The response of wheat plants to different osmotic stress levels varied among the different organs root, shoot and spike and the situation of these organs with application of two Cu++ levels 5 mM and 25 mM as CuSO4. Th...The response of wheat plants to different osmotic stress levels varied among the different organs root, shoot and spike and the situation of these organs with application of two Cu++ levels 5 mM and 25 mM as CuSO4. The sensitivity of root organ was related with reduction in fresh, dry matter and length. This resulted from reduction of soluble sugar reflected a reduction in water uptake and K+ content of the cell sap. In the moderate organ spike, the reduction in fresh, dry matter and length were concomitant with the accumulation of soluble sugar and a huge accumulation of soluble protein. In the higher organ shoot, this related with more water uptake which in turn induced an accumulation of soluble protein and cofactor K+ content. It can be recorded that shoot was higher Na+ accumulation than root and spike. Data also showed further stimulatory effect on growth parameters by Cu++ applications with either concentration (7.5 mM and 25 mM). Irrigating the soil with either 7.5 or 25 mM CuSO4 induced a huge accumulation in soluble sugar, soluble protein and nitrate reductase. Cupper treatment with either concentration 7.5 mM or 25 mM induced a marked decrease in Na+ content at all OSL and has no significant change in the accumulation of K+ in both shoot and spike whereas induced a huge accumulation in root organ. The synthesis of protein bands with molecular weight 32.3 KDa at -1.5 MPa NaCl level treated with either 7.5 mM or 25 mM Cu++ concentrations was induced. Also the appearance of protein band with molecular weight 37KDa induced only at Cu++ treatments with 25 mM concentration in both control and under different osmotic levels (0.0, -0.3 MPa, -0.9 MPa, -1.5 MPa NaCl).展开更多
Ⅰ. INTRODUCTION The survival of motoneurons in vertebrates during their embryonic period depends on certain neurotrophic substances provided from their skeletal muscles. Skeletal muscles have been shown, in in vitro ...Ⅰ. INTRODUCTION The survival of motoneurons in vertebrates during their embryonic period depends on certain neurotrophic substances provided from their skeletal muscles. Skeletal muscles have been shown, in in vitro and in vivo experiments, to contain substances which are capable of enhancing the survival and development of motoneurons but preventing the展开更多
目的对圆柏花粉浸液40kD条带的蛋白组分进行初步鉴定,明确其一级结构。方法将圆柏花粉浸液使用SDS-PAGE初步分离后,取40kD条带进行质谱分析。结果 40kD条带中获得肽段分别与Jun v 1,Cup a 1,Jun a 2及其他植物蛋白序列一致。结论 40kD...目的对圆柏花粉浸液40kD条带的蛋白组分进行初步鉴定,明确其一级结构。方法将圆柏花粉浸液使用SDS-PAGE初步分离后,取40kD条带进行质谱分析。结果 40kD条带中获得肽段分别与Jun v 1,Cup a 1,Jun a 2及其他植物蛋白序列一致。结论 40kD条带可能包含多种成分,包括柏科花粉1组2组变应原等。展开更多
文摘The response of wheat plants to different osmotic stress levels varied among the different organs root, shoot and spike and the situation of these organs with application of two Cu++ levels 5 mM and 25 mM as CuSO4. The sensitivity of root organ was related with reduction in fresh, dry matter and length. This resulted from reduction of soluble sugar reflected a reduction in water uptake and K+ content of the cell sap. In the moderate organ spike, the reduction in fresh, dry matter and length were concomitant with the accumulation of soluble sugar and a huge accumulation of soluble protein. In the higher organ shoot, this related with more water uptake which in turn induced an accumulation of soluble protein and cofactor K+ content. It can be recorded that shoot was higher Na+ accumulation than root and spike. Data also showed further stimulatory effect on growth parameters by Cu++ applications with either concentration (7.5 mM and 25 mM). Irrigating the soil with either 7.5 or 25 mM CuSO4 induced a huge accumulation in soluble sugar, soluble protein and nitrate reductase. Cupper treatment with either concentration 7.5 mM or 25 mM induced a marked decrease in Na+ content at all OSL and has no significant change in the accumulation of K+ in both shoot and spike whereas induced a huge accumulation in root organ. The synthesis of protein bands with molecular weight 32.3 KDa at -1.5 MPa NaCl level treated with either 7.5 mM or 25 mM Cu++ concentrations was induced. Also the appearance of protein band with molecular weight 37KDa induced only at Cu++ treatments with 25 mM concentration in both control and under different osmotic levels (0.0, -0.3 MPa, -0.9 MPa, -1.5 MPa NaCl).
文摘Ⅰ. INTRODUCTION The survival of motoneurons in vertebrates during their embryonic period depends on certain neurotrophic substances provided from their skeletal muscles. Skeletal muscles have been shown, in in vitro and in vivo experiments, to contain substances which are capable of enhancing the survival and development of motoneurons but preventing the
文摘目的对圆柏花粉浸液40kD条带的蛋白组分进行初步鉴定,明确其一级结构。方法将圆柏花粉浸液使用SDS-PAGE初步分离后,取40kD条带进行质谱分析。结果 40kD条带中获得肽段分别与Jun v 1,Cup a 1,Jun a 2及其他植物蛋白序列一致。结论 40kD条带可能包含多种成分,包括柏科花粉1组2组变应原等。