The authenticity of architectural heritage is interpreted differently in diverse cultural traditions.Can this inspire the conservation and contemporary regeneration of architectural heritage to adapt to the changing n...The authenticity of architectural heritage is interpreted differently in diverse cultural traditions.Can this inspire the conservation and contemporary regeneration of architectural heritage to adapt to the changing needs of modern life and society,rather than static exhibits in museums?This research focuses on Ganlan(pile-built timber construction)architecture to demonstrate how adaptive conservation and regeneration can keep traditional construction systems living and sustainable.Ganlan,a vernacular architectural archetype in the humid subtropical regions of Asia,has the following common features:free plans with an assembling frame structure,open and interactive envelopes to cope with the hot and damp climate,and stilts to deal with the rugged terrain.This research employed a threefold method of field investigation,topological deformation,and experimental design in examining Lianghekou,a historic Tujia village in Western Hubei,China.The findings reveal that Tujia people build their stilt houses with adaptable features to meet the changing functional requirements and variable topographies.These traditions can be transmitted into the contemporary design,as our experimental design illustrates.The self-adaptability and topological deformation of Ganlan architectural heritage demonstrate a unique perspective of understanding authenticity,and contribute toward innovative application in the conservation and regeneration of vernacular architecture.展开更多
The architectural parameters of Reaumuria soongorica root system in different habitats of Gansu Province, China were analyzed to examine its ecological adaptability to arid environments. Results show that： （1） Topo...The architectural parameters of Reaumuria soongorica root system in different habitats of Gansu Province, China were analyzed to examine its ecological adaptability to arid environments. Results show that： （1） Topological indices of R. Soongorica root sys- tem are small in all habitats, and root branching pattem tends to be dichotomous. Also, the indices gradually increase in the Min- qin windblown sand region and the Zhangye Gobi region in Hexi Corridor, which indicates that drought tends to produce her- ringbone-like root branching pattems. （2） Fractal dimension values ofR. Soongorica root system are small and not obvious in the Minqin windblown sand region and the Zhangye Gobi region in Hexi Corridor, with values of 1.1778 and 1.1169, respectively. Fractal dimension values are relatively large in Jiuzhoutai semi-arid hilly and gully region of the Loess Plateau, which indicates that the R. Soongorica root system has better fractal characteristics in this region than in the other regions. （3） Total branching ra- tios of the R. Soongorica root system in arid regions of Hexi Corridor are smaller than that in the Jiuzhoutai semi-arid hilly and gully region of the Loess Plateau. This shows that root branching ability in the semi-arid region is stronger, and it decreases to some degree with increased drought. （4） The root connection lengths of R. soongorica root system are long in all habitats, but there are significant length differences between the different habitats. The root connection length at the Minqin windblown sand region is the longest. It is concluded that R. soongoriea adapts to arid environments by decreasing root branching, decreasing root overlap and increasing root connection length, which makes its root branching pattern tend to be herringbone-like to reduce com- petition in root internal environment for nutrients and to enhance root absorption rate of nutrients, and ensure effective nutrition space. Thus the roots can absorb enough water and nutrients in resource-poor settings to 展开更多
The genomes are organized into ordered and hierarchical topological structures in interphase nuclei.Within discrete territories of each chromosome,topologically associated domains（TADs） play important roles in vario...The genomes are organized into ordered and hierarchical topological structures in interphase nuclei.Within discrete territories of each chromosome,topologically associated domains（TADs） play important roles in various nuclear processes such as gene regulation.Inside TADs separated by relatively constitutive boundaries,distal elements regulate their gene targets through specific chromatin-looping contacts such as long-distance enhancer-promoter interactions.High-throughput sequencing studies have revealed millions of potential regulatory DNA elements,which are much more abundant than the mere ~ 20,000 genes they control.The recently emerged CRISPRCas9 genome editing technologies have enabled efficient and precise genetic and epigenetic manipulations of genomes.The multiplexed and high-throughput CRISPR capabilities facilitate the discovery and dissection of gene regulatory elements.Here,we describe the applications of CRISPR for genome,epigenome,and 3D genome editing,focusing on CRISPR DNA-fragment editing with Cas9 and a pair of sgRNAs to investigate topological folding of chromatin TADs and developmental gene regulation.展开更多
基金the Chinese National Social Science Funding Project"Visual Image Cluster Analysis and Atlas Composing of the Chinese Nation"(20VMZ008)"Research and Practice Fund for New Engineering Education of the Ministry of Education of China(2nd Batch)"(ETMUZSLHY20202123).
