Although gonadotropin releasing hormone (GnRH), GnRH like molecule, and GnRH receptor (GnRH R) have been reported to exist in several tissues other than brain or anterior pituitary, there are no reports concerning ...Although gonadotropin releasing hormone (GnRH), GnRH like molecule, and GnRH receptor (GnRH R) have been reported to exist in several tissues other than brain or anterior pituitary, there are no reports concerning GnRH or GnRH R gene expression in a normal pancreatic gland. In order to define the production of GnRH as well as GnRH R in the pancreatic gland, we examined their gene expression in various developmental stages of rat pancreas using the reverse transcriptase polymerase chain reaction (RT PCR). GnRH mRNA transcripts were found in pancreas of male and female rats at different ages, expressing at about the same level, whereas GnRH R mRNA transcripts could not be detected in any rat pancreatic gland samples. These results suggest a possible biological role of GnRH in rodent pancreas. 展开更多
观察生长抑制和DNA损伤基因45(Growth arrest and DNA damage gene 45,GADD45)X射线照射后表达的改变,并研究其与照射剂量之间的关系。外周血给予0-5 GyX线照射后分离出单个核细胞并培养,在不同时间点上用反转录聚合酶链反应(Reverse tr...观察生长抑制和DNA损伤基因45(Growth arrest and DNA damage gene 45,GADD45)X射线照射后表达的改变,并研究其与照射剂量之间的关系。外周血给予0-5 GyX线照射后分离出单个核细胞并培养,在不同时间点上用反转录聚合酶链反应(Reverse transcriptase-Polymerase clain reaction,RT-PCR)的方法测定 GADD45基因的相对表达量,分析该基因的剂量、效应关系。照射后GADD45基因在转录水平表达呈剂量依赖性增加,照射后4h达峰值,以后开始下降,但在24h仍未恢复到初始水平。展开更多
基金the National Natural Sciences Foundationof China(No. 39770 388)
文摘Although gonadotropin releasing hormone (GnRH), GnRH like molecule, and GnRH receptor (GnRH R) have been reported to exist in several tissues other than brain or anterior pituitary, there are no reports concerning GnRH or GnRH R gene expression in a normal pancreatic gland. In order to define the production of GnRH as well as GnRH R in the pancreatic gland, we examined their gene expression in various developmental stages of rat pancreas using the reverse transcriptase polymerase chain reaction (RT PCR). GnRH mRNA transcripts were found in pancreas of male and female rats at different ages, expressing at about the same level, whereas GnRH R mRNA transcripts could not be detected in any rat pancreatic gland samples. These results suggest a possible biological role of GnRH in rodent pancreas.
