Monitoring and delineating the spatial distribution of shale fracturing is fundamentally important to shale gas production. Standard monitoring methods, such as time-lapse seismic, cross-well seismic and micro-seismic...Monitoring and delineating the spatial distribution of shale fracturing is fundamentally important to shale gas production. Standard monitoring methods, such as time-lapse seismic, cross-well seismic and micro-seismic methods, are expensive, time- consuming, and do not show the changes in the formation with time. The resistivities of hydraulic fracturing fluid and reservoir rocks were measured. The results suggest that the injection fluid and consequently the injected reservoir are characterized by very low resistivity and high chargeability. This allows using of the controlled-source electromagnetic method （CSEM） to monitor shale gas hydraulic fracturing. Based on the geoelectrical model which was proposed according to the well-log and seismic data in the test area the change rule of the reacted electrical field was studied to account for the change of shale resistivity, and then the normalized residual resistivity method for time lapse processing was given. The time-domain electromagnetic method （TDEM） was used to continuously monitor the shale gas fracturing at the Fulin shale gas field in southern China. A high-power transmitter and multi-channel transient electromagnetic receiver array were adopted. 9 h time series of Ex component of 224 sites which were laid out on the surface and over three fracturing stages of a horizontal well at 2800 m depth was recorded. After data processing and calculation of the normalized resistivity residuals, the changes in the Ex signal were determined and a dynamic 3D image of the change in resistivity was constructed. This allows modeling the spatial distribution of the fracturing fluid. The model results suggest that TDEM is promising for monitoring hydraulic fracturing of shale.展开更多
Introduction Natural organic matter（NOM）present in source water has significant impact on water treatment processes and on the quality of drinking water.NOM is a complex mixture of diverse groups of organic compound...Introduction Natural organic matter（NOM）present in source water has significant impact on water treatment processes and on the quality of drinking water.NOM is a complex mixture of diverse groups of organic compounds,humic and fulvic acids,proteins,peptides,carbohydrates,and heterogeneous materials展开更多
Background There is a paucity of data about the best lipid ratio predicting the severity of coronary artery disease （CAD） in patients with diabetes mellitus. We determined the relationship between five conventional ...Background There is a paucity of data about the best lipid ratio predicting the severity of coronary artery disease （CAD） in patients with diabetes mellitus. We determined the relationship between five conventional lipid ratios and the extent of coronary artery lesions in Chinese Type 2 diabetics with stable angina pectoris （SAP）. Methods A prospective cohort study within 373 type 2 diabetic patients diag- nosed with stable CAD by coronary angiography was performed. All patients were classified into three groups according to the tertiles of Gensini scores （GS, low group 〈 8 points n = 143; intermediate group 8-28 points, n = 109; high group 〉 28 points, n = 121）. Association between the ratios of apolipoprotein （apo） B and apoA-1, total cholesterol and high density lipoprotein cholesterol （TC/HDL-C）, triglycerides and HDL-C （TG/HDL-C）, low density lipoprotein cholesterol and HDL-C （LDL-C/HDL-C）, Non-HDL-C/HDL-C and GS were evaluated using the receivers operating characteristic （ROC） curves and multivariate logistic regression models. Results The ratio of apoB/apoA-1, TC/HDL-C, LDL-C/HDL-C, and Non-HDL-C/HDL-C were correlated with Gensini scores. Area under the ROC curves for predicting high Gensini scores in the ratios of apoB/apoA-1, TC/HDL-C, LDL-C/HDL-C and Non-HDL-C/HDL-C were 0.62, 0.60, 0.59 and 0.60, respec- tively （P 〈 0.005 for all）. According to multivariate logistic regression analysis after adjusted with demographic characteristic and other lipid parameters, the ratio of apoB/apoA-1 is qualified as an independent discriminator for the severity of CAD. However, after fiu-ther adjusting different baseline variables, such as left ventricular ejective fraction, hemoglobin Alc, leukocytes count and serum creatinine, none of the above lipid ratios remained. Conclusions Compared with other lipid parameters, the ratio of apoB/apoA-1 appears to be more significantly correlated with the extent of coronary artery lesions in Chinese diabetics, but it was not an independen展开更多
