The emergence and prevalence of antibiotic resistance genes(ARGs) and pathogens in the environment are serious global health concern. However, information about the occurrence of ARGs and pathogens in recreational wat...The emergence and prevalence of antibiotic resistance genes(ARGs) and pathogens in the environment are serious global health concern. However, information about the occurrence of ARGs and pathogens in recreational water is still limited. Accordingly, we investigated the occurrence of six ARGs and human pathogens in three recreational lakes, and the correlations between ARGs and one mobile genetic element(intI1) were analyzed. The quantitative PCR results showed that the concentration of ARGs ranged from 4.58 × 10~0 to 5.0 × 10~5 copies/mL in water and from 5.78 × 10~3 to 5.89 × 10~8 copies/g dry weight(dw)in sediment. Sul1 exhibited the highest level among the five quantifiable ARGs. The concentrations of sul1, bla_(-TEM), and tetX exhibited significant positive correlations with intI1(p < 0.05), indicating that intI1 may be involved in their proliferation. The detection frequencies of ARGs ranged from 75%–10~0%, indicating the prevalence of these risks in this region. The concentration of Escherichia coli, Aeromonas spp., Mycobacterium avium,Pseudomonas aeruginosa, and Salmonella enterica ranged from 10~3 to 10~5 copies/10~0 mL in water and 10~4–10~6 copies/g dw in sediment. In total, 25% of the samples harbored all pathogen genes, indicating the prevalence of these pathogens in recreational lakes.Furthermore, the next-generation sequencing results showed that 68 genera of pathogens were present, among which Aeromonas, Mycobacterium, and Pseudomonas were the dominant ones in this region, posing a considerable potential health risk to public health. Overall, the widespread distribution of ARGs and pathogens underscores the need to better monitor and mitigate their propagation in recreational lakes and the associated risks to human health.展开更多
World heritage possesses various kinds of use value and non-use value. Measuring the recreational value of world heritage sites is a key issue in the heritage tourism. This paper employs Contingent Valuation Method (C...World heritage possesses various kinds of use value and non-use value. Measuring the recreational value of world heritage sites is a key issue in the heritage tourism. This paper employs Contingent Valuation Method (CVM), which is an evaluation instrument on the basis of tourists′ attitude and preference, to measure the recreational value of Jiuzhaigou, one of the World Natural Heritage sites in China, and analyzes the biases in the survey. The results show that: 1) In 2009, Jiuzhaigou′s recreational value was 3.46×108 yuan (RMB), and the per capita Willingness to Pay (WTP) was 137.31 yuan by CVM. Compared with the results of other methods, this result is significantly low. 2) Among the factors that influence Jiuzhaigou tourists′ WTP, psychological perception factors have the greatest effect while demographic and socio-economic characteristics have a small effect on WTP. 3) The virtuality in CVM creates biases both from CVM itself and in the procedures that CVM was implemented. The former includes hypothetical bias, information bias, protest response bias, and strategic bias; and the latter includes the questionnaire design, population and sample definition, sampling and data processing. The paper analyses those biases and illustrates their potential in-fluences on the accuracy of CVM measurement. 4) Although there are various defects in CVM measurement, it is still an effective valuation method.展开更多
Glaciers, with their unique and spectacular appearances and rich and varied terrain, have received widespread attention and become important tourist attractions. This paper uses the travel cost method to estimate the ...Glaciers, with their unique and spectacular appearances and rich and varied terrain, have received widespread attention and become important tourist attractions. This paper uses the travel cost method to estimate the recreational value of the glacier tourism resources of Yulong Snow Mountain(also called Jade Dragon Snow Mountain), which is the most developed glacier tourist attraction in China. First-hand information was obtained through field surveys, and the travel costs of visitors visiting the Yulong Snow Mountain glacier were calculated before the method was applied to evaluate the recreational value of the focal glacier resource. The results show that the Yulong Snow Mountain consumer surplus associated with its glacier resources in 2016 ranged from 645.59-3439.10 million CNY, and the total recreational value ranged from 1.97-8.17 billion CNY. Approaches allocating travel costs across multiple recreational sites, however, can vary, and there is large difference in estimated results depending on used approaches. Nevertheless, the results of the analysis can help understand the socio-economic value of glacier resources and provide a reference for their development and protection.展开更多
