Aim To investigate the effects of Helicobacter pylori(Hp) infection and CagA gene on the gastric epithelial cell proliferation and apoptosis, and investigate the mechanisms of Hp increasing the risk of development of ...Aim To investigate the effects of Helicobacter pylori(Hp) infection and CagA gene on the gastric epithelial cell proliferation and apoptosis, and investigate the mechanisms of Hp increasing the risk of development of gastric cancer.Methods Endoscopic gastric mucosal biopsies were taken from 127 patients(chronic superficial gastritis,CSG; chronic atrophic gastritis,CAG; chronic atrophic gastritis with intestinal metaplasia,CAGIM; dysplasia,DYS; gastric cancer,GC) and 14 normal subjects(NS).The gastric antral epithelial cell proliferation was evaluated by ki-67 immunohistochemical technique, apoptosis cells in the gastric mucosa were quantitated after terminal deoxynucleotidyl transferase mediated dUTP nick end-labelling and Hp CagA gene was detected by polymerase chain reaction(PCR).Results Ki-67 labelling index(LI) and apoptosis index(AI) of Hp positive patients were significantly higher than that of Hp negative patients or normal controls( P <0.05 and P<0.01) .The LI and AI in Hp positive CSG patients were significantly higher than that in Hp negative CSG patients ( P<0.01); there was no significant difference between the patients with Hp infection and without Hp infection of the other four groups.Patients infected with CagA + Hp had significantly higher LI and much lower AI than that infected with CagA - Hp( P<0.05 ).The AI and LI had a positive correlation in Hp positive or negative CSG group and NS group, and a negative correlation in GC group.There was no correlation between LI or AI and the severity of gastritis.Conclusions Hp induced gastric epithelial proliferation and apoptosis predominantly in the early stage of Hp infection.CagA +Hp and CagA - Hp had different ability of inducing proliferation and apoptosis.Hp infection might lead to the imbalance of the AI/LI ratio and ultimately promote the development of gastric cancer.展开更多
目的通过丙泊酚及其复合3种不同阿片类药物在无痛胃镜麻醉中效果及满意度的对比研究,探讨更安全有效的麻醉用药方案。方法选择2018年11月至2019年2月期间行无痛胃镜检查的美国麻醉医师协会(American Society of Anesthesiologists,ASA)...目的通过丙泊酚及其复合3种不同阿片类药物在无痛胃镜麻醉中效果及满意度的对比研究,探讨更安全有效的麻醉用药方案。方法选择2018年11月至2019年2月期间行无痛胃镜检查的美国麻醉医师协会(American Society of Anesthesiologists,ASA)分级Ⅰ~Ⅱ级门急诊患者385例,利用随机数字法将患者分成四组:丙泊酚复合0.9%氯化钠溶液组(A组,97例)、丙泊酚复合芬太尼组(B组,95例)、丙泊酚复合舒芬太尼组(C组,98例)、丙泊酚复合瑞芬太尼组(D组,95例),比较四组患者的术前、术中不同时间点生命体征变化、苏醒时间、离院时间、丙泊酚总用量、患者和手术者对麻醉的满意度、不良反应发生率。结果①生命体征变化:四组患者组间比较,经皮动脉血氧饱和度[(percutaneous arterial oxygen saturation)以SpO2表示]在胃镜检查开始即刻(T1)D组显著低于其他三组,差异有统计学意义(P<0.05)。四组患者组内比较平均血压(mean blood pressure,MBP)、心率、SpO2均在T1时间点出现下降,差异有统计学意义(P<0.05)。②苏醒时间、离院时间及丙泊酚用量比较:A组苏醒时间、离院时间均明显长于B、C、D组(P<0.05),C组离院时间明显短于B、D组(P<0.05);A组丙泊酚用量最大,D次之,A和B、C、D组分别比较差异有统计学意义(P<0.05),D组分别与B、C组比较,差异均有统计学意义(P<0.05)。③患者满意度比较:A组满意度较低,分别同B、C、D组比较,差异均有统计学意义(P<0.05)。C组满意度评分最高,分别与B、D组比较,差异有统计学意义(P<0.05)。④手术医师满意度比较:C组手术医师满意度较高,A组和C组间差异有统计学意义(P<0.05),其余各组之间两两比较差异无统计学意义。⑤不良反应发生率比较:A组不良反应中低血压发生率较高,与其余三组相比差异有统计学意义(P<0.05)。结论结合术中生命体征变化、苏醒时间、离院时间、术中不良反应发生率、患者展开更多
