Objective： To study the effect of salvianolic acid A （SAA） on L-type calcium current （I-CaL） in isolated ventdcular myocytes of Sprague-Dawley rats. Methods： SPA powder was dissolved in normal Tyrode＇s solution...Objective： To study the effect of salvianolic acid A （SAA） on L-type calcium current （I-CaL） in isolated ventdcular myocytes of Sprague-Dawley rats. Methods： SPA powder was dissolved in normal Tyrode＇s solution to reach the concentrations of 1, 10, 100, and 1000 μmol/L. The traditional whole-cell patch-clamp recording technique was employed to evaluate the effects of SAA on I-CaL in single ventricular myocytes which were prepared by Langendorff perfusion apparatus from Sprague-Dawley rats. Results： SPA （1, 10, 100, and 1000 μmol/L） inhibited I-CaL peak value by 16.23%± 1.3% （n=6, P〈0.05）, 22.9% ± 3.6% （n=6, P〈0.05）, 53.4% ± 3.0% （n=8, P〈0.01）, and 62.26% ± 2.9% （n=8, P〈0.01）, respectively. SAA reversibly inhibited I-CaL in a dose-dependent manner and with a half-blocking concentration （IC50） of 38.3 μmol/L. SAA at 100 μmol/L elevated the I-V curve obviously, and shifted the half-active voltage （V0.5） from （-15.78± 0.86） mV to （-11.24 ± 0.77） mV （n=6, P〈0.05） and the slope （K） from 5.33 ±0.74 to 4.35±0.74 （n=6, P〉0.05）. However, it did not alter the shapes of I-V curve, steady-state inactivation curve, or recovery from inactivation curve. Conclusions： SAA inhibited I-CaL in a dose-dependent manner. It shifted the steady-state activation curve to a more positive voltage, which indicated that the drug affected the activated state of calcium channels, and suggested that the Ca2. antagonistic effect of SPA be beneficial in the treatment of myocardial ischemia reperfusion injury.展开更多
Objectire This study compares the effects of small dose of recombinant tissue - type plasminogenactivator (tPA) with those of conventional dose of urokinase (UK) and assesses the influence of different modes ofintrave...Objectire This study compares the effects of small dose of recombinant tissue - type plasminogenactivator (tPA) with those of conventional dose of urokinase (UK) and assesses the influence of different modes ofintravenous UK administration in patients with acute myocardial infarction (AMI). Methods Eighty patientswith AMI were randomized to 50mg of tPA (Group Ⅰ, n=26) using an accelerating approach or to 1.0-1.5 millionU of UK (Group Ⅱ, n=54). UK was administered as a single bolus injection of whole dose (GrouP Ⅱa, n=26) orhalf dose bolus injection followed by half dose infusion (Group Ⅱb, n=28). All patients underwent coronaryartsriogrophy 90min after the initiation of intravenous thrombolysis, and the infarct - related coronary artery (IRA)patency was evaluated. Cardiac events during hospitalization were recorded and predischarge left ventricularfunction was determined by two - dimensional echocardiography. Results The IRA patency rate was significantlyhigher in Group Ⅰ (88.4%) than in Group Ⅱ (53.7%) (P<0.01). Group Ⅰ patients had less cardiac events duringhospitalization (11.5% vs 33.3%) and greater improvement in left ventricular function than group Ⅱ patients.However, these angiogrophic, left ventricular functional and prognostic parameters did not significantly differbetween Group Ⅱa and Group Ⅱb. Conclusion Thrombolysis after AMI with small dose of intravenous tPAexerts better angiographic and clinical effects than that with conventional dose of UK. The thrombolytic effects ofUK were not affected by different modes of intravenous administration of the agent.展开更多
目的探讨起源于主肺动脉干(MSPA)的室性早搏/室性心动过速(室早/室速)的电生理特征、标测方法和导管消融。方法 27例疑似右心室流出道室早/室速的患者中4例(15%)起源于MSPA,其中男性3例,平均年龄(25±10)岁。3例使用非接触式标测系...目的探讨起源于主肺动脉干(MSPA)的室性早搏/室性心动过速(室早/室速)的电生理特征、标测方法和导管消融。方法 27例疑似右心室流出道室早/室速的患者中4例(15%)起源于MSPA,其中男性3例,平均年龄(25±10)岁。3例使用非接触式标测系统结合常规标测,1例采用常规标测。2例使用温控导管消融,2例采用冷盐水灌注导管消融。结果1例(N_4)超声心动图提示致心律失常性右心室心肌病,余3例未发现器质性心脏病。1例患者为室早/室速伴有晕厥,另3例仅有室早。室早/室速体表心电图表现为下壁导联 R 波振幅高、心电轴右偏和 Q_(aVL)/Q_(aVR)比值较大。非接触式标测显示最早激动点位于球囊上方较远距离,激动面积大,最早激动点至爆发点距离远。成功靶点处激动较体表 QRS 波起始提前(28±6)ms,2例记录到等大的 A 波和 V 波,3例记录到融合的尖峰或碎裂电位,局部高能量可以获得较满意的起搏标测图形。4例患者均消融成功,随访(6.5±3.0)个月,1例复发后再次消融成功。结论起源于 MSPA 的室早/室速并非少见,非接触式标测的特殊表现可快速揭示诊断,详细的激动标测和起搏标测指导的消融具有较好的临床效果。展开更多
