Background:Recognizing sport-related concussion(SRC)is challenging and relies heavily on subjective symptom reports.An objective,biological marker could improve recognition and understanding of SRC.There is emerging e...Background:Recognizing sport-related concussion(SRC)is challenging and relies heavily on subjective symptom reports.An objective,biological marker could improve recognition and understanding of SRC.There is emerging evidence that salivary micro-ribonucleic acids(miRNAs)may serve as biomarkers of concussion;however,it remains unclear whether concussion-related miRNAs are impacted by exercise.We sought to determine whether40 miRNAs previously implicated in concussion pathophysiology were affected by participation in a variety of contact and non-contact sports.Our goal was to refine a miRNA-based tool capable of identifying athletes with SRC without the confounding effects of exercise.Methods:This case-control study harmonized data from concussed and non-concussed athletes recruited across 10 sites.Levels of salivary miRNAs within 455 samples from 314 individuals were measured with RNA sequencing.Within-subjects testing was used to identify and exclude miRNAs that changed with either(a)a single episode of exercise(166 samples from 83 individuals)or(b)season-long participation in contact sports(212 samples from 106 individuals).The miRNAs that were not impacted by exercise were interrogated for SRC diagnostic utility using logistic regression(172 samples from 75 concussed and 97 non-concussed individuals).Results:Two miRNAs(miR-532-5p and miR-182-5p)decreased(adjusted p<0.05)after a single episode of exercise,and 1 miRNA(miR-4510)increased only after contact sports participation.Twenty-three miRNAs changed at the end of a contact sports season.Two of these miRNAs(miR-26b-3p and miR-29c-3p)were associated(R>0.50;adjusted p<0.05)with the number of head impacts sustained in a single football practice.Among the 15 miRNAs not confounded by exercise or season-long contact sports participation,11 demonstrated a significant difference(adjusted p<0.05)between concussed and non-concussed participants,and 6 displayed moderate ability(area under curve>0.70)to identify concussion.A single ratio(miR-27a-5p/miR-30a-3p)displayed the展开更多
Osteoporosis is a metabolic dysregulation of bone that occurs mainly in postmenopausal women,and the hyperfunction of osteoclasts is the primary contributor to postmenopausal osteoporosis.However,the development of ef...Osteoporosis is a metabolic dysregulation of bone that occurs mainly in postmenopausal women,and the hyperfunction of osteoclasts is the primary contributor to postmenopausal osteoporosis.However,the development of effective therapeutic drugs and precise delivery systems remains a challenge in the field of anti-absorption therapy.Here,we reported theα-cyperone(α-CYP)for anti-osteoporosis and developed a liposome-based nano-drug delivery system ofα-CYP,that specifically targets the bone resorption interface.Firstly,we found that theα-CYP,one of the major sesquiterpenes of Cyperus rotundus L.,attenuated the progression of osteoporosis in ovariectomized(OVX)mice and down-regulated the expression of phosphorylated proteins of phosphoinositide 3-kinase(PI3K)and protein kinase B(Akt),causing down-regulation of osteoclast-related genes/proteins and curbing osteoclast differentiation.Furthermore,α-CYP reversed the activation of osteoclastic differentiation and enhanced osteoporosis-related proteins expression caused by PI3K/Akt agonist(YS-49).More importantly,we adopted the osteoclastic resorption surface targeting peptide Asp8 and constructed the liposome(lipαC@Asp8)to deliverα-CYP to osteoclasts and confirmed its anti-osteoporosis effect and enhanced osteoclast inhibition by blocking PI3K/Akt axis.In conclusion,this study demonstrated thatα-CYP inhibits osteoclast differentiation and osteoporosis development by silencing PI3K/Akt pathway,and the liposome targeting delivery systems loaded withα-CYP might provide a novel and effective strategy to treat osteoporosis.展开更多
