AIM: To investigate the frequency of mutations in pre-core (pre-C) and basic core promoter (BCP) regions of hepatitis B virus (HBV) from Shanxi Province, and the association between mutations and disease related index...AIM: To investigate the frequency of mutations in pre-core (pre-C) and basic core promoter (BCP) regions of hepatitis B virus (HBV) from Shanxi Province, and the association between mutations and disease related indexes.METHODS: One hundred chronic hepatitis B patients treated at Shanxi Province Hospital of Traditional Chinese Medicine were included in this study. PCR-reverse dot blot hybridization and mismatch amplification mutation assay (MAMA)-PCR were used to detect the mutations in the HBV pre-C and BCP regions. HBV DNA content and liver function were compared between patients with mutant HBV pre-C and BCP loci and those with wild-type loci. The consistency between PCR-reverse dot blot hybridization and MAMA-PCR for detecting mutations in the HBV pre-C and BCP regions was assessed.RESULTS: Of the 100 serum samples detected, 9.38% had single mutations in the pre-C region, 29.17% had single mutations in the BCP region, 41.67% had mutations in both BCP and pre-C regions, and 19.79% had wild-type loci. The rates of BCP and pre-C mutations were 65.7% and 34.3%, respectively, in hepatitis B e antigen (HBeAg) positive patients, and 84.6% and 96.2%, respectively, in HBeAg negative patients. The rate of pre-C mutations was significantly higher in HBeAg negative patients than in HBeAg positive patients (χ<sup>2</sup> = 26.62, P = 0.00), but there was no significant difference in the distribution of mutations in the BCP region between HBeAg positive and negative patients (χ<sup>2</sup> = 2.43, P = 0.12). The presence of mutations in the pre-C (Wilcoxon W = 1802.5, P = 0.00) and BCP regions (Wilcoxon W = 2906.5, P = 0.00) was more common in patients with low HBV DNA content. Both AST and GGT were significantly higher in patients with mutant pre-C and BCP loci than in those with wild-type loci (P < 0.05). PCR-reverse dot blot hybridization and MAMA-PCR for detection of mutations in the BCP and pre-C regions had good consistency, and the Kappa values obtained were 0.91 and 0.58, respectively.CONCLU展开更多
研究三维空间机器人路径规划问题,由于系统求解时间较长、过早失去解的多样性、易陷入局部最优、个体适应度较差等问题,通过构建三维工作空间模型、引入变异算子和搜索无碰路径策略来解决,提出适宜于三维机器人路径规划的一种变异算子...研究三维空间机器人路径规划问题,由于系统求解时间较长、过早失去解的多样性、易陷入局部最优、个体适应度较差等问题,通过构建三维工作空间模型、引入变异算子和搜索无碰路径策略来解决,提出适宜于三维机器人路径规划的一种变异算子蚁群算法(Mutation Operator Ant Colony Algorithm,MOACA)。MOACA是一种关于模型构造的启发式搜索算法,算法在改进启发式函数设计、选择概率确定、信息素更新策略等基础上,引入逆转变异和插入变异算子,通过选择逆转点反序排列部分路径节点和随机插入路径节点的方法搜索无碰路径,对蚁群算法进行了局部优化改良。仿真结果表明,MOACA在搜索路径、收敛时间、适应度等方面较传统蚁群算法有明显改善,算法是有效可行的。展开更多
基金Supported by Youth Foundation of Health and Family Planning Commission of Shanxi ProvinceNo.201301024
文摘AIM: To investigate the frequency of mutations in pre-core (pre-C) and basic core promoter (BCP) regions of hepatitis B virus (HBV) from Shanxi Province, and the association between mutations and disease related indexes.METHODS: One hundred chronic hepatitis B patients treated at Shanxi Province Hospital of Traditional Chinese Medicine were included in this study. PCR-reverse dot blot hybridization and mismatch amplification mutation assay (MAMA)-PCR were used to detect the mutations in the HBV pre-C and BCP regions. HBV DNA content and liver function were compared between patients with mutant HBV pre-C and BCP loci and those with wild-type loci. The consistency between PCR-reverse dot blot hybridization and MAMA-PCR for detecting mutations in the HBV pre-C and BCP regions was assessed.RESULTS: Of the 100 serum samples detected, 9.38% had single mutations in the pre-C region, 29.17% had single mutations in the BCP region, 41.67% had mutations in both BCP and pre-C regions, and 19.79% had wild-type loci. The rates of BCP and pre-C mutations were 65.7% and 34.3%, respectively, in hepatitis B e antigen (HBeAg) positive patients, and 84.6% and 96.2%, respectively, in HBeAg negative patients. The rate of pre-C mutations was significantly higher in HBeAg negative patients than in HBeAg positive patients (χ<sup>2</sup> = 26.62, P = 0.00), but there was no significant difference in the distribution of mutations in the BCP region between HBeAg positive and negative patients (χ<sup>2</sup> = 2.43, P = 0.12). The presence of mutations in the pre-C (Wilcoxon W = 1802.5, P = 0.00) and BCP regions (Wilcoxon W = 2906.5, P = 0.00) was more common in patients with low HBV DNA content. Both AST and GGT were significantly higher in patients with mutant pre-C and BCP loci than in those with wild-type loci (P < 0.05). PCR-reverse dot blot hybridization and MAMA-PCR for detection of mutations in the BCP and pre-C regions had good consistency, and the Kappa values obtained were 0.91 and 0.58, respectively.CONCLU
文摘研究三维空间机器人路径规划问题,由于系统求解时间较长、过早失去解的多样性、易陷入局部最优、个体适应度较差等问题,通过构建三维工作空间模型、引入变异算子和搜索无碰路径策略来解决,提出适宜于三维机器人路径规划的一种变异算子蚁群算法(Mutation Operator Ant Colony Algorithm,MOACA)。MOACA是一种关于模型构造的启发式搜索算法,算法在改进启发式函数设计、选择概率确定、信息素更新策略等基础上,引入逆转变异和插入变异算子,通过选择逆转点反序排列部分路径节点和随机插入路径节点的方法搜索无碰路径,对蚁群算法进行了局部优化改良。仿真结果表明,MOACA在搜索路径、收敛时间、适应度等方面较传统蚁群算法有明显改善,算法是有效可行的。