基金supported by the National Natural Science Foundation of China(No.51138006)the National Key Research on Water Environment Pollution Control in China(No.2012ZX07301-001)
文摘The emergence and prevalence of antibiotic resistance genes(ARGs) and pathogens in the environment are serious global health concern. However, information about the occurrence of ARGs and pathogens in recreational water is still limited. Accordingly, we investigated the occurrence of six ARGs and human pathogens in three recreational lakes, and the correlations between ARGs and one mobile genetic element(intI1) were analyzed. The quantitative PCR results showed that the concentration of ARGs ranged from 4.58 × 10~0 to 5.0 × 10~5 copies/mL in water and from 5.78 × 10~3 to 5.89 × 10~8 copies/g dry weight(dw)in sediment. Sul1 exhibited the highest level among the five quantifiable ARGs. The concentrations of sul1, bla_(-TEM), and tetX exhibited significant positive correlations with intI1(p < 0.05), indicating that intI1 may be involved in their proliferation. The detection frequencies of ARGs ranged from 75%–10~0%, indicating the prevalence of these risks in this region. The concentration of Escherichia coli, Aeromonas spp., Mycobacterium avium,Pseudomonas aeruginosa, and Salmonella enterica ranged from 10~3 to 10~5 copies/10~0 mL in water and 10~4–10~6 copies/g dw in sediment. In total, 25% of the samples harbored all pathogen genes, indicating the prevalence of these pathogens in recreational lakes.Furthermore, the next-generation sequencing results showed that 68 genera of pathogens were present, among which Aeromonas, Mycobacterium, and Pseudomonas were the dominant ones in this region, posing a considerable potential health risk to public health. Overall, the widespread distribution of ARGs and pathogens underscores the need to better monitor and mitigate their propagation in recreational lakes and the associated risks to human health.
基金Under the auspices of Sino-British Fellowship by the British Academy (No. SG-47266)National Natural Science Foundation of China (No. 40371030)
文摘World heritage possesses various kinds of use value and non-use value. Measuring the recreational value of world heritage sites is a key issue in the heritage tourism. This paper employs Contingent Valuation Method (CVM), which is an evaluation instrument on the basis of tourists′ attitude and preference, to measure the recreational value of Jiuzhaigou, one of the World Natural Heritage sites in China, and analyzes the biases in the survey. The results show that: 1) In 2009, Jiuzhaigou′s recreational value was 3.46×108 yuan (RMB), and the per capita Willingness to Pay (WTP) was 137.31 yuan by CVM. Compared with the results of other methods, this result is significantly low. 2) Among the factors that influence Jiuzhaigou tourists′ WTP, psychological perception factors have the greatest effect while demographic and socio-economic characteristics have a small effect on WTP. 3) The virtuality in CVM creates biases both from CVM itself and in the procedures that CVM was implemented. The former includes hypothetical bias, information bias, protest response bias, and strategic bias; and the latter includes the questionnaire design, population and sample definition, sampling and data processing. The paper analyses those biases and illustrates their potential in-fluences on the accuracy of CVM measurement. 4) Although there are various defects in CVM measurement, it is still an effective valuation method.
基金supported by the National Natural Science Foundation of China (Grant No.41690143)the Fund from the State Key Laboratory of Cryospheric Sciences,Chinese Academy of Sciences (Grant No.SKLCS-OP-201602)the Fundamental Research Funds for the Central Universities from Nanjing Agricultural University,China (Grant No.SK2016033)
文摘Glaciers, with their unique and spectacular appearances and rich and varied terrain, have received widespread attention and become important tourist attractions. This paper uses the travel cost method to estimate the recreational value of the glacier tourism resources of Yulong Snow Mountain(also called Jade Dragon Snow Mountain), which is the most developed glacier tourist attraction in China. First-hand information was obtained through field surveys, and the travel costs of visitors visiting the Yulong Snow Mountain glacier were calculated before the method was applied to evaluate the recreational value of the focal glacier resource. The results show that the Yulong Snow Mountain consumer surplus associated with its glacier resources in 2016 ranged from 645.59-3439.10 million CNY, and the total recreational value ranged from 1.97-8.17 billion CNY. Approaches allocating travel costs across multiple recreational sites, however, can vary, and there is large difference in estimated results depending on used approaches. Nevertheless, the results of the analysis can help understand the socio-economic value of glacier resources and provide a reference for their development and protection.