Diabetic osteoporosis(DOP)is a serious complication of diabetes mellitus.It is urgent to explore efficient clinical treatment strategies for DOP.It has been found that mesenchymal stem cell-derived extracellular vesic...Diabetic osteoporosis(DOP)is a serious complication of diabetes mellitus.It is urgent to explore efficient clinical treatment strategies for DOP.It has been found that mesenchymal stem cell-derived extracellular vesicles(MSC-EVs),as an emerging cell-free therapy,show great potential in DOP treatment.MSC-EVs can effectively promote bone formation,inhibit bone resorption,and modulate the inflammatory microenvironment by delivering cargoes of microRNAs,long non-coding RNAs,and proteins to target cells,thereby ameliorating bone loss in DOP.However,there are limited reports on the treatment of DOP with MSC-EVs.To evoke more attention to this potential strategy,this article summarised the extant literature on MSC-EVs for DOP to provide new directions for further research and to promote the application of MSC-EVs in the clinical management of DOP.展开更多
Autophagy,as a fundamental mechanism for cellular homeostasis,is generally involved in the occurrence and progression of various diseases.Osteoarthritis(OA)is the most common muscu-loskeletal disease that often leads ...Autophagy,as a fundamental mechanism for cellular homeostasis,is generally involved in the occurrence and progression of various diseases.Osteoarthritis(OA)is the most common muscu-loskeletal disease that often leads to pain,disability and economic loss in patients.Post-traumatic OA(PTOA)is a subtype of OA,accounting for>12%of the overall burden of OA.PTOA is often caused by joint injuries including anterior cruciate ligament rupture,meniscus tear and intra-articular fracture.Although a variety of methods have been developed to treat acute joint injury,the current measures have limited success in effectively reducing the incidence and delaying the progression of PTOA.Therefore,the pathogenesis and intervention strategy of PTOA need further study.In the past decade,the roles and mechanisms of autophagy in PTOA have aroused great interest in the field.It was revealed that autophagy could maintain the homeostasis of chondrocytes,reduce joint inflammatory level,prevent chondrocyte death and matrix degradation,which accordingly improved joint symptoms and delayed the progression of PTOA.Moreover,many strategies that target PTOA have been revealed to promote autophagy.In this review,we summarize the roles and mechanisms of autophagy in PTOA and the current strategies for PTOA treatment that depend on autophagy regulation,which may be beneficial for PTOA patients in the future.展开更多
Enhancing the regeneration of cartilage defects remains challenging owing to limited innate self-healing as well as acute inflammation arising from the overexpression of reactive oxygen species(ROS)in post-traumatic m...Enhancing the regeneration of cartilage defects remains challenging owing to limited innate self-healing as well as acute inflammation arising from the overexpression of reactive oxygen species(ROS)in post-traumatic microenvironments.Recently,stem cell-derived exosomes(Exos)have been developed as potential cell-free therapy for cartilage regeneration.Although this approach promotes chondrogenesis,it neglects the emerging inflammatory microenvironment.In this study,a smart bilayer-hydrogel dual-loaded with sodium diclofenac(DC),an anti-inflammatory drug,and Exos from bone marrow-derived mesenchymal stem cells was developed to mitigate initial-stage inflammation and promote late-stage stem-cell recruitment and chondrogenic differentiation.First,the upper-hydrogel composed of phenylboronic-acid-crosslinked polyvinyl alcohol degrades in response to elevated levels of ROS to release DC,which mitigates oxidative stress,thus reprogramming macrophages to the pro-healing state.Subsequently,Exos are slowly released from the lower-hydrogel composed of hyaluronic acid into an optimal microenvironment for the stimulation of chondrogenesis.Both in vitro and in vivo assays confirmed that the dual-loaded bilayer-hydrogel reduced post-traumatic inflammation and enhanced cartilage regeneration by effectively scavenging ROS and reprogramming macrophages.The proposed platform provides multi-staged therapy,which allows for the optimal harnessing of Exos as a therapeutic for cartilage regeneration.展开更多