Benzene is an established leukotoxin and leukemogen in humans. We have previously re- ported that exposure of workers to benzene and to benzene metabolite hydroquinone in cultured cells induced DNA-dependent protein k...Benzene is an established leukotoxin and leukemogen in humans. We have previously re- ported that exposure of workers to benzene and to benzene metabolite hydroquinone in cultured cells induced DNA-dependent protein kinase catalytic subunit （DNA-PKcs） to mediate the cellular response to DNA double strand break （DSB） caused by DNA-damaging metabolites. In this study, we used a new, small molecule, a selective inhibitor of DNA-PKcs, 2-（morpholin-4-yl）-benzo[h]chomen-4-one （NU7026）, as a probe to analyze the molecular events and pathways in hydroquinone-induced DNA DSB repair and apoptosis. Inhibition of DNA-PKcs by NU7026 markedly potentiated the apoptotic and growth inhibitory effects of hydroquinone in proerythroid leukemic K562 cells in a dose-dependent manner. Treatment with NU7026 did not alter the production of reactive oxygen species and oxidative stress by hydroquinone but repressed the protein level of DNA-PKcs and blocked the induction of the kinase mRNA and protein expression by hydroquinone. Moreover, hydroquinone increased the phos- phorylation of Akt to activate Akt, whereas co-treatment with NU7026 prevented the activation of Akt by hydroquinone. Lastly, hydroquinone and NU7026 exhibited synergistic effects on promoting apop- tosis by increasing the protein levels of pro-apoptotic proteins Bax and caspase-3 but decreasing the protein expression of anti-apoptotic protein Bcl-2. Taken together, the findings reveal a central role of DNA-PKcs in hydroquinone-induced hematotoxicity in which it coordinates DNA DSB repair, cell cycle progression, and apoptosis to regulate the response to hydroquinone-induced DNA damage.展开更多
The nanotechnology industry advances rapidly,and at the vanguard are the promising silver nanoparticles（Ag NPs）,which have diverse applications.These nanometer-sized particles have been shown to inhibit the ability ...The nanotechnology industry advances rapidly,and at the vanguard are the promising silver nanoparticles（Ag NPs）,which have diverse applications.These nanometer-sized particles have been shown to inhibit the ability of bacteria to produce adenosine triphosphate（ATP）,a molecule necessary for chemical energy transport in cells.The antimicrobial properties of Ag NPs（and Ag＋）make them valued antibacterial展开更多
Lithium metal(LM)is a promising anode for next-generation batteries due to its high theoretical capacity and low electrode potential.Nonetheless,side reactions,volume change,and unwanted lithium dendrite growth seriou...Lithium metal(LM)is a promising anode for next-generation batteries due to its high theoretical capacity and low electrode potential.Nonetheless,side reactions,volume change,and unwanted lithium dendrite growth seriously limit the practical application of LM.Herein,with the aid of a hard template approach,a novel lithiophilic CoF_(2)-carbon hollow sphere(CoF_(2)@C-HS)composite material is successfully prepared via a facile in-situ fluorination and etching strategy.The lithiophilic CoF_(2) acts as nucleation sites to reduce nucleation overpotential as well as induces the spatial Li deposition and the formation of LiFrich solid electrolyte interphase(SEI),and the hollow carbon matrix can enhance the electrical conductivity and offer free space for LM deposition.Theoretical simulations reveal that the synergistic effect of lithiophilic CoF_(2) and hollow carbon matrix homogenizes the electric field distribution and Li~+flux.Benefiting from these advantages,the CoF_(2)@C-HS-modified copper substrate electrode delivers an enhanced Coulombic efficiency(CE)of 93.7%for 280 cycles at 1 mA cm^(-2)and 1 mA h cm^(-2).The symmetrical cell using CoF_(2)@C-HS can stably cycle more than 1800 h with a low voltage hysteresis of 11 mV at a current density of 0.5 MA cm^(-2)and an areal capacity of 0.5 mA h cm^(-2).Moreover,the Li@CoF_(2)@C-HS composite anode enables more than 300 stable cycles at 1 C with a capacity retention of 95%in LiFePO_(4)-based full cell and 110 stable cycles at 1 C in LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2)(NCM811)-based highvoltage full cell.This work might shed a new light on designing lithiophilic hosts to spatially confine LM deposition,realizing dendrite-free LM anodes and the practical applications of LM batteries.展开更多
