OBJECTIVE: To investigate the role of apoptosis in radiation-induced mouse thymus lymphocyte damage and repair and provide the basis for understanding the molecular mechanism of radiation-induced lymphocyte damage and...OBJECTIVE: To investigate the role of apoptosis in radiation-induced mouse thymus lymphocyte damage and repair and provide the basis for understanding the molecular mechanism of radiation-induced lymphocyte damage and repair as well as the prevention and treatment of acute radiation sickness. METHODS: We studied the dynamic changes of apoptosis of mouse thymus lymphocytes and the expression of bax and bcl-2 gene products after 2, 4, 6 and 8 Gy of whole body gamma-irradiation using in situ terminal labeling, DNA electrophoresis and immunohistochemical techniques. RESULTS: At the early stage after irradiation, the percentage of apoptotic lymphocytes increased rapidly in accordance with the increasing of radiation doses, while the counts of the thymus and peripheral lymphocytes decreased sharply, showing an opposite change to lymphocyte apoptosis. After 6 Gy gamma-irradiation, typical morphological characteristics of thymus apoptotic lymphocytes in early, middle and late stages were found by transmission electron microscopy. The thymus lymphocytes displayed characteristic DNA ladders 4 hr and 8 hr after 2-6 Gy gamma-irradiation,using DNA gel electrophoresis techniques. Abnormal expression of bcl-2 and bax gene products were shown in irradiated lymphocytes. CONCLUSIONS: Apoptosis plays an important role in the process of radiation-induced mouse thymus lymphocyte damage and repair. Bcl-2 and Bax proteins may regulate the process of lymphocyte apoptosis.展开更多
目的探究对重症肌无力合并胸腺病变的患者血清中乙酰胆碱受体(acetylcholine receptors,AchR)抗体、肌联蛋白(Titin)抗体和肌肉特异性酪氨酸激酶(muscle specific tyrosine kinase,MuSK)抗体的检测意义。方法选取我院收治的80例重症肌...目的探究对重症肌无力合并胸腺病变的患者血清中乙酰胆碱受体(acetylcholine receptors,AchR)抗体、肌联蛋白(Titin)抗体和肌肉特异性酪氨酸激酶(muscle specific tyrosine kinase,MuSK)抗体的检测意义。方法选取我院收治的80例重症肌无力患者,根据是否存在胸腺病变分为胸腺病变组48例和非胸腺病变组32例,另取80例健康个体作为对照组,通过免疫酶联吸附试验对3组成员血清中AchR抗体、Titin抗体和MuSK抗体进行检测并比较。结果重症肌无力患者血清AchR抗体和Titin抗体阳性率(76.3%,52.5%)均明显高于对照组(11.3%,5.0%)(P<0.05);重症肌无力组及对照组患者血清MuSK抗体阳性率均为0,但前者血清中MuSK抗体水平明显高于后者(P<0.05);AchR,Titin两种抗体联合检测可明显提高检测重症肌无力的灵敏度;AchR和Titin抗体在胸腺病变组中的阳性率明显高于非胸腺病变组(P<0.05);全身型重症肌无力患者的血清AchR抗体和Titin抗体阳性率明显高于眼肌型(P<0.05)。结论 AchR抗体和Titin抗体的阳性率与重症肌无力患者的病情相关,病情严重或合并胸腺病变可明显提高两者的阳性率,此外两者联合检测可提高诊断的灵敏度。而MuSK抗体在我国重症肌无力患者中的阳性率较低。展开更多
