Eleven novel triterpenoid saponins,N-substituted-β-D-glucosaminide derivatives of oleanolic acid,were designed and synthesized via a stepwise glycosylation strategy.These compounds were evaluated for in vitro cytotox...Eleven novel triterpenoid saponins,N-substituted-β-D-glucosaminide derivatives of oleanolic acid,were designed and synthesized via a stepwise glycosylation strategy.These compounds were evaluated for in vitro cytotoxic activity against six different tumor cell lines.Most of the compounds inhibited the growth of,at least,one tumor cell line effectively at micromolar concentrations.Preliminary structure-activity relationships（SARs） indicate that acylation of the nitrogen of the glucosamine-bearing triterpenoid saponins affords the compounds that are highly cytotoxic towards specific tumor cell lines.展开更多
目的:应用rhIL-18在体外培养系统(Coculture system in vitro,CCs)中诱导肿瘤特异性细胞毒性T淋巴细胞(Cytotoxic TLymphocyte,CTL),探讨IFN-γ在rhIL-18诱导的肿瘤特异性CTL产生过程及杀伤效应中的作用。方法:采用StemSepTM免疫磁性细...目的:应用rhIL-18在体外培养系统(Coculture system in vitro,CCs)中诱导肿瘤特异性细胞毒性T淋巴细胞(Cytotoxic TLymphocyte,CTL),探讨IFN-γ在rhIL-18诱导的肿瘤特异性CTL产生过程及杀伤效应中的作用。方法:采用StemSepTM免疫磁性细胞分离法分离人外周血NK细胞、T细胞及DCs细胞,流式细胞仪分析细胞表型,125I-UdR标记的细胞毒实验检测杀伤活性,ELISA方法检测IFN-γ蛋白产生量,RT-PCR检测IFN-γmRNA表达含量。结果:在肿瘤抗原存在的条件下,IL-18在CCs中能够诱导并促进CTL介导的肿瘤特异性杀伤效应;IL-18能够在肿瘤抗原刺激的CCs中诱导IFN-γmRNA的表达及IFN-γ蛋白的产生,并与IL-18的含量呈剂量依赖关系,在rhIL-18含量为100ng时,培养上清中IFN-γ为4410±210pg/ml。但加入抗IFN-γ抗体对rhIL-18诱导的肿瘤特异性CTL产生过程无明显影响,不能抑制IL-18诱导的这种肿瘤特异性CTL的杀伤效应。结论:IL-18能够在肿瘤抗原刺激的CCs中有效地诱导CTL介导的肿瘤特异性杀伤效应,并诱导IFN-γ的产生,但其诱导肿瘤特异性CTL的产生过程及杀伤效应与IFN-γ无关。展开更多
Fusarium moniliforme is a predominant fungus found in fungus-contaminated food in Linxian County, China, a high risk area of esophageal. cancer. Rats fed with corn bread inoculated with Fusarium moniliforme developed ...Fusarium moniliforme is a predominant fungus found in fungus-contaminated food in Linxian County, China, a high risk area of esophageal. cancer. Rats fed with corn bread inoculated with Fusarium moniliforme developed papillomas and squamous-cell carcinomas展开更多
基金Supported by the National Natural Science Foundation of China（Nos.21142010, 81273358） and the PhD Scientific Research Opening Foundation Projects of Liaoning Province of China（No.20091081）.
文摘Eleven novel triterpenoid saponins,N-substituted-β-D-glucosaminide derivatives of oleanolic acid,were designed and synthesized via a stepwise glycosylation strategy.These compounds were evaluated for in vitro cytotoxic activity against six different tumor cell lines.Most of the compounds inhibited the growth of,at least,one tumor cell line effectively at micromolar concentrations.Preliminary structure-activity relationships（SARs） indicate that acylation of the nitrogen of the glucosamine-bearing triterpenoid saponins affords the compounds that are highly cytotoxic towards specific tumor cell lines.
文摘目的:应用rhIL-18在体外培养系统(Coculture system in vitro,CCs)中诱导肿瘤特异性细胞毒性T淋巴细胞(Cytotoxic TLymphocyte,CTL),探讨IFN-γ在rhIL-18诱导的肿瘤特异性CTL产生过程及杀伤效应中的作用。方法:采用StemSepTM免疫磁性细胞分离法分离人外周血NK细胞、T细胞及DCs细胞,流式细胞仪分析细胞表型,125I-UdR标记的细胞毒实验检测杀伤活性,ELISA方法检测IFN-γ蛋白产生量,RT-PCR检测IFN-γmRNA表达含量。结果:在肿瘤抗原存在的条件下,IL-18在CCs中能够诱导并促进CTL介导的肿瘤特异性杀伤效应;IL-18能够在肿瘤抗原刺激的CCs中诱导IFN-γmRNA的表达及IFN-γ蛋白的产生,并与IL-18的含量呈剂量依赖关系,在rhIL-18含量为100ng时,培养上清中IFN-γ为4410±210pg/ml。但加入抗IFN-γ抗体对rhIL-18诱导的肿瘤特异性CTL产生过程无明显影响,不能抑制IL-18诱导的这种肿瘤特异性CTL的杀伤效应。结论:IL-18能够在肿瘤抗原刺激的CCs中有效地诱导CTL介导的肿瘤特异性杀伤效应,并诱导IFN-γ的产生,但其诱导肿瘤特异性CTL的产生过程及杀伤效应与IFN-γ无关。
文摘Fusarium moniliforme is a predominant fungus found in fungus-contaminated food in Linxian County, China, a high risk area of esophageal. cancer. Rats fed with corn bread inoculated with Fusarium moniliforme developed papillomas and squamous-cell carcinomas
