目的探究雷公藤内酯醇(triptolide,TPL)对脂多糖(lipopolysaccharide,LPS)诱导的足细胞损伤模型的保护作用。方法以LPS诱导的足细胞损伤模型为研究对象,设立空白对照组、药物组、模型组和实验组。采用免疫荧光法观察足细胞肌动蛋白细胞...目的探究雷公藤内酯醇(triptolide,TPL)对脂多糖(lipopolysaccharide,LPS)诱导的足细胞损伤模型的保护作用。方法以LPS诱导的足细胞损伤模型为研究对象,设立空白对照组、药物组、模型组和实验组。采用免疫荧光法观察足细胞肌动蛋白细胞骨架的形态及粘着斑蛋白Vinculin的表达,激光共聚焦显微镜采集图像;Western blot检测各组血管生成素样蛋白3(angiopoietin-like protein 3,Angptl3)、CD2-相关蛋白(CD2-associated protein,CD2AP)、Synaptopodin以及Palladin的表达。结果TPL可以使足细胞细胞骨架排列趋于整齐,部分丝状结构完整,提高足细胞CD2AP(P<0.05)和Synaptopodin(P<0.001)、Palladin(P<0.05)及Vinculin的表达,同时可降低足细胞Angptl3的表达(P<0.05)。结论TPL可在一定程度上保护足细胞骨架,缓解LPS造成的细胞损伤。展开更多
Triptolide（TPL/TL） is a natural drug with novel anticancer effects. Preclinical studies indicated that TPL inhibits cell proliferation, induces cell apoptosis, inhibits tumor metastasis and enhances the effect of ot...Triptolide（TPL/TL） is a natural drug with novel anticancer effects. Preclinical studies indicated that TPL inhibits cell proliferation, induces cell apoptosis, inhibits tumor metastasis and enhances the effect of other therapeutic methods in various cancer cell lines. Multiple molecules and signaling pathways, such as caspases, heat-shock proteins, NF-κB, and deoxyribonucleic acid（DNA） repair-associated factors, are associated with the anti-cancer effect. TPL also improves chemoradiosensitivity in cancer therapy. Phase I trials indicate the potential clinical value of TPL use. However, further trials with larger sample sizes are needed to confirm these results.展开更多
文摘目的探究雷公藤内酯醇(triptolide,TPL)对脂多糖(lipopolysaccharide,LPS)诱导的足细胞损伤模型的保护作用。方法以LPS诱导的足细胞损伤模型为研究对象,设立空白对照组、药物组、模型组和实验组。采用免疫荧光法观察足细胞肌动蛋白细胞骨架的形态及粘着斑蛋白Vinculin的表达,激光共聚焦显微镜采集图像;Western blot检测各组血管生成素样蛋白3(angiopoietin-like protein 3,Angptl3)、CD2-相关蛋白(CD2-associated protein,CD2AP)、Synaptopodin以及Palladin的表达。结果TPL可以使足细胞细胞骨架排列趋于整齐,部分丝状结构完整,提高足细胞CD2AP(P<0.05)和Synaptopodin(P<0.001)、Palladin(P<0.05)及Vinculin的表达,同时可降低足细胞Angptl3的表达(P<0.05)。结论TPL可在一定程度上保护足细胞骨架,缓解LPS造成的细胞损伤。
文摘目的:为电针联合雷公藤甲素(triptolide,TPL)治疗类风湿关节炎(rheumatoid arthritis,RA),同时减轻TPL所致的肝损提供增效减毒的实验依据。方法:RA模型采用弗氏完全佐剂(freund’s complete adjuvant,CFA)建立,运用液相色谱-质谱联用技术(liquid chromatography-mass spectrometry,LC-MS)检测大鼠血浆TPL浓度,试剂盒测定肝匀浆中谷氨酸-丙酮酸转氨酶(ALT)、天冬氨酸氨基转移酶(AST)含量及超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)活性。结果:针药组大鼠足趾肿胀改善情况最好,药物组次之,再者为电针组;在同一时刻电针刺"足三里"后的血药浓度高于单独口服TPL的浓度,TPL的药动学参数:达峰时间(T_(max))提前,峰浓度(C_(max))及药时曲线下面积(area under the curve,AUC)提高;针药组ALT、AST含量及MDA活性均较药物组降低,SOD活性则升高。结论:电针"足三里"可以减轻RA大鼠足趾肿胀,提高TPL的血药浓度,进而提高其生物利用度,同时还可减轻TPL对RA大鼠肝组织的损伤,提高其抗氧化应激能力。
基金supported by a project funded by the Priority Academic Program Development of Jiangsu HigherEducation Institutions (PAPD) (JX10231801)grants from Key Academic Discipline of Jiangsu Province "Medical Aspects of Specific Environments"
文摘Triptolide（TPL/TL） is a natural drug with novel anticancer effects. Preclinical studies indicated that TPL inhibits cell proliferation, induces cell apoptosis, inhibits tumor metastasis and enhances the effect of other therapeutic methods in various cancer cell lines. Multiple molecules and signaling pathways, such as caspases, heat-shock proteins, NF-κB, and deoxyribonucleic acid（DNA） repair-associated factors, are associated with the anti-cancer effect. TPL also improves chemoradiosensitivity in cancer therapy. Phase I trials indicate the potential clinical value of TPL use. However, further trials with larger sample sizes are needed to confirm these results.