The role of tourism in social and economic development and stabilizing the Olympic legacy has been widely discussed.Beijing is the first“Dual-Olympic City”.For the first time,the Beijing Winter Olympics has adopted ...The role of tourism in social and economic development and stabilizing the Olympic legacy has been widely discussed.Beijing is the first“Dual-Olympic City”.For the first time,the Beijing Winter Olympics has adopted a model of three competition areas,which has attracted much attention to the study of its tourism legacy.In the legacy plan,the construction of the“Beijing-Zhangjiakou Sports Culture and Tourism Belt”was proposed.The development of ice and snow tourism and the cultivation of the ice and snow tourism market is also reflected in many policy documents and government actions.The scientific planning before the competition laid a good foundation for the sustainable development of its ice and snow tourism heritage.This research combines Olympic legacy research with tourism destination theory,and focuses on ice and snow tourism directly related to the Winter Olympics and the significance of pre-event planning for legacy protection.At the same time,the improvement of the quality of ice and snow tourism destinations is embedded in the pre-Olympic legacy,and opinions and suggestions on how to ensure the stability of the legacy after the games are given.This study uses the literature review method and the second-hand materials survey research method,then through establishing a tourism legacy pre-game evaluation model and the use of geographic information,government reports,policies,publicly released statistics and news reports,etc..It explores the legacy of the Beijing 2022 Winter Olympics and Paralympics,aiming to lay the foundation for preserving the continuous value of the ice and snow tourism legacy during and after the Olympics.The results indicate that the Beijing Winter Olympics has made relatively complete plans and reliable progress in both tangible and intangible legacy.Complete tourism infrastructure,an optimistic sport and cultural atmosphere,and the improvement of residents’health concept and sports awareness have become boosters for the development of ice and snow tourism in the Beijing-Zha展开更多
Beijing will host the 2022 Winter Olympics,and China strengthens research on various aspects to allow their athletes to compete successfully in winter sport.Simultaneously,Government-directed initiatives aim to increa...Beijing will host the 2022 Winter Olympics,and China strengthens research on various aspects to allow their athletes to compete successfully in winter sport.Simultaneously,Government-directed initiatives aim to increase public participation in recreational winter sport.These parallel developments allow research to advance knowledge and understanding of the physiological determinants of performance and health related to winter sport.Winter sport athletes often conduct a substan-tial amount of training with high volumes of low-to-moderate exercise intensity and lower volumes of high-intensity work.Moreover,much of the training occur at low ambient temperatures and winter sport athletes have high risk of developing asthma or asthma-related conditions,such as exercise-induced bronchoconstriction.The high training volumes require optimal nutrition with increased energy and dietary protein requirement to stimulate muscle protein synthesis response in the post-exercise period.Whether higher protein intake is required in the cold should be investigated.Cross-country ski-ing is performed mostly in Northern hemisphere with a strong cultural heritage and sporting tradition.It is expected that innovative initiatives on recruitment and training during the next few years will target to enhance performance of Chinese athletes in classical endurance-based winter sport.The innovation potential coupled with resourcing and population may be substantial with the potential for China to become a significant winter sport nation.This paper discusses the physiological aspects of endurance training and performance in winter sport highlighting areas where innovation may advance in athletic performance in cold environments.In addition,to ensure sustainable development of snow sport,a quality ski patrol and rescue system is recommended for the safety of increasing mass participation.展开更多
