摘要
目的 探讨c myc癌基因表达与膀胱移行上皮细胞癌 (TCC)生物学行为的关系。方法 应用RT PCR法检测 2 7例TCC组织、16例癌旁组织和 16例正常膀胱粘膜组织c myc癌基因的mRNA表达。 结果 TCC组织中c myc表达阳性率为6 6 7% (18/ 2 7) ,癌旁组织的阳性率为 37 5 % (6 / 16 )而正常膀胱粘膜为 2 5 % (4/ 16 ) ,三者比较具有统计学差异 (P <0 0 5 ) ,不同临床分期和病理分级及肿瘤浸润、复发的TCC组织c myc表达阳性率无显著性差异 (P >0 0 5 )。结论 TCC组织c myc表达阳性率与癌旁组织和正常膀胱粘膜的阳性表达有差异 ,而TCC的不同分期、分级、浸润及复发等生物学行为与c myc表达未见差异。因此 ,单独将c myc作为TCC的诊断指标时 ,其特异性和灵敏度不够 ,需结合其他有关肿瘤标志物进行诊断和鉴别诊断。
Objective To investigate c myc oncogene expression in transitional cell carcinoma of the bladder(TCC) and its relation to the biological malignant potentials of TCC.Methods C myc was examined in 27 TCC tissues,16 adjacent tisses and 16 normal bladder mucosae by using reverse transcriptase polymerase chain reaction(RT PCR) assay.Results C myc was detected in 18 of 27(66 7%) TCC tissues,6 in 16(37 5%)adjacent tissues and 4 of 16 normal bladder mucosa(25 0%),the positive rate of c myc in TCC was significantly higher than that in adjacent and normal bladder tissues( P <0 05). There was no significant correlation between the positive rate of c myc and the clinicopathologic features of TCC, including clinical stage,pathologic grade,tumor invasion and tumor recurrence( P >0 05).Conclusion C myc may be used as a tumor marker in the diagnosis and differential diagnosis of TCC when it conbined with other tumor markers.
出处
《重庆医学》
CAS
CSCD
2002年第11期1043-1044,共2页
Chongqing medicine
关键词
膀胱肿瘤
C-MYC
逆转录聚合酶链反应
bladder neoplasms
c myc
reverse transcriptase polymerase chain reaction(RT PCR)
