摘要
以牡丹"荷花紫"、"大胡红"品种的幼叶作外植体,采用正交设计法,考察6-BA、2,4-D、KT、NAA四种激素及酪蛋白对牡丹愈伤组织产生的影响,确定适宜的激素种类及水平,优化筛选出适宜于两个牡丹品种的愈伤组织诱导培养基.研究表明,激素2,4-D是幼叶愈伤诱导的关键因素;适宜于牡丹"荷花紫"及"大胡红"初培养的培养基配方均为:1/2MS+2,4-D4mg/L+KT0.2mg/L+NAA0.
Young leaves from subshrubby peony (paeonia suffruticosa) Hehuazi and Dahuhong are taken as explants.The effects of four kinds of hormones including 6-BA, 2, 4-D , KT ,NAA and Casein on peony callus initiation are studied.The proper callus induction medium is selected out by orthogonal design, moreover ,the kind and suitable level of hormone are ascertained.The results show that 2, 4-D is the critical factor to callus induction,the optimum medium for callus growth of both Hehuazi and Dahuhong is:1/2 MS+ 2, 4-D 4 mg/L+KT 0.2 mg/L+NAA 0.4 mg/L +CH 200 mg/L.
出处
《烟台大学学报（自然科学与工程版）》
CAS
2005年第1期41-44,49,共5页
Journal of Yantai University(Natural Science and Engineering Edition)
基金
山东省科技厅科技攻关项目资助(012010121).
关键词
牡丹
正交设计
愈伤组织
peony
orthogonal design
callus induction
