It thas been reported that the various experimental liver injuries and patients with liver diseases are accompanied by intestinal endotoxemia (IETM). Up to now, the effect of IETM on the chronicity of hepatitis, its p...It thas been reported that the various experimental liver injuries and patients with liver diseases are accompanied by intestinal endotoxemia (IETM). Up to now, the effect of IETM on the chronicity of hepatitis, its pervention and treatment have received little attention.According to the study of a large number of animal experiments and clinics, we raise a new conception of the pathogenesis of hepatic injury in hepatitis:The various factors induce liver injury(for example,hepatic virus, alcohol, medicines and hepatotoxic agents and so on )by various specific mechanisms,which is called "The primary liver injury ". IETM is occurred in the deveopment of hepatitis. Endotoxin activated kupffer cells and produced a number of chemical mediators. The liver injury caused by mediators is called"The secondary liver injury". This "The secondary liver injury" depends on serious degree of IETM. The repeated and persistent hepatocellular necrosis in light IETM condition accompanying hepatocellular regeneration and inflammatory cells infiltration develop the chronic hepatitis and/or liver fibrosis, even cirrhosis and hepatocarcinoma. The severe IETM offen leads to overinflammation following serious hepatocellular necrosis, even grows up severe hepatitis, liver failure further is appeared.展开更多
文摘目的探讨黄酮类化合物芹菜素对大鼠实验性心肌缺血/再灌注(ischemia/reperfusion,I/R)时心肌细胞凋亡与Bcl-2、Bax、Caspase-3蛋白表达的影响,并分析心肌组织病理学损伤程度。方法采用结扎左冠状动脉前降支,心肌缺血45 min,再灌注2 h制作缺血/再灌注模型。将大鼠随机分为8组,即正常组(normal group,Normal)、假手术组(sham oper-ation group,Sham)、生理盐水缺血/再灌注组(saline ischemi-a-reperfusion group,NS)、溶剂对照组(solvent control group,Sol)、美托洛尔对照组(metoprolol control group,Meto)、芹菜素低、中、高剂量(1、2、4 mg.kg-1)用药组(apigenin low,medium and high dose treatment group,Api1,Api2,Api4)。再灌注2 h后迅速取出心脏,TUNEL法原位标记凋亡的心肌细胞;免疫组化法测Bcl-2、Bax和Caspase-3蛋白表达;做病理组织切片检查心肌损伤情况。结果芹菜素各剂量组心肌细胞凋亡率明显低于NS组(P<0.05),Api1,Api2,Api4能剂量依赖性地降低大鼠缺血/再灌注心肌细胞凋亡率;芹菜素各剂量组剂量依赖性地提高大鼠心肌缺血/再灌注的Bcl-2蛋白表达量(P<0.05)、降低大鼠心肌缺血/再灌注的Bax、Caspase-3蛋白表达量(P<0.05);芹菜素各剂量组与NS组比较,心肌组织损伤的病理学变化明显减轻(P<0.05)。结论芹菜素对缺血/再灌注心肌的保护效应可能与其抑制缺血/再灌注心肌细胞凋亡有关;芹菜素抗心肌凋亡作用的机制可能与其上调Bcl-2蛋白表达和下调Bax、Caspase-3蛋白表达有关;芹菜素能明显减轻心肌组织损伤。
文摘It thas been reported that the various experimental liver injuries and patients with liver diseases are accompanied by intestinal endotoxemia (IETM). Up to now, the effect of IETM on the chronicity of hepatitis, its pervention and treatment have received little attention.According to the study of a large number of animal experiments and clinics, we raise a new conception of the pathogenesis of hepatic injury in hepatitis:The various factors induce liver injury(for example,hepatic virus, alcohol, medicines and hepatotoxic agents and so on )by various specific mechanisms,which is called "The primary liver injury ". IETM is occurred in the deveopment of hepatitis. Endotoxin activated kupffer cells and produced a number of chemical mediators. The liver injury caused by mediators is called"The secondary liver injury". This "The secondary liver injury" depends on serious degree of IETM. The repeated and persistent hepatocellular necrosis in light IETM condition accompanying hepatocellular regeneration and inflammatory cells infiltration develop the chronic hepatitis and/or liver fibrosis, even cirrhosis and hepatocarcinoma. The severe IETM offen leads to overinflammation following serious hepatocellular necrosis, even grows up severe hepatitis, liver failure further is appeared.