Wearable devices have been used in the treatment and rehabilitation of ankle injuries.This article systematically reviewed the trials that summarize and evaluate the effectiveness of rehabilitation treatment after an ...Wearable devices have been used in the treatment and rehabilitation of ankle injuries.This article systematically reviewed the trials that summarize and evaluate the effectiveness of rehabilitation treatment after an ankle injury.Three databases,PubMed(1974–2021),Embase,and Web of Science(1950–2021),were searched.The intervention was any wearable device,and the outcome measures were Activities Scale for Kids performance(ASKp),Foot and Ankle Outcome Score(FAOS),American Orthopaedic Foot and Ankle Society(AOFAS),Olerud-Molander Ankle Score(OMAS),and Circumference as measured by any validated outcome measure.Two independent authors evaluated the studies with the Cochrane risk-of-bias tool.Four papers were included,involving 476 participants,with a mean age of 29.3±6.7 years.The mean duration of wearable devices was 3.83 weeks,and the mean length of training was 3.75 weeks.Wearable devices achieved better results compared with control on the functional performance(standardized mean difference[SMD]0.66;95%confidence interval[CI]0.29 to 1.04;I^(2)=76%;P<0.001),as well as ankle score(SMD 0.78;95%CI 0.22 to 1.35;I^(2)=82%;P<0.001).The definitive judgment could not be made due to the variability in training,training duration,and outcomes measurement.Wearable devices are a promising approach that has positive effects on ankle injuries in terms of functional performance and reducing the extent of swelling.There is insufficient evidence from randomized controlled trials(RCTs)to support this for ankle injury patients using wearable devices.Therefore,there is an need for well-conducted randomized controlled trials investigating more adaptive orthoses to achieve more effective strategies for early functional rehabilitation.PROSPERO registration number:CRD42021246289.展开更多
The effects of Greyhound racing on plasma insulin, glucagon and non-esterified fatty acid have not been extensively studied. We sought to examine the effects of exercise in 19 greyhounds trialed over 400 meters on ser...The effects of Greyhound racing on plasma insulin, glucagon and non-esterified fatty acid have not been extensively studied. We sought to examine the effects of exercise in 19 greyhounds trialed over 400 meters on serum biochemistry, osmolality, nonessential fatty acids, and the major glucoregulatory hormones insulin and glucagon, before and after racing. Due to the non-traditional dietary patterns of many racing Greyhounds, the diet was analyzed for macro and micro-nutrient assessment to determine adequacy of the diet. Many significant biochemical changes were observed and agreed with previous studies, except for low resting glucose, low post-exercise globulin and phosphorus concentrations, and a high CK both before and after exercise. Insulin concentrations increased 10 minutes post-exercise which might be a result of the rise in serum glucose observed. Non-esterified fatty acids decreased 10 minutes post-exercise, as a likely result of the insulin facilitated cellular uptake of fatty acids early in recovery. Glucagon concentrations did not change after exercise despite the rise in insulin. The diet analysis found some mineral deficiencies in sodium, selenium, iodine and copper;and contained a far higher protein and fat content, being atypical of diets that have been shown to improve the performance in racing Greyhounds.展开更多
Introduction Organ transplantation increases survival and improves qual-ity of life to many patients with end-stage organ failure.Or-gan shortage is a worldwide problem that restricts organ trans-plantation[1].Organ p...Introduction Organ transplantation increases survival and improves qual-ity of life to many patients with end-stage organ failure.Or-gan shortage is a worldwide problem that restricts organ trans-plantation[1].Organ procurement and preservation as well as ischemia-reperfusion injury(IRI)after transplantation are the im-portant factors affecting prognosis of recipients.Since the de-velopment of organ transplantation technology in the 20th cen-tury,organ protection technology has been a most promising con-cept in this field.Organ preservation solutions such as the Collins solution,University of Wisconsin(UW)solution,and histidine-tryptophan-ketoglutarate(HTK)solution were developed sequen-tially[2],which developed rapidly in static cold storage(SCS)tech-niques.SCS remains the standard preservation technique for organ transplantation[2].展开更多
