[目的]建立高效液相色谱法测定大豆及3种豆制品中大豆异黄酮组分和含量的方法,研究其大豆异黄酮组分的差异。[方法]将供试样品用80%甲醇溶解提取,净化后用Wonda Sil C_(18)WR(4.6×250 mm,5μm)色谱柱分离,以乙腈和磷酸水溶液(p H 3...[目的]建立高效液相色谱法测定大豆及3种豆制品中大豆异黄酮组分和含量的方法,研究其大豆异黄酮组分的差异。[方法]将供试样品用80%甲醇溶解提取,净化后用Wonda Sil C_(18)WR(4.6×250 mm,5μm)色谱柱分离,以乙腈和磷酸水溶液(p H 3.0)作为流动相,流速1 m L/min,柱温为40℃,用二极管阵列检测器在波长260 nm检测大豆异黄酮组分,用外标法进行定量。[结果]大豆及3种豆制品中大豆异黄酮的含量,大豆最高,其次是豆芽、豆腐、豆浆。供试样品的大豆异黄酮组分中大豆苷相关系数为0.997 2,黄豆黄苷相关系数为0.999 6,大豆苷元相关系数为0.994 7,黄豆黄素相关系数为0.999 1,染料木素相关系数为0.994 2,平行测定结果之差均低于算术平均值的10%。[结论]高效液相色谱法可用于豆制品中大豆异黄酮组分和含量检测。展开更多
Taking soybean products as an example and using the daily price data of 2007-2015,this paper established the error correction model and BEKK-GARCH model,and made an empirical study on the spillover effect of futures a...Taking soybean products as an example and using the daily price data of 2007-2015,this paper established the error correction model and BEKK-GARCH model,and made an empirical study on the spillover effect of futures and spot price of agricultural products of China. According to this study,there were mean spillover effect and two-way volatility spillover effect in futures and spot price of soybean,soybean oil,and soybean meal; soybean futures prices significantly guided the spot price; in the price linkage between the types,the price relationship between the soybean meal and soybean was closer than between the soybean oil and soybean.展开更多
分别从DNA和蛋白质2个水平上对转基因耐除草剂大豆及其产品的检测方法进行了研究.设计合成引物检测大豆内参照基因Lectin(大豆凝集素)及转基因抗除草剂Roundup Ready大豆外源基因,包括来自土壤细菌Agrobacterium tum efaciens株系CP4的5...分别从DNA和蛋白质2个水平上对转基因耐除草剂大豆及其产品的检测方法进行了研究.设计合成引物检测大豆内参照基因Lectin(大豆凝集素)及转基因抗除草剂Roundup Ready大豆外源基因,包括来自土壤细菌Agrobacterium tum efaciens株系CP4的5-烯醇丙酮酸莽草酸-3-磷酸合酶(5-enolpyruvylsh ik im ate-3-phosphate synthase,EPSPS)基因、花椰菜花叶病毒(Cau liflowermosaic virus,CaMV)35S启动子、胭脂碱合酶3′端的转录终止子(nopalinesynthase,NOS).应用优化的常规PCR方法,对大豆及其加工产品进行了检测,检测灵敏度可达0.1%.通过PCR方法从转基因大豆中扩增出CP4-EPSPS基因,在大肠杆菌中表达,利用表达的外源蛋白质作为抗原免疫家兔,得到该蛋白质的多克隆抗体,并建立了一套基于蛋白质印迹杂交(western hybrid ization)的检测转基因大豆及其粗加工品的方法,其检测极限达到1%以下.此两方法互相配合,互相印证,有助于规范化转基因检测方法的建立.展开更多
文摘[目的]建立高效液相色谱法测定大豆及3种豆制品中大豆异黄酮组分和含量的方法,研究其大豆异黄酮组分的差异。[方法]将供试样品用80%甲醇溶解提取,净化后用Wonda Sil C_(18)WR(4.6×250 mm,5μm)色谱柱分离,以乙腈和磷酸水溶液(p H 3.0)作为流动相,流速1 m L/min,柱温为40℃,用二极管阵列检测器在波长260 nm检测大豆异黄酮组分,用外标法进行定量。[结果]大豆及3种豆制品中大豆异黄酮的含量,大豆最高,其次是豆芽、豆腐、豆浆。供试样品的大豆异黄酮组分中大豆苷相关系数为0.997 2,黄豆黄苷相关系数为0.999 6,大豆苷元相关系数为0.994 7,黄豆黄素相关系数为0.999 1,染料木素相关系数为0.994 2,平行测定结果之差均低于算术平均值的10%。[结论]高效液相色谱法可用于豆制品中大豆异黄酮组分和含量检测。
基金Supported by the Project of National Natural Science Foundation of China"Study on Risk Evaluation and Transmission of Agricultural Product Futures and Spot Market in China in the Context of Finance"(71673103)
文摘Taking soybean products as an example and using the daily price data of 2007-2015,this paper established the error correction model and BEKK-GARCH model,and made an empirical study on the spillover effect of futures and spot price of agricultural products of China. According to this study,there were mean spillover effect and two-way volatility spillover effect in futures and spot price of soybean,soybean oil,and soybean meal; soybean futures prices significantly guided the spot price; in the price linkage between the types,the price relationship between the soybean meal and soybean was closer than between the soybean oil and soybean.
文摘分别从DNA和蛋白质2个水平上对转基因耐除草剂大豆及其产品的检测方法进行了研究.设计合成引物检测大豆内参照基因Lectin(大豆凝集素)及转基因抗除草剂Roundup Ready大豆外源基因,包括来自土壤细菌Agrobacterium tum efaciens株系CP4的5-烯醇丙酮酸莽草酸-3-磷酸合酶(5-enolpyruvylsh ik im ate-3-phosphate synthase,EPSPS)基因、花椰菜花叶病毒(Cau liflowermosaic virus,CaMV)35S启动子、胭脂碱合酶3′端的转录终止子(nopalinesynthase,NOS).应用优化的常规PCR方法,对大豆及其加工产品进行了检测,检测灵敏度可达0.1%.通过PCR方法从转基因大豆中扩增出CP4-EPSPS基因,在大肠杆菌中表达,利用表达的外源蛋白质作为抗原免疫家兔,得到该蛋白质的多克隆抗体,并建立了一套基于蛋白质印迹杂交(western hybrid ization)的检测转基因大豆及其粗加工品的方法,其检测极限达到1%以下.此两方法互相配合,互相印证,有助于规范化转基因检测方法的建立.