Background:In vivo experiments were conducted to examine the effects of the targeted drug anlotinib on the stemness of hepatocellular carcinoma(HCC)cells and lenvatinib-resistant liver cancer cells and to explore the ...Background:In vivo experiments were conducted to examine the effects of the targeted drug anlotinib on the stemness of hepatocellular carcinoma(HCC)cells and lenvatinib-resistant liver cancer cells and to explore the underlying molecular mechanisms.Methods:A subcutaneous xenograft model of Hep3B-derived HCC was established in nude mice,which were randomly divided into 2 groups(n=5 males per group):(1)intragastric administration of anlotinib(0.4 mg/kg)and(2)intragastric administration of normal saline.We constructed lenvatinib-resistant cell lines and randomly divided the mice into 3 groups(n=5 males per group):(1)intragastric administration of anlotinib,(2)intragastric administration of lenvatinib,and(3)intragastric administration of normal saline.After 2 weeks of treatment,tumor tissues were harvested,and mRNA and proteins were isolated from the tissues.Changes in the expression of cancer stemness markers(epithelial cell adhesion molecule[EpCAM],CD13,CD90,aldehyde dehydrogenase 1[ALDH1],CD44,and CD45),totipotency factors(sex-determining region Y-box 2[Sox2],Nanog,octamer-binding transcription factor 4[Oct4]),and genes related to the Notch signaling pathway were examined.Results:Compared with that in the control group,tumor size and weight were reduced in nude mice treated with anlotinib.These differences were statistically significant in both the types of nude mice.Anlotinib affected stemness markers and totipotency factors by downregulating the expression of CD133,CD90,and G-protein–coupled receptor 5(LGR5)and upregulating the expression of intercellular adhesion molecule 1(ICAM-1)and Sox2.In addition,lenvatinib-resistant cell lines increased Notch signaling pathway,whereas anlotinib inhibited Notch signaling pathway.Conclusions:The antitumor effect of anlotinib on HCC and lenvatinib-resistant HCC cellsmay occur through inhibition of the Notch signaling pathway.Anlotinib may be the drug of choice for sequential therapy in lenvatinib-resistant liver cancer.展开更多
Sodium metal,with a high theoretical specific capacity(~1165 mA h g^(−1))and a low redox potential(−2.71 V vs.SHE)as well as low cost,becomes an attractive option for high‐energy‐density sodium secondary batteries.H...Sodium metal,with a high theoretical specific capacity(~1165 mA h g^(−1))and a low redox potential(−2.71 V vs.SHE)as well as low cost,becomes an attractive option for high‐energy‐density sodium secondary batteries.However,the practical application of sodium metal anodes is hindered by dendrite growth,which results in low energy efficiency,poor lifetime and serious safety issues.To address this challenge,researchers propose various strategies,including the formation of sodium alloys(Na‐M alloys,M=Sn,Sb,Bi,In,etc.)through alloying reaction.The alloying effect has a positive impact in terms of reducing the local current density,mitigating the volume expansion,and inhibiting the dendrite growth.It is thus an effective solution for constructing high‐performance sodium secondary batteries.This review systematically de-scribes the mechanism of dendrite growth and the alloying process of Na‐M alloys,summarizes recent research progress and strategies for applying Na‐M alloys to create dendrite‐free sodium secondary batteries,as well as pre-sents prospects for the development of Na‐M alloys and offers clear sugges-tions for future research.This review aims to inspire further efforts to build dendrite‐free,high‐performance sodium secondary batteries and broaden a new aspect for the next‐generation battery systems.展开更多
Autophagy plays an important role in the interaction between viruses and host cells.SARS-CoV-2 infection can disrupt the autophagy process in target cells.However,the precise molecular mechanism is still unknown.In th...Autophagy plays an important role in the interaction between viruses and host cells.SARS-CoV-2 infection can disrupt the autophagy process in target cells.However,the precise molecular mechanism is still unknown.In this study,we discovered that the Nsp8 of SARS-CoV-2 could cause an increasing accumulation of autophagosomes by preventing the fusion of autophagosomes and lysosomes.From further investigation,we found that Nsp8 was present on mitochondria and can damage mitochondria to initiate mitophagy.The results of experiments with immunofluorescence revealed that Nsp8 induced incomplete mitophagy.Moreover,both domains of Nsp8 orchestrated their function during Nsp8-induced mitophagy,in which the N-terminal domain colocalized with mitochondria and the C-terminal domain induced auto/mitophagy.This novel finding expands our understanding of the function of Nsp8 in promoting mitochondrial damage and inducing incomplete mitophagy,which helps us to understand the etiology of COVID-19 as well as open up new pathways for creating SARS-CoV-2 treatment methods.展开更多