基金supported by a sponsored research agreement between Quadrant Biosciences and the Penn State College of Medicine to ACLsupported by the National Center for Advancing Translational Sciences(Grant KL2 TR002015,Grant UL1 TR002014)。
文摘Background:Recognizing sport-related concussion(SRC)is challenging and relies heavily on subjective symptom reports.An objective,biological marker could improve recognition and understanding of SRC.There is emerging evidence that salivary micro-ribonucleic acids(miRNAs)may serve as biomarkers of concussion;however,it remains unclear whether concussion-related miRNAs are impacted by exercise.We sought to determine whether40 miRNAs previously implicated in concussion pathophysiology were affected by participation in a variety of contact and non-contact sports.Our goal was to refine a miRNA-based tool capable of identifying athletes with SRC without the confounding effects of exercise.Methods:This case-control study harmonized data from concussed and non-concussed athletes recruited across 10 sites.Levels of salivary miRNAs within 455 samples from 314 individuals were measured with RNA sequencing.Within-subjects testing was used to identify and exclude miRNAs that changed with either(a)a single episode of exercise(166 samples from 83 individuals)or(b)season-long participation in contact sports(212 samples from 106 individuals).The miRNAs that were not impacted by exercise were interrogated for SRC diagnostic utility using logistic regression(172 samples from 75 concussed and 97 non-concussed individuals).Results:Two miRNAs(miR-532-5p and miR-182-5p)decreased(adjusted p<0.05)after a single episode of exercise,and 1 miRNA(miR-4510)increased only after contact sports participation.Twenty-three miRNAs changed at the end of a contact sports season.Two of these miRNAs(miR-26b-3p and miR-29c-3p)were associated(R>0.50;adjusted p<0.05)with the number of head impacts sustained in a single football practice.Among the 15 miRNAs not confounded by exercise or season-long contact sports participation,11 demonstrated a significant difference(adjusted p<0.05)between concussed and non-concussed participants,and 6 displayed moderate ability(area under curve>0.70)to identify concussion.A single ratio(miR-27a-5p/miR-30a-3p)displayed the
基金supported by the National Key Research and Development Project(No.2021YFA1201404)Major Project of the National Natural Science Foundation of China(Nos.81991514,82272530)+2 种基金Jiangsu Province Medical Innovation Center of Orthopedic Surgery(No.CXZX202214)Jiangsu Provincial Key Medical Center Foundation,Jiangsu Provincial Medical Outstanding Talent Foundation,Jiangsu Provincial Medical Youth Talent Foundation,Jiangsu Provincial Key Medical Talent Foundationthe Fundamental Research Funds for the Central Universities(Nos.14380493 and 14380494).
文摘Osteoporosis is a metabolic dysregulation of bone that occurs mainly in postmenopausal women,and the hyperfunction of osteoclasts is the primary contributor to postmenopausal osteoporosis.However,the development of effective therapeutic drugs and precise delivery systems remains a challenge in the field of anti-absorption therapy.Here,we reported theα-cyperone(α-CYP)for anti-osteoporosis and developed a liposome-based nano-drug delivery system ofα-CYP,that specifically targets the bone resorption interface.Firstly,we found that theα-CYP,one of the major sesquiterpenes of Cyperus rotundus L.,attenuated the progression of osteoporosis in ovariectomized(OVX)mice and down-regulated the expression of phosphorylated proteins of phosphoinositide 3-kinase(PI3K)and protein kinase B(Akt),causing down-regulation of osteoclast-related genes/proteins and curbing osteoclast differentiation.Furthermore,α-CYP reversed the activation of osteoclastic differentiation and enhanced osteoporosis-related proteins expression caused by PI3K/Akt agonist(YS-49).More importantly,we adopted the osteoclastic resorption surface targeting peptide Asp8 and constructed the liposome(lipαC@Asp8)to deliverα-CYP to osteoclasts and confirmed its anti-osteoporosis effect and enhanced osteoclast inhibition by blocking PI3K/Akt axis.In conclusion,this study demonstrated thatα-CYP inhibits osteoclast differentiation and osteoporosis development by silencing PI3K/Akt pathway,and the liposome targeting delivery systems loaded withα-CYP might provide a novel and effective strategy to treat osteoporosis.
