSP, and ±dp/dtmax, with decreased level of LDH, CPK, MDA, and increased activity of SOD. The histopathological changes confirmed the protection effects of alkaloid and ehromocor on the myocardial injury. Conclusion： The alkaloid and ehromocor are the effective ingredients of leonurus heterepyllus sweet, which can alleviate the acute myocardial isehemia caused by ISO in rats.
出处
《上海中医药大学学报》
CAS
2006年第1期61-63,共3页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
上海市科委科研基金资助项目(2003-B010)
关键词
益母草生物碱
黄酮
异丙肾上腺素
心肌缺血
Leonurus heteropyllus sweet
Alkaloid
chromocor
isepreterenol
myocardial ischemia
