目的比较测试片法(test piece method,TP)、计数琼脂平板法(counting Agar plate method,CAP)及琼脂倾注TTC平板法(2,3,5-chloride three phenyl tetrazole,TTC)对食品微生物菌落总数的测定效果。方法选取70例需进行菌落总数测定的食品...目的比较测试片法(test piece method,TP)、计数琼脂平板法(counting Agar plate method,CAP)及琼脂倾注TTC平板法(2,3,5-chloride three phenyl tetrazole,TTC)对食品微生物菌落总数的测定效果。方法选取70例需进行菌落总数测定的食品样本作为研究对象,分别应用测试片法、计数琼脂平板法及琼脂平板法进行测定,对比三种方法的菌落总数超标样品检出率。结果 TTC法菌落总数超标检出率为24.3%,显著高于测试片法(7.1%)及计数琼脂平板法(11.4%),P<0.05。结论与TP及CAP相比,TTC法对于菌落总数超标样品的检出率更高、灵敏度更好。展开更多
文摘目的比较测试片法(test piece method,TP)、计数琼脂平板法(counting Agar plate method,CAP)及琼脂倾注TTC平板法(2,3,5-chloride three phenyl tetrazole,TTC)对食品微生物菌落总数的测定效果。方法选取70例需进行菌落总数测定的食品样本作为研究对象,分别应用测试片法、计数琼脂平板法及琼脂平板法进行测定,对比三种方法的菌落总数超标样品检出率。结果 TTC法菌落总数超标检出率为24.3%,显著高于测试片法(7.1%)及计数琼脂平板法(11.4%),P<0.05。结论与TP及CAP相比,TTC法对于菌落总数超标样品的检出率更高、灵敏度更好。
基金Supported by the China-U.S.Collaborative Program on Emerging and Re-emerging Infectious Diseases(No.1U2GGH000018-01)the National Basic Research Program of China(973Program)(No.2010CB530206)the National Science and Technology Key Project on Major Infectious Disease(Nos.2008ZX10004-002,2008ZX10004-008,2009ZX10004-203,2012ZX10004-215)~~