文摘Diabetic osteoporosis(DOP)is a serious complication of diabetes mellitus.It is urgent to explore efficient clinical treatment strategies for DOP.It has been found that mesenchymal stem cell-derived extracellular vesicles(MSC-EVs),as an emerging cell-free therapy,show great potential in DOP treatment.MSC-EVs can effectively promote bone formation,inhibit bone resorption,and modulate the inflammatory microenvironment by delivering cargoes of microRNAs,long non-coding RNAs,and proteins to target cells,thereby ameliorating bone loss in DOP.However,there are limited reports on the treatment of DOP with MSC-EVs.To evoke more attention to this potential strategy,this article summarised the extant literature on MSC-EVs for DOP to provide new directions for further research and to promote the application of MSC-EVs in the clinical management of DOP.
基金funding by the National Natural Science Foundation of China(No.81871817,No.82202770,No.81802205)the Natural Science Foundation of Chongqing(No.CSTB2022NSCQMSX1267,No.CSTB2022NSCQ-MSX0863)+1 种基金the Laboratory Project of State Key Laboratory of Trauma,Burn and Combined Injury(No.SKL2021JY01)the Military Project of PLA(No.2020XYY11,No.21WQ002,No.2019HQZX03).
文摘Autophagy,as a fundamental mechanism for cellular homeostasis,is generally involved in the occurrence and progression of various diseases.Osteoarthritis(OA)is the most common muscu-loskeletal disease that often leads to pain,disability and economic loss in patients.Post-traumatic OA(PTOA)is a subtype of OA,accounting for>12%of the overall burden of OA.PTOA is often caused by joint injuries including anterior cruciate ligament rupture,meniscus tear and intra-articular fracture.Although a variety of methods have been developed to treat acute joint injury,the current measures have limited success in effectively reducing the incidence and delaying the progression of PTOA.Therefore,the pathogenesis and intervention strategy of PTOA need further study.In the past decade,the roles and mechanisms of autophagy in PTOA have aroused great interest in the field.It was revealed that autophagy could maintain the homeostasis of chondrocytes,reduce joint inflammatory level,prevent chondrocyte death and matrix degradation,which accordingly improved joint symptoms and delayed the progression of PTOA.Moreover,many strategies that target PTOA have been revealed to promote autophagy.In this review,we summarize the roles and mechanisms of autophagy in PTOA and the current strategies for PTOA treatment that depend on autophagy regulation,which may be beneficial for PTOA patients in the future.
基金supported by National Natural Science Foundation of China(Nos.52002223,81672185 and 81702152)Key Technology Research and Development Program of Shandong(Major Science and Technology Innovation Project)(2020CXGC010502)+1 种基金Taishan Scholar Foundation of Shandong Province(No.tsqn202211348)the Shandong Province Natural Science Foundation(Grant Nos.ZR2022MH222 and ZR2023MH209).
文摘Enhancing the regeneration of cartilage defects remains challenging owing to limited innate self-healing as well as acute inflammation arising from the overexpression of reactive oxygen species(ROS)in post-traumatic microenvironments.Recently,stem cell-derived exosomes(Exos)have been developed as potential cell-free therapy for cartilage regeneration.Although this approach promotes chondrogenesis,it neglects the emerging inflammatory microenvironment.In this study,a smart bilayer-hydrogel dual-loaded with sodium diclofenac(DC),an anti-inflammatory drug,and Exos from bone marrow-derived mesenchymal stem cells was developed to mitigate initial-stage inflammation and promote late-stage stem-cell recruitment and chondrogenic differentiation.First,the upper-hydrogel composed of phenylboronic-acid-crosslinked polyvinyl alcohol degrades in response to elevated levels of ROS to release DC,which mitigates oxidative stress,thus reprogramming macrophages to the pro-healing state.Subsequently,Exos are slowly released from the lower-hydrogel composed of hyaluronic acid into an optimal microenvironment for the stimulation of chondrogenesis.Both in vitro and in vivo assays confirmed that the dual-loaded bilayer-hydrogel reduced post-traumatic inflammation and enhanced cartilage regeneration by effectively scavenging ROS and reprogramming macrophages.The proposed platform provides multi-staged therapy,which allows for the optimal harnessing of Exos as a therapeutic for cartilage regeneration.