Arsenic(As)and antimony(Sb)rank 1 st and 244 th,respectively,on the 2019 Substance Priority List of the Agency for Toxic Substances and Disease Registry(ATSDR,2019).ATSDR lists substances,in order of priority,that are...Arsenic(As)and antimony(Sb)rank 1 st and 244 th,respectively,on the 2019 Substance Priority List of the Agency for Toxic Substances and Disease Registry(ATSDR,2019).ATSDR lists substances,in order of priority,that are"most commonly found at facilities on the National Priorities List(NPL)and which are determined to pose the most significant potential threat to human health due to their known or suspected toxicity and potential for human exposure at these NPL sites".展开更多
Objective Xiaoaiping (XAP) is a traditional Chinese medicine that is a commonly used as an anticancerdrug in clinical practice owing to its high efficiency and low toxicity. Specifically, XAP can effectively inhibitth...Objective Xiaoaiping (XAP) is a traditional Chinese medicine that is a commonly used as an anticancerdrug in clinical practice owing to its high efficiency and low toxicity. Specifically, XAP can effectively inhibitthe growth of hepatocellular carcinoma (HCC). Alpha-fetoprotein (AFP) is a key HCC diagnostic marker andis closely related to certain malignant cytological behaviors of HCC. However, whether AFP expression andXAP treatment are related to the invasion and metastasis of HCC remains unclear. In the present study, weaimed to evaluate the effects and underlying mechanism of XAP on the invasion and metastasis of HCC..Methods Using a cell scratch assay, Transwell technology, and western blotting we detected the differentinvasion and metastatic abilities of Hep3B cells in XAP treatment and blank control groups. This allowedcomparison of the invasion and metastatic abilities of Hep3B cells with differing levels of AFP expression.AFP mRNA sequencing technology was used to analyze the mechanism of tumor invasion and metastasisassociated with AFP and XAP treatment.Results Cell invasion and metastasis abilities in the XAP group were significantly lower than those in thecontrol group (P < 0.05). Additionally, compared to the control group, the expression of AFP significantlydecreased after XAP treatment (P < 0.05). The ability of Hep3B cells to invade and metastasize waspromoted when AFP expression was up-regulated, whereas it was inhibited when AFP was silenced. XAPinjection and AFP regulate the invasion and metastatic ability of HCC by affecting matrix metalloproteinases(MMPs).Conclusion XAP injection inhibits the invasion and metastatic ability of HCC by influencing the expressionof AFP;additionally, this inhibition of AFP is achieved by affecting MMPs.展开更多
Swimming is excellent exercise and offers many health benefits. However, the ＂chlorine smell＂ in swimming pools may be a turn-off for some people. Although this smell is often thought to be of chlorine, it actually ...Swimming is excellent exercise and offers many health benefits. However, the ＂chlorine smell＂ in swimming pools may be a turn-off for some people. Although this smell is often thought to be of chlorine, it actually comes from volatile compounds that are produced from unintended reactions between disinfectants （e.g., chlorine） and organic matter in the water （Li and Blatchley, 2007; Zwiener et al., 2007; Schmalz et al., 2011; Daiber et al., 2016）. Body fluids, such as sweat and urine, are among the sources of this organic matter that contribute to the formation of disinfection byproducts （DBPs） （Richardson et al., 2007; Arnaud, 2016; Tang et al., 2016）.展开更多
Halobenzoquinones(HBQs) are emerging disinfection byproducts(DBPs) with a widespread presence in drinking water that exhibit much higher cytotoxicity than regulated DBPs. However, the developmental neurotoxicity of HB...Halobenzoquinones(HBQs) are emerging disinfection byproducts(DBPs) with a widespread presence in drinking water that exhibit much higher cytotoxicity than regulated DBPs. However, the developmental neurotoxicity of HBQs has not been studied in vivo. In this work, we studied the neurotoxicity of HBQs on zebrafish embryos, after exposure to varying concentrations(0-8 μmol/L) of three HBQs, 2,5-dichloro-1,4-benzoquinone(2,5-DCBQ), 2,6-dichloro-1,4-benzoquinone(2,6-DCBQ), and 2,5-dibromo-1,4-benzoquinone(2,5-DBBQ) for 4 to 120 hr post fertilization(hpf). HBQ exposure significantly decreased the locomotor activity of larvae, accompanied by significant reduction of neurotransmitters(dopamine and γ-aminobutyric acid) and acetylcholinesterase activity. Furthermore, the expression of genes involved in neuronal morphogenesis( gfap, α1-tubulin, mbp, and syn-2 α) were downregulated by 4.4-, 5.2-, 3.0-, and 4.5-fold in the 5 μmol/L 2,5-DCBQ group and 2.0-, 1.6-, 2.1-, and 2.3-fold in the 5 μmol/L 2,5-DBBQ group, respectively. Transcriptomic analysis revealed that HBQ exposure affected the signaling pathways of neural development. This study demonstrates the significant neurotoxicity of HBQs in embryonic zebrafish and provides molecular evidence for understanding the potential mechanisms of HBQ neurotoxicity.展开更多