为研究由炒白术、山楂、黄芪、板蓝根、茯苓、陈皮、甘草、山药制成的复方中药制剂对SD大鼠胸腺组织结构及其Caspase-3蛋白分布的影响。选择60只SPF级断奶SD大鼠,随机分为对照组、1.5 m L·kg^(-1)组、3.0 m L·kg^(-1)组和6.0 ...为研究由炒白术、山楂、黄芪、板蓝根、茯苓、陈皮、甘草、山药制成的复方中药制剂对SD大鼠胸腺组织结构及其Caspase-3蛋白分布的影响。选择60只SPF级断奶SD大鼠,随机分为对照组、1.5 m L·kg^(-1)组、3.0 m L·kg^(-1)组和6.0 m L·kg^(-1)组,每组15只,预饲1周后按每千克体质量分别定时灌胃3.0 m L蒸馏水、1.5m L、3.0 m L和6.0 m L药液(每m L含1 g生药)。至90 d,各处理组SD大鼠禁食禁水3 h,称量、麻醉、处死后取完整的胸腺称量,立即投入4%的多聚甲醛磷酸缓冲液固定72 h,制作石蜡组织切片,经HE、网状纤维和Caspase-3免疫组化染色,显微观察,Image-pro PLUS 6.0软件分析并摄影。结果表明,按每kg体质量灌胃本复方中药制剂3.0 m L可显著增加胸腺皮质区且胸腺的生长指数显著高于对照组(P<0.05)。网状纤维主要分布于SD大鼠胸腺髓质部,3.0 m L·kg^(-1)剂量组网状纤维含量较对照组显著减少。各实验组均不同程度抑制Caspase-3蛋白的出现,其中以3.0 m L·kg^(-1)剂量组最为显著。说明灌胃3.0 m L·kg^(-1)复方中药制剂可改善SD大鼠胸腺组织结构,延缓其结构和功能退化。展开更多
文摘OBJECTIVE: To investigate the role of apoptosis in radiation-induced mouse thymus lymphocyte damage and repair and provide the basis for understanding the molecular mechanism of radiation-induced lymphocyte damage and repair as well as the prevention and treatment of acute radiation sickness. METHODS: We studied the dynamic changes of apoptosis of mouse thymus lymphocytes and the expression of bax and bcl-2 gene products after 2, 4, 6 and 8 Gy of whole body gamma-irradiation using in situ terminal labeling, DNA electrophoresis and immunohistochemical techniques. RESULTS: At the early stage after irradiation, the percentage of apoptotic lymphocytes increased rapidly in accordance with the increasing of radiation doses, while the counts of the thymus and peripheral lymphocytes decreased sharply, showing an opposite change to lymphocyte apoptosis. After 6 Gy gamma-irradiation, typical morphological characteristics of thymus apoptotic lymphocytes in early, middle and late stages were found by transmission electron microscopy. The thymus lymphocytes displayed characteristic DNA ladders 4 hr and 8 hr after 2-6 Gy gamma-irradiation,using DNA gel electrophoresis techniques. Abnormal expression of bcl-2 and bax gene products were shown in irradiated lymphocytes. CONCLUSIONS: Apoptosis plays an important role in the process of radiation-induced mouse thymus lymphocyte damage and repair. Bcl-2 and Bax proteins may regulate the process of lymphocyte apoptosis.
文摘为研究由炒白术、山楂、黄芪、板蓝根、茯苓、陈皮、甘草、山药制成的复方中药制剂对SD大鼠胸腺组织结构及其Caspase-3蛋白分布的影响。选择60只SPF级断奶SD大鼠,随机分为对照组、1.5 m L·kg^(-1)组、3.0 m L·kg^(-1)组和6.0 m L·kg^(-1)组,每组15只,预饲1周后按每千克体质量分别定时灌胃3.0 m L蒸馏水、1.5m L、3.0 m L和6.0 m L药液(每m L含1 g生药)。至90 d,各处理组SD大鼠禁食禁水3 h,称量、麻醉、处死后取完整的胸腺称量,立即投入4%的多聚甲醛磷酸缓冲液固定72 h,制作石蜡组织切片,经HE、网状纤维和Caspase-3免疫组化染色,显微观察,Image-pro PLUS 6.0软件分析并摄影。结果表明,按每kg体质量灌胃本复方中药制剂3.0 m L可显著增加胸腺皮质区且胸腺的生长指数显著高于对照组(P<0.05)。网状纤维主要分布于SD大鼠胸腺髓质部,3.0 m L·kg^(-1)剂量组网状纤维含量较对照组显著减少。各实验组均不同程度抑制Caspase-3蛋白的出现,其中以3.0 m L·kg^(-1)剂量组最为显著。说明灌胃3.0 m L·kg^(-1)复方中药制剂可改善SD大鼠胸腺组织结构,延缓其结构和功能退化。
