应用蛋白质组学双向凝胶电泳(Two-dimensional gel electrophoresis,2DE)和质谱技术,定量分析和鉴定了癫痫组(n=3)和正常组(n=3)脑组织的差异表达蛋白,以从蛋白质水平上揭示癫痫病的发生机制.结果表明,凝胶图谱可辨识2500～3000个蛋白点...应用蛋白质组学双向凝胶电泳(Two-dimensional gel electrophoresis,2DE)和质谱技术,定量分析和鉴定了癫痫组(n=3)和正常组(n=3)脑组织的差异表达蛋白,以从蛋白质水平上揭示癫痫病的发生机制.结果表明,凝胶图谱可辨识2500～3000个蛋白点,对21个显著差异表达蛋白点进行质谱鉴定和SwissProt数据库检索,得到17个癫痫差异蛋白,其中2个蛋白在癫痫组织中表达上调,15个蛋白表达下调.部分蛋白与癫痫的关系属首次报道.这些蛋白与癫痫的病变相关,可能成为癫痫的分子标志物和药物治疗的靶向蛋白.展开更多
10 temple courtyards and 10 temple gardens were chosen as the research object in Nanjing.There were 146 species of vascular plants(including variety),which belong to 96 genera and 63 families.The Richness index,Simpso...10 temple courtyards and 10 temple gardens were chosen as the research object in Nanjing.There were 146 species of vascular plants(including variety),which belong to 96 genera and 63 families.The Richness index,Simpson index,Species diversity and Similarity index of the plant community were analyzed.The results showed:①The dominant species of temple courtyards and temple gardens were all trees;the density of shrub layer(0.32 strains/m2) was greater than that of the tree layer(0.21 strains/m2).And the density of tree layer(2.4 strains/m2) and shrub layer(4.5 strains/m2) in temple gardens were totally higher than those of temple courtyards.②Both the Simpson index and the Species diversity in temple garden plots were higher than those of the temple courtyard plots;The Pielou index was at a higher level both in tree layer and shrub layer,which indicated that quantities of each tree species were on an average level.③The Similarity index in temple gardens was higher than that of the temple courtyards,both less than 50%,which indicated that the repetition rate of each tree species was obviously greater in temple gardens than in temple courtyards.展开更多
文摘应用蛋白质组学双向凝胶电泳(Two-dimensional gel electrophoresis,2DE)和质谱技术,定量分析和鉴定了癫痫组(n=3)和正常组(n=3)脑组织的差异表达蛋白,以从蛋白质水平上揭示癫痫病的发生机制.结果表明,凝胶图谱可辨识2500～3000个蛋白点,对21个显著差异表达蛋白点进行质谱鉴定和SwissProt数据库检索,得到17个癫痫差异蛋白,其中2个蛋白在癫痫组织中表达上调,15个蛋白表达下调.部分蛋白与癫痫的关系属首次报道.这些蛋白与癫痫的病变相关,可能成为癫痫的分子标志物和药物治疗的靶向蛋白.
文摘10 temple courtyards and 10 temple gardens were chosen as the research object in Nanjing.There were 146 species of vascular plants(including variety),which belong to 96 genera and 63 families.The Richness index,Simpson index,Species diversity and Similarity index of the plant community were analyzed.The results showed:①The dominant species of temple courtyards and temple gardens were all trees;the density of shrub layer(0.32 strains/m2) was greater than that of the tree layer(0.21 strains/m2).And the density of tree layer(2.4 strains/m2) and shrub layer(4.5 strains/m2) in temple gardens were totally higher than those of temple courtyards.②Both the Simpson index and the Species diversity in temple garden plots were higher than those of the temple courtyard plots;The Pielou index was at a higher level both in tree layer and shrub layer,which indicated that quantities of each tree species were on an average level.③The Similarity index in temple gardens was higher than that of the temple courtyards,both less than 50%,which indicated that the repetition rate of each tree species was obviously greater in temple gardens than in temple courtyards.