The Influence of ultrasonic treatment on the coking amount of a nickel-based catalyst （Ni/γ-Al2O3） for the reaction of reforming with carbon dioxide of Benzene was investigated. The results show that ultrasonic tre...The Influence of ultrasonic treatment on the coking amount of a nickel-based catalyst （Ni/γ-Al2O3） for the reaction of reforming with carbon dioxide of Benzene was investigated. The results show that ultrasonic treatment modify the pore size distribution of the catalysts significantly and also reduce the amount of coke formed on the catalyst. The reduction in the coking amount is not sensitive to the power output of the ultrasonic treatment device in the power range tested （120 W and 500 W）.展开更多
基金supported by NSFC(Grant No.U1562109 and 41774082)the National Major Research Plan(Grant No.2016YFC0601100and 2016ZX05004)the Project of Scientific Research and Technological Development,CNPC(Grant No.2017D-5006-16)
文摘Monitoring and delineating the spatial distribution of shale fracturing is fundamentally important to shale gas production. Standard monitoring methods, such as time-lapse seismic, cross-well seismic and micro-seismic methods, are expensive, time- consuming, and do not show the changes in the formation with time. The resistivities of hydraulic fracturing fluid and reservoir rocks were measured. The results suggest that the injection fluid and consequently the injected reservoir are characterized by very low resistivity and high chargeability. This allows using of the controlled-source electromagnetic method （CSEM） to monitor shale gas hydraulic fracturing. Based on the geoelectrical model which was proposed according to the well-log and seismic data in the test area the change rule of the reacted electrical field was studied to account for the change of shale resistivity, and then the normalized residual resistivity method for time lapse processing was given. The time-domain electromagnetic method （TDEM） was used to continuously monitor the shale gas fracturing at the Fulin shale gas field in southern China. A high-power transmitter and multi-channel transient electromagnetic receiver array were adopted. 9 h time series of Ex component of 224 sites which were laid out on the surface and over three fracturing stages of a horizontal well at 2800 m depth was recorded. After data processing and calculation of the normalized resistivity residuals, the changes in the Ex signal were determined and a dynamic 3D image of the change in resistivity was constructed. This allows modeling the spatial distribution of the fracturing fluid. The model results suggest that TDEM is promising for monitoring hydraulic fracturing of shale.
基金supported by the Natural Sciences and Engineering Research Council of Canada(NSERC)the National Natural Science Foundation of China,Alberta Innovates,and Alberta Health
文摘Introduction Natural organic matter（NOM）present in source water has significant impact on water treatment processes and on the quality of drinking water.NOM is a complex mixture of diverse groups of organic compounds,humic and fulvic acids,proteins,peptides,carbohydrates,and heterogeneous materials
文摘Background There is a paucity of data about the best lipid ratio predicting the severity of coronary artery disease （CAD） in patients with diabetes mellitus. We determined the relationship between five conventional lipid ratios and the extent of coronary artery lesions in Chinese Type 2 diabetics with stable angina pectoris （SAP）. Methods A prospective cohort study within 373 type 2 diabetic patients diag- nosed with stable CAD by coronary angiography was performed. All patients were classified into three groups according to the tertiles of Gensini scores （GS, low group 〈 8 points n = 143; intermediate group 8-28 points, n = 109; high group 〉 28 points, n = 121）. Association between the ratios of apolipoprotein （apo） B and apoA-1, total cholesterol and high density lipoprotein cholesterol （TC/HDL-C）, triglycerides and HDL-C （TG/HDL-C）, low density lipoprotein cholesterol and HDL-C （LDL-C/HDL-C）, Non-HDL-C/HDL-C and GS were evaluated using the receivers operating characteristic （ROC） curves and multivariate logistic regression models. Results The ratio of apoB/apoA-1, TC/HDL-C, LDL-C/HDL-C, and Non-HDL-C/HDL-C were correlated with Gensini scores. Area under the ROC curves for predicting high Gensini scores in the ratios of apoB/apoA-1, TC/HDL-C, LDL-C/HDL-C and Non-HDL-C/HDL-C were 0.62, 0.60, 0.59 and 0.60, respec- tively （P 〈 0.005 for all）. According to multivariate logistic regression analysis after adjusted with demographic characteristic and other lipid parameters, the ratio of apoB/apoA-1 is qualified as an independent discriminator for the severity of CAD. However, after fiu-ther adjusting different baseline variables, such as left ventricular ejective fraction, hemoglobin Alc, leukocytes count and serum creatinine, none of the above lipid ratios remained. Conclusions Compared with other lipid parameters, the ratio of apoB/apoA-1 appears to be more significantly correlated with the extent of coronary artery lesions in Chinese diabetics, but it was not an independen