基金Supported by the Key Project of National Science Foundation of China(No.30830118)the National Key New Drug Project (No.2009ZX09301-005 and No.2009ZX09303-003)
文摘Objective： To study the effect of salvianolic acid A （SAA） on L-type calcium current （I-CaL） in isolated ventdcular myocytes of Sprague-Dawley rats. Methods： SPA powder was dissolved in normal Tyrode＇s solution to reach the concentrations of 1, 10, 100, and 1000 μmol/L. The traditional whole-cell patch-clamp recording technique was employed to evaluate the effects of SAA on I-CaL in single ventricular myocytes which were prepared by Langendorff perfusion apparatus from Sprague-Dawley rats. Results： SPA （1, 10, 100, and 1000 μmol/L） inhibited I-CaL peak value by 16.23%± 1.3% （n=6, P〈0.05）, 22.9% ± 3.6% （n=6, P〈0.05）, 53.4% ± 3.0% （n=8, P〈0.01）, and 62.26% ± 2.9% （n=8, P〈0.01）, respectively. SAA reversibly inhibited I-CaL in a dose-dependent manner and with a half-blocking concentration （IC50） of 38.3 μmol/L. SAA at 100 μmol/L elevated the I-V curve obviously, and shifted the half-active voltage （V0.5） from （-15.78± 0.86） mV to （-11.24 ± 0.77） mV （n=6, P〈0.05） and the slope （K） from 5.33 ±0.74 to 4.35±0.74 （n=6, P〉0.05）. However, it did not alter the shapes of I-V curve, steady-state inactivation curve, or recovery from inactivation curve. Conclusions： SAA inhibited I-CaL in a dose-dependent manner. It shifted the steady-state activation curve to a more positive voltage, which indicated that the drug affected the activated state of calcium channels, and suggested that the Ca2. antagonistic effect of SPA be beneficial in the treatment of myocardial ischemia reperfusion injury.
文摘Objectire This study compares the effects of small dose of recombinant tissue - type plasminogenactivator (tPA) with those of conventional dose of urokinase (UK) and assesses the influence of different modes ofintravenous UK administration in patients with acute myocardial infarction (AMI). Methods Eighty patientswith AMI were randomized to 50mg of tPA (Group Ⅰ, n=26) using an accelerating approach or to 1.0-1.5 millionU of UK (Group Ⅱ, n=54). UK was administered as a single bolus injection of whole dose (GrouP Ⅱa, n=26) orhalf dose bolus injection followed by half dose infusion (Group Ⅱb, n=28). All patients underwent coronaryartsriogrophy 90min after the initiation of intravenous thrombolysis, and the infarct - related coronary artery (IRA)patency was evaluated. Cardiac events during hospitalization were recorded and predischarge left ventricularfunction was determined by two - dimensional echocardiography. Results The IRA patency rate was significantlyhigher in Group Ⅰ (88.4%) than in Group Ⅱ (53.7%) (P<0.01). Group Ⅰ patients had less cardiac events duringhospitalization (11.5% vs 33.3%) and greater improvement in left ventricular function than group Ⅱ patients.However, these angiogrophic, left ventricular functional and prognostic parameters did not significantly differbetween Group Ⅱa and Group Ⅱb. Conclusion Thrombolysis after AMI with small dose of intravenous tPAexerts better angiographic and clinical effects than that with conventional dose of UK. The thrombolytic effects ofUK were not affected by different modes of intravenous administration of the agent.
文摘目的探讨起源于主肺动脉干(MSPA)的室性早搏/室性心动过速(室早/室速)的电生理特征、标测方法和导管消融。方法 27例疑似右心室流出道室早/室速的患者中4例(15%)起源于MSPA,其中男性3例,平均年龄(25±10)岁。3例使用非接触式标测系统结合常规标测,1例采用常规标测。2例使用温控导管消融,2例采用冷盐水灌注导管消融。结果1例(N_4)超声心动图提示致心律失常性右心室心肌病,余3例未发现器质性心脏病。1例患者为室早/室速伴有晕厥,另3例仅有室早。室早/室速体表心电图表现为下壁导联 R 波振幅高、心电轴右偏和 Q_(aVL)/Q_(aVR)比值较大。非接触式标测显示最早激动点位于球囊上方较远距离,激动面积大,最早激动点至爆发点距离远。成功靶点处激动较体表 QRS 波起始提前(28±6)ms,2例记录到等大的 A 波和 V 波,3例记录到融合的尖峰或碎裂电位,局部高能量可以获得较满意的起搏标测图形。4例患者均消融成功,随访(6.5±3.0)个月,1例复发后再次消融成功。结论起源于 MSPA 的室早/室速并非少见,非接触式标测的特殊表现可快速揭示诊断,详细的激动标测和起搏标测指导的消融具有较好的临床效果。
