摘要
用鸡胚脑神经细胞对１－烯丙基氯－３引起的细胞内游离Ｃａ^（２＋）、游离Ｎａ~＋的变化进行了研究。结果显示，在１－烯丙基氯－３的作用下，神经细胞内游离Ｃａ^（２＋）、游离Ｎａ~＋浓度随毒物浓度的增加而明显增高，呈显著的剂量－效应关系。细胞内游离Ｎａ~＋的改变在低剂量组无Ｃａ^（２＋）变化明显，细胞外高钾能使细胞内游离钙增加，但对细胞内游离Ｎａ~＋作用不明显。提示１－烯丙基氯－３引起的中毒性周围神经病可能与神经细胞内Ｎａ~＋、Ｃａ^（２＋）增加有关。
The present paper used fluorescence molecular probe Fura- 2 /AM and SBFI/AM, determined the changes of cytosolic ionized calcium ([ Ca2+ ] i) and cytosolic ionized sodium ([ Na+ ] i) in 14 day chicken embryon whole brain nerve cells. The results showed: [ Ca2+] i increased sharply with allyl chloride concentrations increased. The doseresponse relationship was obvious and had definite statistical significance (P<0.01, n= 4) . [Na+] i also added with allyl chloride concentrations increased. The statistical significance had marked difference (P<0.01, n=4) except the low dose of 40.72 mmol/L. The results can reveal that electrophysiological changes caused by allyl chloride may relate with Na+ and Ca2+ increased inside nerve cells.
出处
《山东医科大学学报》
1995年第3期210-213,共4页
Acta Academiae Medicinae Shandong
基金
国家自然科学基金
关键词
烯丙基氯
游离钠离子
神经毒理学
Allyl chloride
Cytosolic ionized calcium
Cytosolic ionized sodium
Neurotoxicology