文摘Benzene is an established leukotoxin and leukemogen in humans. We have previously re- ported that exposure of workers to benzene and to benzene metabolite hydroquinone in cultured cells induced DNA-dependent protein kinase catalytic subunit （DNA-PKcs） to mediate the cellular response to DNA double strand break （DSB） caused by DNA-damaging metabolites. In this study, we used a new, small molecule, a selective inhibitor of DNA-PKcs, 2-（morpholin-4-yl）-benzo[h]chomen-4-one （NU7026）, as a probe to analyze the molecular events and pathways in hydroquinone-induced DNA DSB repair and apoptosis. Inhibition of DNA-PKcs by NU7026 markedly potentiated the apoptotic and growth inhibitory effects of hydroquinone in proerythroid leukemic K562 cells in a dose-dependent manner. Treatment with NU7026 did not alter the production of reactive oxygen species and oxidative stress by hydroquinone but repressed the protein level of DNA-PKcs and blocked the induction of the kinase mRNA and protein expression by hydroquinone. Moreover, hydroquinone increased the phos- phorylation of Akt to activate Akt, whereas co-treatment with NU7026 prevented the activation of Akt by hydroquinone. Lastly, hydroquinone and NU7026 exhibited synergistic effects on promoting apop- tosis by increasing the protein levels of pro-apoptotic proteins Bax and caspase-3 but decreasing the protein expression of anti-apoptotic protein Bcl-2. Taken together, the findings reveal a central role of DNA-PKcs in hydroquinone-induced hematotoxicity in which it coordinates DNA DSB repair, cell cycle progression, and apoptosis to regulate the response to hydroquinone-induced DNA damage.
文摘The nanotechnology industry advances rapidly,and at the vanguard are the promising silver nanoparticles（Ag NPs）,which have diverse applications.These nanometer-sized particles have been shown to inhibit the ability of bacteria to produce adenosine triphosphate（ATP）,a molecule necessary for chemical energy transport in cells.The antimicrobial properties of Ag NPs（and Ag＋）make them valued antibacterial
基金supported by the Natural Science Foundation of China (52277218)the Hubei Provincial Natural Science Foundation of China (2024AFA094)+1 种基金the Excellent Discipline Cultivation Project by JHUN (2023XKZ009)supported by the U.S.Department of Energy,Office of Science,Office of Basic Energy Sciences,Materials Sciences and Engineering Division under contract number DE-AC05-00OR22725。
文摘Lithium metal(LM)is a promising anode for next-generation batteries due to its high theoretical capacity and low electrode potential.Nonetheless,side reactions,volume change,and unwanted lithium dendrite growth seriously limit the practical application of LM.Herein,with the aid of a hard template approach,a novel lithiophilic CoF_(2)-carbon hollow sphere(CoF_(2)@C-HS)composite material is successfully prepared via a facile in-situ fluorination and etching strategy.The lithiophilic CoF_(2) acts as nucleation sites to reduce nucleation overpotential as well as induces the spatial Li deposition and the formation of LiFrich solid electrolyte interphase(SEI),and the hollow carbon matrix can enhance the electrical conductivity and offer free space for LM deposition.Theoretical simulations reveal that the synergistic effect of lithiophilic CoF_(2) and hollow carbon matrix homogenizes the electric field distribution and Li~+flux.Benefiting from these advantages,the CoF_(2)@C-HS-modified copper substrate electrode delivers an enhanced Coulombic efficiency(CE)of 93.7%for 280 cycles at 1 mA cm^(-2)and 1 mA h cm^(-2).The symmetrical cell using CoF_(2)@C-HS can stably cycle more than 1800 h with a low voltage hysteresis of 11 mV at a current density of 0.5 MA cm^(-2)and an areal capacity of 0.5 mA h cm^(-2).Moreover,the Li@CoF_(2)@C-HS composite anode enables more than 300 stable cycles at 1 C with a capacity retention of 95%in LiFePO_(4)-based full cell and 110 stable cycles at 1 C in LiNi_(0.8)Co_(0.1)Mn_(0.1)O_(2)(NCM811)-based highvoltage full cell.This work might shed a new light on designing lithiophilic hosts to spatially confine LM deposition,realizing dendrite-free LM anodes and the practical applications of LM batteries.
基金supported by the Natural Science Foundation of the Hubei Province(no.2023 AFB894)Open for the Key Laboratory of Biological Targeted Therapy in 2021(no.2021swbx019).
