Objective To isolate and characterize indigenous algicidal bacteria and their algae-lysing compounds active against Microcystis aeruginosa, strains TH1, TH2, and FACHB 905. Methods The bacteria were identified using t...Objective To isolate and characterize indigenous algicidal bacteria and their algae-lysing compounds active against Microcystis aeruginosa, strains TH1, TH2, and FACHB 905. Methods The bacteria were identified using the Biolog automated microbial identification system and 16S rDNA sequence analysis. The algae-lysing compounds were isolated and purified by silica gel column chromatography and reverse-phase high performance liquid chromatography. Their structures were confirmed by Nuclear Magnetic Resonance （NMR） and Fourier Transform infrared （FT-IR） spectroscopy. Algae-lysing activity was observed using microscopy. Results The algae-lysing bacterium LTH-2 isolated from Lake Taihu was identified as Serratia marcescens. Strain LTH-2 secreted a red pigment identified as prodigiosin （C20H25N30）, which showed strong lytic activity with algal strains M. aeruginoso TH1, TH2, and FACHB 905 in a concentration-dependent manner. The 50% inhibitory concentration （ICs0） of prodigiosin with the algal strains was 4.8 （±0.4）×10^-2 μg/mL, 8.9 （±1.1）×10^-2μg/mL, and 1.7 （±0.1）×10^-1 μg/mL in 24 h, respectively. Conclusion The bacterium LTH-2 and its pigment related to damage of cell membranes. The bacterium for regulating blooms of harmful M. aeruginosa. had strong Microcystis-lysing activity probably LTH-2 and its red pigment are potentially useful展开更多
This paper reports on the degradation of phenols and anilines using hydrogen peroxide as oxidizer and enzyme from Serratia marcescens Y-SM01 as catalyst. First, the COD Cr values before and after degradation were meas...This paper reports on the degradation of phenols and anilines using hydrogen peroxide as oxidizer and enzyme from Serratia marcescens Y-SM01 as catalyst. First, the COD Cr values before and after degradation were measured. Then, changes of molecular structures during the process were investigated with UV-VIS, IR and HPLC. It was found that after degradation, for some compounds such as hydroquinone, peaks on UV-VIS and IR spectra which belong to phenyl ring all disappeared, and HPLC found small molecules such as organic compounds. So the mechanism was clarified that in the degradation the phenyl ring was cleaved and small molecules were introduced, which just caused the COD Cr values of degradable compounds such as hydroquinone to decline greatly. Also, the connection between degradation properties and structures of phenols and anilines was analyzed, and the conclusion was as follows: di-/tri-phenols/anilines were easier to degrade than mono-phenols/anilines; ortho-/para-substituted compounds were easier to degrade than meta-substituted compounds; electron-attracting substituents such as —OH , —NH2 , —OCH 3 made degradation easier, while electron-withdrawing substituents such as —NO2 , —Cl , —COOH made degradation more difficult.展开更多
为获得能高效合成灵菌红素的菌株,首先对土壤中的微生物进行分离,筛选得到一株优势菌株.分别采用形态学、生理生化实验以及16SrDNA测序分析,鉴定该菌株为粘质沙雷氏菌属,命名为Serratiamarcescens ZX-01.对该菌株分别采用常压室温等离子...为获得能高效合成灵菌红素的菌株,首先对土壤中的微生物进行分离,筛选得到一株优势菌株.分别采用形态学、生理生化实验以及16SrDNA测序分析,鉴定该菌株为粘质沙雷氏菌属,命名为Serratiamarcescens ZX-01.对该菌株分别采用常压室温等离子体(Atmospheric and Room Temperature Plasma,ARTP)诱变,并进行平板初筛和摇瓶复筛.经多轮诱变后,得到一株灵菌红素优势菌株ZX-3-10,其产量可达128.7mg/L,比诱变前提高123%.五代产量稳定性试验结果表明,该菌株具有良好的产量传代稳定性.展开更多
基金supported by the National Science and Technology Major Project (2012ZX07101-005)the National Natural Science Foundation of China (30972440)Jiangsu Province Postgraduate Innovation Project (CX10B-087Z)
文摘Objective To isolate and characterize indigenous algicidal bacteria and their algae-lysing compounds active against Microcystis aeruginosa, strains TH1, TH2, and FACHB 905. Methods The bacteria were identified using the Biolog automated microbial identification system and 16S rDNA sequence analysis. The algae-lysing compounds were isolated and purified by silica gel column chromatography and reverse-phase high performance liquid chromatography. Their structures were confirmed by Nuclear Magnetic Resonance （NMR） and Fourier Transform infrared （FT-IR） spectroscopy. Algae-lysing activity was observed using microscopy. Results The algae-lysing bacterium LTH-2 isolated from Lake Taihu was identified as Serratia marcescens. Strain LTH-2 secreted a red pigment identified as prodigiosin （C20H25N30）, which showed strong lytic activity with algal strains M. aeruginoso TH1, TH2, and FACHB 905 in a concentration-dependent manner. The 50% inhibitory concentration （ICs0） of prodigiosin with the algal strains was 4.8 （±0.4）×10^-2 μg/mL, 8.9 （±1.1）×10^-2μg/mL, and 1.7 （±0.1）×10^-1 μg/mL in 24 h, respectively. Conclusion The bacterium LTH-2 and its pigment related to damage of cell membranes. The bacterium for regulating blooms of harmful M. aeruginosa. had strong Microcystis-lysing activity probably LTH-2 and its red pigment are potentially useful
文摘This paper reports on the degradation of phenols and anilines using hydrogen peroxide as oxidizer and enzyme from Serratia marcescens Y-SM01 as catalyst. First, the COD Cr values before and after degradation were measured. Then, changes of molecular structures during the process were investigated with UV-VIS, IR and HPLC. It was found that after degradation, for some compounds such as hydroquinone, peaks on UV-VIS and IR spectra which belong to phenyl ring all disappeared, and HPLC found small molecules such as organic compounds. So the mechanism was clarified that in the degradation the phenyl ring was cleaved and small molecules were introduced, which just caused the COD Cr values of degradable compounds such as hydroquinone to decline greatly. Also, the connection between degradation properties and structures of phenols and anilines was analyzed, and the conclusion was as follows: di-/tri-phenols/anilines were easier to degrade than mono-phenols/anilines; ortho-/para-substituted compounds were easier to degrade than meta-substituted compounds; electron-attracting substituents such as —OH , —NH2 , —OCH 3 made degradation easier, while electron-withdrawing substituents such as —NO2 , —Cl , —COOH made degradation more difficult.
文摘为获得能高效合成灵菌红素的菌株,首先对土壤中的微生物进行分离,筛选得到一株优势菌株.分别采用形态学、生理生化实验以及16SrDNA测序分析,鉴定该菌株为粘质沙雷氏菌属,命名为Serratiamarcescens ZX-01.对该菌株分别采用常压室温等离子体(Atmospheric and Room Temperature Plasma,ARTP)诱变,并进行平板初筛和摇瓶复筛.经多轮诱变后,得到一株灵菌红素优势菌株ZX-3-10,其产量可达128.7mg/L,比诱变前提高123%.五代产量稳定性试验结果表明,该菌株具有良好的产量传代稳定性.
