Objective To investigate the apoptosis in gastric cancer induced by trichosanthin (TCS) and the relationship between this apoptosis and expression of c-myc. Methods In in vitro experiment, morphologic test and TUNEL s...Objective To investigate the apoptosis in gastric cancer induced by trichosanthin (TCS) and the relationship between this apoptosis and expression of c-myc. Methods In in vitro experiment, morphologic test and TUNEL staining method were used to detect quantitatively and qualitatively the apoptosis status of gastric adenocarcinoma cell line SGC-7901 before and after the treatment of 0.1μg/ml TCS. Immunohistochemical staining method and Northern Blot hybridization were used to detect the expression status of apoptosis-related genes c-myc before and after TCS treatment. Results Some typical apoptotic morphologic changes appeared after 36h treated by TCS. The apoptotic indexes increased significantly in the TCS treated group than those of the untreated group after 36h,42h and 48h (P<0.01). After 24h treated by TCS, an increased expression of c-myc protein product occurred, the staining density was increased from + or ++ to +++ (P< 0.01), and the expression of c-myc RNA increased significantly (P<0.05). Conclusion TCS is able to induce the apoptosis in gastric cancer. This apoptosis may be mediated by up-expression of apoptosis-regulated gene c-myc.展开更多
研究通过克隆亚麻纤维素合成酶Lusi Ces A1基因,利用抑制差减杂交(SSH)技术,以DIANE Lusi Ces A1基因敲除RNA为Tester,常规RNA为Driver,构建亚麻纤维素合成酶c DNA文库,逆向斑点杂交验证阳性克隆,筛选差异表达克隆,鉴定出Lusi Ces A1上...研究通过克隆亚麻纤维素合成酶Lusi Ces A1基因,利用抑制差减杂交(SSH)技术,以DIANE Lusi Ces A1基因敲除RNA为Tester,常规RNA为Driver,构建亚麻纤维素合成酶c DNA文库,逆向斑点杂交验证阳性克隆,筛选差异表达克隆,鉴定出Lusi Ces A1上调表达增强基因122个,其中95个与已知功能基因同源,其余27个未知同源序列推断为亚麻纤维素合成途径新基因。Lusi Ces A1是亚麻纤维素合成途径关键酶基因,通过其上调表达作用机理分析,挖掘纤维合成过程上调表达基因,可改良亚麻品种。展开更多
文摘Objective To investigate the apoptosis in gastric cancer induced by trichosanthin (TCS) and the relationship between this apoptosis and expression of c-myc. Methods In in vitro experiment, morphologic test and TUNEL staining method were used to detect quantitatively and qualitatively the apoptosis status of gastric adenocarcinoma cell line SGC-7901 before and after the treatment of 0.1μg/ml TCS. Immunohistochemical staining method and Northern Blot hybridization were used to detect the expression status of apoptosis-related genes c-myc before and after TCS treatment. Results Some typical apoptotic morphologic changes appeared after 36h treated by TCS. The apoptotic indexes increased significantly in the TCS treated group than those of the untreated group after 36h,42h and 48h (P<0.01). After 24h treated by TCS, an increased expression of c-myc protein product occurred, the staining density was increased from + or ++ to +++ (P< 0.01), and the expression of c-myc RNA increased significantly (P<0.05). Conclusion TCS is able to induce the apoptosis in gastric cancer. This apoptosis may be mediated by up-expression of apoptosis-regulated gene c-myc.
文摘研究通过克隆亚麻纤维素合成酶Lusi Ces A1基因,利用抑制差减杂交(SSH)技术,以DIANE Lusi Ces A1基因敲除RNA为Tester,常规RNA为Driver,构建亚麻纤维素合成酶c DNA文库,逆向斑点杂交验证阳性克隆,筛选差异表达克隆,鉴定出Lusi Ces A1上调表达增强基因122个,其中95个与已知功能基因同源,其余27个未知同源序列推断为亚麻纤维素合成途径新基因。Lusi Ces A1是亚麻纤维素合成途径关键酶基因,通过其上调表达作用机理分析,挖掘纤维合成过程上调表达基因,可改良亚麻品种。