基金The Key Project of National Social Science Foundation (21ATY001)The National Natural Science Foundation of China Youth Project (42001255)
文摘The role of tourism in social and economic development and stabilizing the Olympic legacy has been widely discussed.Beijing is the first“Dual-Olympic City”.For the first time,the Beijing Winter Olympics has adopted a model of three competition areas,which has attracted much attention to the study of its tourism legacy.In the legacy plan,the construction of the“Beijing-Zhangjiakou Sports Culture and Tourism Belt”was proposed.The development of ice and snow tourism and the cultivation of the ice and snow tourism market is also reflected in many policy documents and government actions.The scientific planning before the competition laid a good foundation for the sustainable development of its ice and snow tourism heritage.This research combines Olympic legacy research with tourism destination theory,and focuses on ice and snow tourism directly related to the Winter Olympics and the significance of pre-event planning for legacy protection.At the same time,the improvement of the quality of ice and snow tourism destinations is embedded in the pre-Olympic legacy,and opinions and suggestions on how to ensure the stability of the legacy after the games are given.This study uses the literature review method and the second-hand materials survey research method,then through establishing a tourism legacy pre-game evaluation model and the use of geographic information,government reports,policies,publicly released statistics and news reports,etc..It explores the legacy of the Beijing 2022 Winter Olympics and Paralympics,aiming to lay the foundation for preserving the continuous value of the ice and snow tourism legacy during and after the Olympics.The results indicate that the Beijing Winter Olympics has made relatively complete plans and reliable progress in both tangible and intangible legacy.Complete tourism infrastructure,an optimistic sport and cultural atmosphere,and the improvement of residents’health concept and sports awareness have become boosters for the development of ice and snow tourism in the Beijing-Zha
文摘Beijing will host the 2022 Winter Olympics,and China strengthens research on various aspects to allow their athletes to compete successfully in winter sport.Simultaneously,Government-directed initiatives aim to increase public participation in recreational winter sport.These parallel developments allow research to advance knowledge and understanding of the physiological determinants of performance and health related to winter sport.Winter sport athletes often conduct a substan-tial amount of training with high volumes of low-to-moderate exercise intensity and lower volumes of high-intensity work.Moreover,much of the training occur at low ambient temperatures and winter sport athletes have high risk of developing asthma or asthma-related conditions,such as exercise-induced bronchoconstriction.The high training volumes require optimal nutrition with increased energy and dietary protein requirement to stimulate muscle protein synthesis response in the post-exercise period.Whether higher protein intake is required in the cold should be investigated.Cross-country ski-ing is performed mostly in Northern hemisphere with a strong cultural heritage and sporting tradition.It is expected that innovative initiatives on recruitment and training during the next few years will target to enhance performance of Chinese athletes in classical endurance-based winter sport.The innovation potential coupled with resourcing and population may be substantial with the potential for China to become a significant winter sport nation.This paper discusses the physiological aspects of endurance training and performance in winter sport highlighting areas where innovation may advance in athletic performance in cold environments.In addition,to ensure sustainable development of snow sport,a quality ski patrol and rescue system is recommended for the safety of increasing mass participation.