文摘Background:In vivo experiments were conducted to examine the effects of the targeted drug anlotinib on the stemness of hepatocellular carcinoma(HCC)cells and lenvatinib-resistant liver cancer cells and to explore the underlying molecular mechanisms.Methods:A subcutaneous xenograft model of Hep3B-derived HCC was established in nude mice,which were randomly divided into 2 groups(n=5 males per group):(1)intragastric administration of anlotinib(0.4 mg/kg)and(2)intragastric administration of normal saline.We constructed lenvatinib-resistant cell lines and randomly divided the mice into 3 groups(n=5 males per group):(1)intragastric administration of anlotinib,(2)intragastric administration of lenvatinib,and(3)intragastric administration of normal saline.After 2 weeks of treatment,tumor tissues were harvested,and mRNA and proteins were isolated from the tissues.Changes in the expression of cancer stemness markers(epithelial cell adhesion molecule[EpCAM],CD13,CD90,aldehyde dehydrogenase 1[ALDH1],CD44,and CD45),totipotency factors(sex-determining region Y-box 2[Sox2],Nanog,octamer-binding transcription factor 4[Oct4]),and genes related to the Notch signaling pathway were examined.Results:Compared with that in the control group,tumor size and weight were reduced in nude mice treated with anlotinib.These differences were statistically significant in both the types of nude mice.Anlotinib affected stemness markers and totipotency factors by downregulating the expression of CD133,CD90,and G-protein–coupled receptor 5(LGR5)and upregulating the expression of intercellular adhesion molecule 1(ICAM-1)and Sox2.In addition,lenvatinib-resistant cell lines increased Notch signaling pathway,whereas anlotinib inhibited Notch signaling pathway.Conclusions:The antitumor effect of anlotinib on HCC and lenvatinib-resistant HCC cellsmay occur through inhibition of the Notch signaling pathway.Anlotinib may be the drug of choice for sequential therapy in lenvatinib-resistant liver cancer.
基金National Natural Science Foundation of China,Grant/Award Numbers:51972067,52222210。
文摘Sodium metal,with a high theoretical specific capacity(~1165 mA h g^(−1))and a low redox potential(−2.71 V vs.SHE)as well as low cost,becomes an attractive option for high‐energy‐density sodium secondary batteries.However,the practical application of sodium metal anodes is hindered by dendrite growth,which results in low energy efficiency,poor lifetime and serious safety issues.To address this challenge,researchers propose various strategies,including the formation of sodium alloys(Na‐M alloys,M=Sn,Sb,Bi,In,etc.)through alloying reaction.The alloying effect has a positive impact in terms of reducing the local current density,mitigating the volume expansion,and inhibiting the dendrite growth.It is thus an effective solution for constructing high‐performance sodium secondary batteries.This review systematically de-scribes the mechanism of dendrite growth and the alloying process of Na‐M alloys,summarizes recent research progress and strategies for applying Na‐M alloys to create dendrite‐free sodium secondary batteries,as well as pre-sents prospects for the development of Na‐M alloys and offers clear sugges-tions for future research.This review aims to inspire further efforts to build dendrite‐free,high‐performance sodium secondary batteries and broaden a new aspect for the next‐generation battery systems.
基金supported by the National Natural Science Foundation of China (grant numbers 32100131 and 31670716)Wuhan Science and Technology Bureau[grant numbers 2020020601012318]+1 种基金Hubei Key Laboratory of Wudang Local Chinese Medicine Research (Hubei University of Medicine) (grant numbers WDCM2022008)Jianghan University (grant numbers 08190006,06210035,2021yb138 and 2019037).
文摘Autophagy plays an important role in the interaction between viruses and host cells.SARS-CoV-2 infection can disrupt the autophagy process in target cells.However,the precise molecular mechanism is still unknown.In this study,we discovered that the Nsp8 of SARS-CoV-2 could cause an increasing accumulation of autophagosomes by preventing the fusion of autophagosomes and lysosomes.From further investigation,we found that Nsp8 was present on mitochondria and can damage mitochondria to initiate mitophagy.The results of experiments with immunofluorescence revealed that Nsp8 induced incomplete mitophagy.Moreover,both domains of Nsp8 orchestrated their function during Nsp8-induced mitophagy,in which the N-terminal domain colocalized with mitochondria and the C-terminal domain induced auto/mitophagy.This novel finding expands our understanding of the function of Nsp8 in promoting mitochondrial damage and inducing incomplete mitophagy,which helps us to understand the etiology of COVID-19 as well as open up new pathways for creating SARS-CoV-2 treatment methods.
文摘Arsenic(As)and antimony(Sb)rank 1 st and 244 th,respectively,on the 2019 Substance Priority List of the Agency for Toxic Substances and Disease Registry(ATSDR,2019).ATSDR lists substances,in order of priority,that are"most commonly found at facilities on the National Priorities List(NPL)and which are determined to pose the most significant potential threat to human health due to their known or suspected toxicity and potential for human exposure at these NPL sites".
