摘要
目的探究雷公藤内酯醇(triptolide,TPL)对脂多糖(lipopolysaccharide,LPS)诱导的足细胞损伤模型的保护作用。方法以LPS诱导的足细胞损伤模型为研究对象,设立空白对照组、药物组、模型组和实验组。采用免疫荧光法观察足细胞肌动蛋白细胞骨架的形态及粘着斑蛋白Vinculin的表达,激光共聚焦显微镜采集图像;Western blot检测各组血管生成素样蛋白3(angiopoietin-like protein 3,Angptl3)、CD2-相关蛋白(CD2-associated protein,CD2AP)、Synaptopodin以及Palladin的表达。结果TPL可以使足细胞细胞骨架排列趋于整齐,部分丝状结构完整,提高足细胞CD2AP(P<0.05)和Synaptopodin(P<0.001)、Palladin(P<0.05)及Vinculin的表达,同时可降低足细胞Angptl3的表达(P<0.05)。结论TPL可在一定程度上保护足细胞骨架,缓解LPS造成的细胞损伤。
Objective To explore the protective effect of triptolide(TPL)on lipopolysaccharide(LPS)-induced podocyte injury.Methods An LPS-induced podocyte injury model in vitro was adopted in the experiment.The podocytes were divided into 4 groups:blank control group,drug group,model group,and experimental group.We observed the rearrangement of the podocyte cytoskeleton and detected the expression of focal adhesion protein Vinculin by immunofluorescence.The expression of CD2-associated protein(CD2AP),synaptopodin,palladin,angiopoietin-like protein 3(Angptl3)were detected by Western Blot.Results In vitro LPS-induced podocyte injury,TPL intervention resulted in a relatively neat podocyte cytoskeletal arrangement,a partial intact filamentous structure,and increased expressions of CD2AP(P<0.05),Synaptopodin(P<0.001),Palladin(P<0.05)and Vinculin.Meanwhile,it significantly reversed LPS-induced upregulation of Angptl3(P<0.05).Conclusion TPL is effective for protecting the podocyte cytoskeleton to some extent and alleviating the cell damage caused by LPS stimulation.
作者
齐芳艳
刘俊朝
俞建
徐虹
韩新利
汪永红
孙雯
QI Fang-yan;LIU Jun-chao;YU Jian;XU Hong;HAN Xin-li;WANG Yong-hong;SUN Wen(Department of Traditional Chinese Medicine,Children’s Hospital,Fudan University,Shanghai 201102,China;Department of Nephrology,Children’s Hospital,Fudan University,Shanghai 201102,China)
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2020年第5期679-685,共7页
Fudan University Journal of Medical Sciences
基金
国家自然科学基金(8157150258)。
