摘要
目的:从急性胰腺炎(AP)大鼠肠动力改变探中医中药急性胰腺炎治疗的以通为用思想的机制. 方法:由胆总管逆行注射15 g/L牛磺胆酸钠0.5 mL诱导大鼠AP,以红霉素为对照,并设假手术组空白对照,给模型大鼠灌喂通下解毒的代表方清胰汤,分别观察各组小肠动力学、血清胃动素(Mot)、血清淀粉酶(AML)和胰腺组织病理改变指标.并用正交设计的方法,观察了清胰汤不同药物对离体豚鼠结肠平滑肌条张力的影响. 结果:小肠推进比与AP组(0.21±0.08)相比较,清胰汤组(0.49±0.17)和红霉素组(0.30±0.10)明显提高(P<0.01), AP组血清MOT(119±24 pg)明显低于对照组(169±52 pg, P<0.05),清胰汤组(206±60 Pg)显著高于AP组(P<0.01). 清胰汤组的胰腺组织病理改变和血清AML显著改善.清胰汤各药对平滑肌有兴奋作用,对肌条张力影响的大小顺序是白芍、木香、延胡索和大黄. 结论:小肠动力障碍是AP发展的重要病理过程,中医以通为用、通下解毒的清胰汤能改善AP大鼠的胃肠动力的同时,减轻了AP的病理损伤.
AIM: To investigate the mechanism of the fluency-based therapy on gastrointestinal motility in rats with acute pancreatitis (AP). METHODS: AP rats were induced by choledochal retrograde injection of 15 g/L sodium taurocholate 0.5 mL. The treatment group was administered with Qingyitang orally as a representative prescription of the fluency-based therapy and erythromycin given orally as a control group. The gastrointestinal motility, motilin, amylase and related parameters of AP were observed. We introduced the trace multi-channel calorstat from Japan to observe the contraction effect of Qingyitang on colon longitudinal muscle strips with different component of Qingyitang according to the orthogonal designed methods (L12(211)) in vitro. RESULTS: The drive rate of small intestine on AP group was significantly reduced (0.21±0.08, P <0.01) with Qingyitang (0.49±0.17) and erythromycin groups (0.30±0.10); The serum concentration of motilin (119±24 pg) in AP group was significantly lower than that of normal control group (169±52 pg, P <0.05), While the concentration of Qingyitang group (206±60 pg) was significantly higher than that of AP group (P<0.01). Besides, the pancreatitis mass edema, inflammatory cell infiltration and serum amylase were significantly decreased. Radix glehnine, Radix aucklandiae, Rhizoma corydalis and Radix et Rhizoma rhei of Qingyitang could increase the tension of the muscle strip in vitro (P<0.05). CONCLUSION: The obstruction of gastrointestinal motility is an important pathologic process of AP, and Qingyitang as a representative prescription of the fluency-based therapy in traditional Chinese medicine, can improve the gastrointestinal motility in rats with AP.
出处
《世界华人消化杂志》
CAS
2004年第6期1369-1372,共4页
World Chinese Journal of Digestology
基金
日本岛津综合科学研究所国际合作基金
No.202JP1998~~
