Objective:This study was aimed at assessing the dynamics of vitronectin (VN), laminin (LN), and heparan sulfate/heparin (HS/HP) content changes during experimental burn healing. Methods:VN, LN, and HS/HP were isolated...Objective:This study was aimed at assessing the dynamics of vitronectin (VN), laminin (LN), and heparan sulfate/heparin (HS/HP) content changes during experimental burn healing. Methods:VN, LN, and HS/HP were isolated and purified from normal and injured skin of domestic pigs, on the 3rd, 5th, 10th, 15th, and 21st days following thermal damage. The wounds were treated with apitherapeutic agent (propolis), silver sulfadiazine (SSD), physiological salt solution, and propolis vehicle. VN and LN were quantified using an immunoenzymatic assay and HS/HP was estimated by densitometric analysis. Results:Propolis treatment stimulated significant increases in VN, LN, and HS/HP contents during the initial phase of study, followed by a reduction in the estimated extracellular matrix molecules. Similar patterns, although less extreme, were observed after treatment with SSD. Conclusions:The beneficial effects of propolis on experimental wounds make it a potential apitherapeutic agent in topical burn management.展开更多
Biomaterial surface chemistry engenders profound consequences on cell adhesion and the ultimate tissue response by adsorbing proteins from extracellular matrix,where vitronectin(Vn)is involved as one of the crucial me...Biomaterial surface chemistry engenders profound consequences on cell adhesion and the ultimate tissue response by adsorbing proteins from extracellular matrix,where vitronectin(Vn)is involved as one of the crucial mediator proteins.Deciphering the adsorption behaviors of Vn in molecular scale provides a useful account of how to design biomaterial surfaces.But the details of structural dynamics and consequential biological effect remain elusive.Herein,both experimental and computational approaches were applied to delineate the conformational and orientational evolution of Vn during adsorption onto self-assembled monolayers(SAMs)terminating with-COOH,-NH2,-CH3 and-OH.To unravel the interplay between cell binding and the charge and wettability of material surface,somatomedin-B(SMB)domain of Vn holding the RGD cell-binding motif was employed in molecular dynamics(MD)simulations,with orientation initialized by Monte Carlo(MC)method.Experimental evidences including protein adsorption,cell adhesion and integrin gene expressions were thoroughly investigated.The adsorption of Vn on different surface chemistries showed very complex profiles.Cell adhesion was enabled on all Vn-adsorbed surfaces but with distinct mechanisms mostly determined by conformational change induced reorientation.Higher amount of Vn was observed on negatively charged surface(COOH)and hydrophobic surface(CH3).However,advantageous orientations defined by RGD loop conditions were only obtained on the charged surfaces(COOH and NH2).Specifically,COOH surface straightened up the Vn molecules and accumulated them into a higher density,whereas CH3 surface squashed Vn and stacked them into higher density multilayer by tracking adsorption but with the RGD loops restrained.These findings may have a broad implication on the understanding of Vn functionality and would help develop new strategies for designing advanced biomaterials.展开更多
文摘目的 探讨不同严重程度冠状动脉疾病患者外周血中超敏C反应蛋白(hs-CRP)、丙二醛(MDA)、玻连蛋白(VN)、同型半胱氨酸(Hcy)的水平差异。方法 选取冠状动脉疾病患者90例,分为稳定型心绞痛组(SA组)30例,不稳定型心绞痛组(UA组)30例,急性心肌梗死组(AMI组)30例。另随机选取同期30例入院进行查体者作为对照组。检测所有研究对象外周血中hs-CRP、VN、MDA和Hcy的水平。结果 各型冠状动脉疾病患者外周血中hs-CRP、VN、MDA和Hcy水平均值均高于对照组,差异均有统计学意义( P <0.05)。而且随着疾病严重程度的加重,SA组、UA组、AMI组患者血清hs-CRP、VN、MDA和Hcy表达水平依次升高,差异均有统计学意义( P <0.05)。结论 hs-CRP、VN、MDA和Hcy可能参与动脉粥样硬化的进程,可作为冠状动脉疾病患者诊断和预测的生化标志物。
基金Project (KNW-2-138/09) supported by the Medical University of Silesia, Poland
文摘Objective:This study was aimed at assessing the dynamics of vitronectin (VN), laminin (LN), and heparan sulfate/heparin (HS/HP) content changes during experimental burn healing. Methods:VN, LN, and HS/HP were isolated and purified from normal and injured skin of domestic pigs, on the 3rd, 5th, 10th, 15th, and 21st days following thermal damage. The wounds were treated with apitherapeutic agent (propolis), silver sulfadiazine (SSD), physiological salt solution, and propolis vehicle. VN and LN were quantified using an immunoenzymatic assay and HS/HP was estimated by densitometric analysis. Results:Propolis treatment stimulated significant increases in VN, LN, and HS/HP contents during the initial phase of study, followed by a reduction in the estimated extracellular matrix molecules. Similar patterns, although less extreme, were observed after treatment with SSD. Conclusions:The beneficial effects of propolis on experimental wounds make it a potential apitherapeutic agent in topical burn management.
基金financially supported by the National Key R&D Program of China(2017YFC1105000)Science and Technology Planning Project of Guangdong Province(2017B030314008)+5 种基金National Natural Science Foundation of China(51572087,31700823)Shenzhen Science and Technology Innovation Committee(JCYJ20170818160503855)Outstanding Scholar Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory(2018GZR110102001)GDST-NWO science industry cooperation programme Chemistry(2018A050501006)Natural Science Foundation of Guangdong Province of China(2020A1515011354)the 111 Project(B13039).
文摘Biomaterial surface chemistry engenders profound consequences on cell adhesion and the ultimate tissue response by adsorbing proteins from extracellular matrix,where vitronectin(Vn)is involved as one of the crucial mediator proteins.Deciphering the adsorption behaviors of Vn in molecular scale provides a useful account of how to design biomaterial surfaces.But the details of structural dynamics and consequential biological effect remain elusive.Herein,both experimental and computational approaches were applied to delineate the conformational and orientational evolution of Vn during adsorption onto self-assembled monolayers(SAMs)terminating with-COOH,-NH2,-CH3 and-OH.To unravel the interplay between cell binding and the charge and wettability of material surface,somatomedin-B(SMB)domain of Vn holding the RGD cell-binding motif was employed in molecular dynamics(MD)simulations,with orientation initialized by Monte Carlo(MC)method.Experimental evidences including protein adsorption,cell adhesion and integrin gene expressions were thoroughly investigated.The adsorption of Vn on different surface chemistries showed very complex profiles.Cell adhesion was enabled on all Vn-adsorbed surfaces but with distinct mechanisms mostly determined by conformational change induced reorientation.Higher amount of Vn was observed on negatively charged surface(COOH)and hydrophobic surface(CH3).However,advantageous orientations defined by RGD loop conditions were only obtained on the charged surfaces(COOH and NH2).Specifically,COOH surface straightened up the Vn molecules and accumulated them into a higher density,whereas CH3 surface squashed Vn and stacked them into higher density multilayer by tracking adsorption but with the RGD loops restrained.These findings may have a broad implication on the understanding of Vn functionality and would help develop new strategies for designing advanced biomaterials.