文摘Aim To investigate the effects of Helicobacter pylori(Hp) infection and CagA gene on the gastric epithelial cell proliferation and apoptosis, and investigate the mechanisms of Hp increasing the risk of development of gastric cancer.Methods Endoscopic gastric mucosal biopsies were taken from 127 patients(chronic superficial gastritis,CSG; chronic atrophic gastritis,CAG; chronic atrophic gastritis with intestinal metaplasia,CAGIM; dysplasia,DYS; gastric cancer,GC) and 14 normal subjects(NS).The gastric antral epithelial cell proliferation was evaluated by ki-67 immunohistochemical technique, apoptosis cells in the gastric mucosa were quantitated after terminal deoxynucleotidyl transferase mediated dUTP nick end-labelling and Hp CagA gene was detected by polymerase chain reaction(PCR).Results Ki-67 labelling index(LI) and apoptosis index(AI) of Hp positive patients were significantly higher than that of Hp negative patients or normal controls( P <0.05 and P<0.01) .The LI and AI in Hp positive CSG patients were significantly higher than that in Hp negative CSG patients ( P<0.01); there was no significant difference between the patients with Hp infection and without Hp infection of the other four groups.Patients infected with CagA + Hp had significantly higher LI and much lower AI than that infected with CagA - Hp( P<0.05 ).The AI and LI had a positive correlation in Hp positive or negative CSG group and NS group, and a negative correlation in GC group.There was no correlation between LI or AI and the severity of gastritis.Conclusions Hp induced gastric epithelial proliferation and apoptosis predominantly in the early stage of Hp infection.CagA +Hp and CagA - Hp had different ability of inducing proliferation and apoptosis.Hp infection might lead to the imbalance of the AI/LI ratio and ultimately promote the development of gastric cancer.
文摘目的通过丙泊酚及其复合3种不同阿片类药物在无痛胃镜麻醉中效果及满意度的对比研究,探讨更安全有效的麻醉用药方案。方法选择2018年11月至2019年2月期间行无痛胃镜检查的美国麻醉医师协会(American Society of Anesthesiologists,ASA)分级Ⅰ~Ⅱ级门急诊患者385例,利用随机数字法将患者分成四组:丙泊酚复合0.9%氯化钠溶液组(A组,97例)、丙泊酚复合芬太尼组(B组,95例)、丙泊酚复合舒芬太尼组(C组,98例)、丙泊酚复合瑞芬太尼组(D组,95例),比较四组患者的术前、术中不同时间点生命体征变化、苏醒时间、离院时间、丙泊酚总用量、患者和手术者对麻醉的满意度、不良反应发生率。结果①生命体征变化:四组患者组间比较,经皮动脉血氧饱和度[(percutaneous arterial oxygen saturation)以SpO2表示]在胃镜检查开始即刻(T1)D组显著低于其他三组,差异有统计学意义(P<0.05)。四组患者组内比较平均血压(mean blood pressure,MBP)、心率、SpO2均在T1时间点出现下降,差异有统计学意义(P<0.05)。②苏醒时间、离院时间及丙泊酚用量比较:A组苏醒时间、离院时间均明显长于B、C、D组(P<0.05),C组离院时间明显短于B、D组(P<0.05);A组丙泊酚用量最大,D次之,A和B、C、D组分别比较差异有统计学意义(P<0.05),D组分别与B、C组比较,差异均有统计学意义(P<0.05)。③患者满意度比较:A组满意度较低,分别同B、C、D组比较,差异均有统计学意义(P<0.05)。C组满意度评分最高,分别与B、D组比较,差异有统计学意义(P<0.05)。④手术医师满意度比较:C组手术医师满意度较高,A组和C组间差异有统计学意义(P<0.05),其余各组之间两两比较差异无统计学意义。⑤不良反应发生率比较:A组不良反应中低血压发生率较高,与其余三组相比差异有统计学意义(P<0.05)。结论结合术中生命体征变化、苏醒时间、离院时间、术中不良反应发生率、患者