文摘The authenticity of architectural heritage is interpreted differently in diverse cultural traditions.Can this inspire the conservation and contemporary regeneration of architectural heritage to adapt to the changing needs of modern life and society,rather than static exhibits in museums?This research focuses on Ganlan(pile-built timber construction)architecture to demonstrate how adaptive conservation and regeneration can keep traditional construction systems living and sustainable.Ganlan,a vernacular architectural archetype in the humid subtropical regions of Asia,has the following common features:free plans with an assembling frame structure,open and interactive envelopes to cope with the hot and damp climate,and stilts to deal with the rugged terrain.This research employed a threefold method of field investigation,topological deformation,and experimental design in examining Lianghekou,a historic Tujia village in Western Hubei,China.The findings reveal that Tujia people build their stilt houses with adaptable features to meet the changing functional requirements and variable topographies.These traditions can be transmitted into the contemporary design,as our experimental design illustrates.The self-adaptability and topological deformation of Ganlan architectural heritage demonstrate a unique perspective of understanding authenticity,and contribute toward innovative application in the conservation and regeneration of vernacular architecture.
基金funded by the National Natural Science Foundation of China(4136110031360205)+1 种基金International Science and Technology Cooperation Program of China(2012DFR30830)the Gansu Science and Technology Support Program(1204NKCA084)
文摘The architectural parameters of Reaumuria soongorica root system in different habitats of Gansu Province, China were analyzed to examine its ecological adaptability to arid environments. Results show that： （1） Topological indices of R. Soongorica root sys- tem are small in all habitats, and root branching pattem tends to be dichotomous. Also, the indices gradually increase in the Min- qin windblown sand region and the Zhangye Gobi region in Hexi Corridor, which indicates that drought tends to produce her- ringbone-like root branching pattems. （2） Fractal dimension values ofR. Soongorica root system are small and not obvious in the Minqin windblown sand region and the Zhangye Gobi region in Hexi Corridor, with values of 1.1778 and 1.1169, respectively. Fractal dimension values are relatively large in Jiuzhoutai semi-arid hilly and gully region of the Loess Plateau, which indicates that the R. Soongorica root system has better fractal characteristics in this region than in the other regions. （3） Total branching ra- tios of the R. Soongorica root system in arid regions of Hexi Corridor are smaller than that in the Jiuzhoutai semi-arid hilly and gully region of the Loess Plateau. This shows that root branching ability in the semi-arid region is stronger, and it decreases to some degree with increased drought. （4） The root connection lengths of R. soongorica root system are long in all habitats, but there are significant length differences between the different habitats. The root connection length at the Minqin windblown sand region is the longest. It is concluded that R. soongoriea adapts to arid environments by decreasing root branching, decreasing root overlap and increasing root connection length, which makes its root branching pattern tend to be herringbone-like to reduce com- petition in root internal environment for nutrients and to enhance root absorption rate of nutrients, and ensure effective nutrition space. Thus the roots can absorb enough water and nutrients in resource-poor settings to
基金supported by the National Natural Science Foundation for the Youth of China(No.81302861)the National Natural Science Foundation of China(No.91519302)the Science and Technology Commission of Shanghai Municipality(No.14JC1403600)
文摘The genomes are organized into ordered and hierarchical topological structures in interphase nuclei.Within discrete territories of each chromosome,topologically associated domains（TADs） play important roles in various nuclear processes such as gene regulation.Inside TADs separated by relatively constitutive boundaries,distal elements regulate their gene targets through specific chromatin-looping contacts such as long-distance enhancer-promoter interactions.High-throughput sequencing studies have revealed millions of potential regulatory DNA elements,which are much more abundant than the mere ~ 20,000 genes they control.The recently emerged CRISPRCas9 genome editing technologies have enabled efficient and precise genetic and epigenetic manipulations of genomes.The multiplexed and high-throughput CRISPR capabilities facilitate the discovery and dissection of gene regulatory elements.Here,we describe the applications of CRISPR for genome,epigenome,and 3D genome editing,focusing on CRISPR DNA-fragment editing with Cas9 and a pair of sgRNAs to investigate topological folding of chromatin TADs and developmental gene regulation.
