摘要
[目的]建立高效液相色谱法测定大豆及3种豆制品中大豆异黄酮组分和含量的方法,研究其大豆异黄酮组分的差异。[方法]将供试样品用80%甲醇溶解提取,净化后用Wonda Sil C_(18)WR(4.6×250 mm,5μm)色谱柱分离,以乙腈和磷酸水溶液(p H 3.0)作为流动相,流速1 m L/min,柱温为40℃,用二极管阵列检测器在波长260 nm检测大豆异黄酮组分,用外标法进行定量。[结果]大豆及3种豆制品中大豆异黄酮的含量,大豆最高,其次是豆芽、豆腐、豆浆。供试样品的大豆异黄酮组分中大豆苷相关系数为0.997 2,黄豆黄苷相关系数为0.999 6,大豆苷元相关系数为0.994 7,黄豆黄素相关系数为0.999 1,染料木素相关系数为0.994 2,平行测定结果之差均低于算术平均值的10%。[结论]高效液相色谱法可用于豆制品中大豆异黄酮组分和含量检测。
[Objective] A HPLC method for determining the components and content of soybean isoflavone in four soybean products was established.[Method] Samples were extracted with 80% methanol. The HPLC separation was performed on a Wonda Sil C_（18）WR（ 4. 6 × 250 mm,5 μm） by isocratic elution with acetonitrile and phosphoric acid water solution（ p H 3. 0） as mobile phase,flow rate 1 m L/min,column temperature 40 ℃,and 260 nm was detected by diode array detector.[Result]The order of soybean isoflavone content was soybean bean sprouts tofu and soy milk. The correlation coefficients of the Daidzin,Glycitin,Daidzein,Glycitin,Genistein were 0. 997 2,0. 999 6,0. 994 7,0. 999 1,0. 994 2,respectively. The difference of the parallel test results was lower than that of the arithmetic mean as 10%. [Conclusion] The HPLC method can be used for the detection of soybean isoflavone components and contents in soybean products.
出处
《安徽农业科学》
CAS
2016年第9期119-121,共3页
Journal of Anhui Agricultural Sciences