文摘Wearable devices have been used in the treatment and rehabilitation of ankle injuries.This article systematically reviewed the trials that summarize and evaluate the effectiveness of rehabilitation treatment after an ankle injury.Three databases,PubMed(1974–2021),Embase,and Web of Science(1950–2021),were searched.The intervention was any wearable device,and the outcome measures were Activities Scale for Kids performance(ASKp),Foot and Ankle Outcome Score(FAOS),American Orthopaedic Foot and Ankle Society(AOFAS),Olerud-Molander Ankle Score(OMAS),and Circumference as measured by any validated outcome measure.Two independent authors evaluated the studies with the Cochrane risk-of-bias tool.Four papers were included,involving 476 participants,with a mean age of 29.3±6.7 years.The mean duration of wearable devices was 3.83 weeks,and the mean length of training was 3.75 weeks.Wearable devices achieved better results compared with control on the functional performance(standardized mean difference[SMD]0.66;95%confidence interval[CI]0.29 to 1.04;I^(2)=76%;P<0.001),as well as ankle score(SMD 0.78;95%CI 0.22 to 1.35;I^(2)=82%;P<0.001).The definitive judgment could not be made due to the variability in training,training duration,and outcomes measurement.Wearable devices are a promising approach that has positive effects on ankle injuries in terms of functional performance and reducing the extent of swelling.There is insufficient evidence from randomized controlled trials(RCTs)to support this for ankle injury patients using wearable devices.Therefore,there is an need for well-conducted randomized controlled trials investigating more adaptive orthoses to achieve more effective strategies for early functional rehabilitation.PROSPERO registration number:CRD42021246289.
文摘The effects of Greyhound racing on plasma insulin, glucagon and non-esterified fatty acid have not been extensively studied. We sought to examine the effects of exercise in 19 greyhounds trialed over 400 meters on serum biochemistry, osmolality, nonessential fatty acids, and the major glucoregulatory hormones insulin and glucagon, before and after racing. Due to the non-traditional dietary patterns of many racing Greyhounds, the diet was analyzed for macro and micro-nutrient assessment to determine adequacy of the diet. Many significant biochemical changes were observed and agreed with previous studies, except for low resting glucose, low post-exercise globulin and phosphorus concentrations, and a high CK both before and after exercise. Insulin concentrations increased 10 minutes post-exercise which might be a result of the rise in serum glucose observed. Non-esterified fatty acids decreased 10 minutes post-exercise, as a likely result of the insulin facilitated cellular uptake of fatty acids early in recovery. Glucagon concentrations did not change after exercise despite the rise in insulin. The diet analysis found some mineral deficiencies in sodium, selenium, iodine and copper;and contained a far higher protein and fat content, being atypical of diets that have been shown to improve the performance in racing Greyhounds.
基金Major Science and Technology Projects of Hainan Province(ZDKJ2019009)Research Project of Ji’nan Microecological Biomedicine Shandong Labora-tory(JNL-2022002A and JNL-2022023C)+3 种基金Public Projects of Zhe-jiang Province(LGF21H030006)Research Unit Project of Chinese Academy of Medical Sciences(2019-I2M-5-030)the National Natu-ral Science Foundation of China(81721091,62073211)the Na-tional S&T Major Project for Infectious Diseases(2017ZX10203205).
文摘Introduction Organ transplantation increases survival and improves qual-ity of life to many patients with end-stage organ failure.Or-gan shortage is a worldwide problem that restricts organ trans-plantation[1].Organ procurement and preservation as well as ischemia-reperfusion injury(IRI)after transplantation are the im-portant factors affecting prognosis of recipients.Since the de-velopment of organ transplantation technology in the 20th cen-tury,organ protection technology has been a most promising con-cept in this field.Organ preservation solutions such as the Collins solution,University of Wisconsin(UW)solution,and histidine-tryptophan-ketoglutarate(HTK)solution were developed sequen-tially[2],which developed rapidly in static cold storage(SCS)tech-niques.SCS remains the standard preservation technique for organ transplantation[2].
