Objective： To investigate the relationship between the excitotoxicity and seruminducible kinase （SNK） and spine-associated Rap GTPase-activating protein （SPAR） pathway in primary hippocampal neuron injury induced...Objective： To investigate the relationship between the excitotoxicity and seruminducible kinase （SNK） and spine-associated Rap GTPase-activating protein （SPAR） pathway in primary hippocampal neuron injury induced by glutamate and furthermore, to explore the molecular mechanism of neuroprotection of Zibu Piyin Recipe （滋补脾阴方药, ZBPYR） and the relationship between ZBPYR and the morphological regulation of dendritic spines. Methods： The serum containing ZBPYR was prepared by seropharmacology. Reverse transcription and polymerase chain reaction （RT-PCR） was used to detect the expression of mRNA for SNK, SPAR, postsynaptic density protein 95 （PSD-95） and N-methyI-D-aspartate （NMDA） receptor subunits （NR1, NR2A and NR2B） in primary rat hippocampal neuron cultures after pretreatment with 10 μmol/L glutamate and ZBPYR serum. Results： ZBPYR serum pretreatment resulted in a significant down-regulation of glutamate-induced SNK mRNA expression （P〈0.05）. Significant up-regulation was seen on the mRNA expression of SPAR and PSD-95 （P〈0.05）. All these changes were dose-dependent. The mRNA expression of NR1, NR2A and NR2B was down-regulated to different degrees （P〈0.05）. Conclusion： The mechanism of effect of ZBPYR on glutamate-induced excitotoxicity may be related to the regulation of SNK-SPAR signal pathway. ZBPYR may play a role in protecting and maintaining the normal morphology and structure of dendritic spines, which may be achieved by inhibiting the excessive activation of NMDA receptors.展开更多
Objective:To investigate effects of Zibu Shenjing Fang(滋补肾精方) on growth and development of the mouse with insufficiency of kidney-essence and the mechanism.Methods:Total 50 mice were randomly divided into a norma...Objective:To investigate effects of Zibu Shenjing Fang(滋补肾精方) on growth and development of the mouse with insufficiency of kidney-essence and the mechanism.Methods:Total 50 mice were randomly divided into a normal group,a model group,a Jingui Shenqi Wan(金匮肾气丸) group,a Zibu Shenjing Fang high dose group and a Zibu Shenjing Fang low dose group,10 mice in each group.The kidney-essence insufficiency mouse model was established by use of threat-injuring the kidney combined with over-fatigue.At the same time of modeling,the mice in the model group were intragastrically administrated with saline 20 mL·kg-1·d-1,in the Jingui Shenqi Wan group with suspension of the Jingui Shenqi Wan 2.7 g·kg-1·d-1,in the Zibu Shenjing Fang high dose group with Zibu Shenjing Fang 20 g·kg-1·d-1 and in the Zibu Shenjing Fang low dose group with Zibu Shenjing Fang 10 g·kg-1·d-1,for 21 consecutive days.The general state was observed,the body weight was weighted,and serum growth hormone(GH) and insulin-like growth factor-1(IGF-1) contents were detected.Results:Compared with model group,Zibu Shenjing Fang groups and Jingui Shenqi Wan group could improve manifestation of the mouse with kidney-essence insufficiency,increase body weight of the mouse and serum GH and IGF-1 contents,especially in the high dose group.Conclusion:Zibu Shenjing Fang gives play to the function of tonifying the kidney and replenishing essence through regulating GH and IGF-1 levels,so as to influence growth and development of the mouse.展开更多
基金the National Natural Science Foundation of China(No.30472255)
文摘Objective： To investigate the relationship between the excitotoxicity and seruminducible kinase （SNK） and spine-associated Rap GTPase-activating protein （SPAR） pathway in primary hippocampal neuron injury induced by glutamate and furthermore, to explore the molecular mechanism of neuroprotection of Zibu Piyin Recipe （滋补脾阴方药, ZBPYR） and the relationship between ZBPYR and the morphological regulation of dendritic spines. Methods： The serum containing ZBPYR was prepared by seropharmacology. Reverse transcription and polymerase chain reaction （RT-PCR） was used to detect the expression of mRNA for SNK, SPAR, postsynaptic density protein 95 （PSD-95） and N-methyI-D-aspartate （NMDA） receptor subunits （NR1, NR2A and NR2B） in primary rat hippocampal neuron cultures after pretreatment with 10 μmol/L glutamate and ZBPYR serum. Results： ZBPYR serum pretreatment resulted in a significant down-regulation of glutamate-induced SNK mRNA expression （P〈0.05）. Significant up-regulation was seen on the mRNA expression of SPAR and PSD-95 （P〈0.05）. All these changes were dose-dependent. The mRNA expression of NR1, NR2A and NR2B was down-regulated to different degrees （P〈0.05）. Conclusion： The mechanism of effect of ZBPYR on glutamate-induced excitotoxicity may be related to the regulation of SNK-SPAR signal pathway. ZBPYR may play a role in protecting and maintaining the normal morphology and structure of dendritic spines, which may be achieved by inhibiting the excessive activation of NMDA receptors.
文摘Objective:To investigate effects of Zibu Shenjing Fang(滋补肾精方) on growth and development of the mouse with insufficiency of kidney-essence and the mechanism.Methods:Total 50 mice were randomly divided into a normal group,a model group,a Jingui Shenqi Wan(金匮肾气丸) group,a Zibu Shenjing Fang high dose group and a Zibu Shenjing Fang low dose group,10 mice in each group.The kidney-essence insufficiency mouse model was established by use of threat-injuring the kidney combined with over-fatigue.At the same time of modeling,the mice in the model group were intragastrically administrated with saline 20 mL·kg-1·d-1,in the Jingui Shenqi Wan group with suspension of the Jingui Shenqi Wan 2.7 g·kg-1·d-1,in the Zibu Shenjing Fang high dose group with Zibu Shenjing Fang 20 g·kg-1·d-1 and in the Zibu Shenjing Fang low dose group with Zibu Shenjing Fang 10 g·kg-1·d-1,for 21 consecutive days.The general state was observed,the body weight was weighted,and serum growth hormone(GH) and insulin-like growth factor-1(IGF-1) contents were detected.Results:Compared with model group,Zibu Shenjing Fang groups and Jingui Shenqi Wan group could improve manifestation of the mouse with kidney-essence insufficiency,increase body weight of the mouse and serum GH and IGF-1 contents,especially in the high dose group.Conclusion:Zibu Shenjing Fang gives play to the function of tonifying the kidney and replenishing essence through regulating GH and IGF-1 levels,so as to influence growth and development of the mouse.