文摘Objective Xiaoaiping (XAP) is a traditional Chinese medicine that is a commonly used as an anticancerdrug in clinical practice owing to its high efficiency and low toxicity. Specifically, XAP can effectively inhibitthe growth of hepatocellular carcinoma (HCC). Alpha-fetoprotein (AFP) is a key HCC diagnostic marker andis closely related to certain malignant cytological behaviors of HCC. However, whether AFP expression andXAP treatment are related to the invasion and metastasis of HCC remains unclear. In the present study, weaimed to evaluate the effects and underlying mechanism of XAP on the invasion and metastasis of HCC..Methods Using a cell scratch assay, Transwell technology, and western blotting we detected the differentinvasion and metastatic abilities of Hep3B cells in XAP treatment and blank control groups. This allowedcomparison of the invasion and metastatic abilities of Hep3B cells with differing levels of AFP expression.AFP mRNA sequencing technology was used to analyze the mechanism of tumor invasion and metastasisassociated with AFP and XAP treatment.Results Cell invasion and metastasis abilities in the XAP group were significantly lower than those in thecontrol group (P < 0.05). Additionally, compared to the control group, the expression of AFP significantlydecreased after XAP treatment (P < 0.05). The ability of Hep3B cells to invade and metastasize waspromoted when AFP expression was up-regulated, whereas it was inhibited when AFP was silenced. XAPinjection and AFP regulate the invasion and metastatic ability of HCC by affecting matrix metalloproteinases(MMPs).Conclusion XAP injection inhibits the invasion and metastatic ability of HCC by influencing the expressionof AFP;additionally, this inhibition of AFP is achieved by affecting MMPs.
文摘Swimming is excellent exercise and offers many health benefits. However, the ＂chlorine smell＂ in swimming pools may be a turn-off for some people. Although this smell is often thought to be of chlorine, it actually comes from volatile compounds that are produced from unintended reactions between disinfectants （e.g., chlorine） and organic matter in the water （Li and Blatchley, 2007; Zwiener et al., 2007; Schmalz et al., 2011; Daiber et al., 2016）. Body fluids, such as sweat and urine, are among the sources of this organic matter that contribute to the formation of disinfection byproducts （DBPs） （Richardson et al., 2007; Arnaud, 2016; Tang et al., 2016）.
基金the National Natural Science Foundation of China (Nos. 21677062, 21607059 and 21507155)the Scientific and Technological Innovation Special Project of Jianghan University (No. 2021KJZX002)+1 种基金the Natural Sciences and Engineering Research Council of Canada, the Canada Research Chairs Program, Alberta Innovates, and Alberta Health for their supportthe support of the China Scholarship Council。
文摘Halobenzoquinones(HBQs) are emerging disinfection byproducts(DBPs) with a widespread presence in drinking water that exhibit much higher cytotoxicity than regulated DBPs. However, the developmental neurotoxicity of HBQs has not been studied in vivo. In this work, we studied the neurotoxicity of HBQs on zebrafish embryos, after exposure to varying concentrations(0-8 μmol/L) of three HBQs, 2,5-dichloro-1,4-benzoquinone(2,5-DCBQ), 2,6-dichloro-1,4-benzoquinone(2,6-DCBQ), and 2,5-dibromo-1,4-benzoquinone(2,5-DBBQ) for 4 to 120 hr post fertilization(hpf). HBQ exposure significantly decreased the locomotor activity of larvae, accompanied by significant reduction of neurotransmitters(dopamine and γ-aminobutyric acid) and acetylcholinesterase activity. Furthermore, the expression of genes involved in neuronal morphogenesis( gfap, α1-tubulin, mbp, and syn-2 α) were downregulated by 4.4-, 5.2-, 3.0-, and 4.5-fold in the 5 μmol/L 2,5-DCBQ group and 2.0-, 1.6-, 2.1-, and 2.3-fold in the 5 μmol/L 2,5-DBBQ group, respectively. Transcriptomic analysis revealed that HBQ exposure affected the signaling pathways of neural development. This study demonstrates the significant neurotoxicity of HBQs in embryonic zebrafish and provides molecular evidence for understanding the potential mechanisms of HBQ neurotoxicity.
基金Supported by National ＂211＂ Key Discipline Development Program, Natural Science Foundation of Hubei Province（2006AB 192）, Science Foundation of Wuhan（20066002064）, China.
文摘The Influence of ultrasonic treatment on the coking amount of a nickel-based catalyst （Ni/γ-Al2O3） for the reaction of reforming with carbon dioxide of Benzene was investigated. The results show that ultrasonic treatment modify the pore size distribution of the catalysts significantly and also reduce the amount of coke formed on the catalyst. The reduction in the coking amount is not sensitive to the power output of the ultrasonic treatment device in the power range tested （120 W and 500 W）.
