AIM： To investigate the expression and clinical significance of Wnt member 5a （Wnt5a） and receptor tyrosine kinase-like orphan receptor 2 （Ror2） in hepatocellular carcinoma （HCC）.METHODS： In HCC tissues obtain...AIM： To investigate the expression and clinical significance of Wnt member 5a （Wnt5a） and receptor tyrosine kinase-like orphan receptor 2 （Ror2） in hepatocellular carcinoma （HCC）.METHODS： In HCC tissues obtained from 85 patients, the protein expressions of Wnt5a, Rot2, 13-catenin, and Ki-67 via immunohistochemical staining using the Envision Plus System. The antibody binding was visualized with 3, 3＇-diaminobenzidine tetrahydrochloride （DAB） before brief counterstaining with Mayer＇s hematoxylin. The degree of immunohistochemical staining was recorded using a semiquantitative and subjective grading system. The mRNA expression of Rot2 was examined by real time reverse transcription polymerase chain reaction, including nineteen of the 85 HCC and three normal liver tissues. The ratios of Ror2 to the housekeeping gene GAPDH represented the normalized relative levels of Ror2 expression. To determine the prognostic factor, the outcome of the 82 patients was determined by reviewing their medical charts. The overall and diseasefree survival rates were estimated using the Kaplan-Meier method and compared with the logrank test. The prognostic analysis was carried out with univariate and multivariate Cox regressions models.RESULTS： Compared to nontumorous （hepatitis or cirrhotic） tissues, Rot2 mRNA expression was clearly decreased in HCC. Rot2 and Wnt5a protein expressions in the majority of HCC patients （63% and 77%, respectively） was significantly less in tumor tissues, as compared to adjacent nontumorous tissues, and this reduction was correlated with increasing serum α-fetoprotein and tumor stage. In 68% （58/85） of the HCC cases, the expression of IB-catenin in tumor tissues was either downregulated in the cellular membrane, upregulated in the cytoplasm, or both. Survival analysis indicated that Wnt5a and Ror2 protein expressions could be regarded as independent prognostic factors for HCC; HCC patients with decreased Wnt5a or Ror2 protein expression had a poorer prognosis than thos展开更多
目的:探讨神经重症昏迷患者不良预后的相关因素。方法:采用回顾性调查方法,纳入2017年1月至2018年4月在重庆医科大学附属第一医院住院治疗的昏迷患者[格拉斯哥昏迷评分(Glasgow coma score,GCS)≤8分]共117例,出院时根据格拉斯哥预后评...目的:探讨神经重症昏迷患者不良预后的相关因素。方法:采用回顾性调查方法,纳入2017年1月至2018年4月在重庆医科大学附属第一医院住院治疗的昏迷患者[格拉斯哥昏迷评分(Glasgow coma score,GCS)≤8分]共117例,出院时根据格拉斯哥预后评分(Glasgow outcome score,GOS)将患者分为生存组(74例)和死亡组(43例)。由专人对入选患者病历进行回顾性分析,分析资料包括:病史资料(性别、年龄、诊断、器官功能不全个数),病情评估资料(GCS评分、急性生理与慢性健康评分APACHEⅡ评分),脑电图指标(发病72 h内脑电图模式、基本背景、波幅特点、通过脑电图背景及波幅特点进行Synek分级、反应性、睡眠波),72 h内生化指标(Na^+、K^+、Cl^-、Ca^2+、白蛋白、乳酸等)。结果:Logistic回归分析显示脑电图反应性OR值为4.717,95%CI=1.333~16.686;年龄OR值为1.057,95%CI=1.020~1.095;APACHEⅡ评分OR值为1.201,95%CI=1.082~1.334;GCS评分OR值为4.705,95%CI=1.322~16.743(均P<0.05)。结论:脑电图反应性、年龄、APACHEⅡ评分、GCS评分均与神经重症昏迷患者急性期死亡风险具有相关性,临床上应密切关注该类指标变化,以便及时、客观、全面对患者进行预后判定。展开更多
【目的】探讨雄激素受体（AR）、人体抑癌基因p53在三阴性乳腺癌（triple-negative breast cancer，TNBC）中的表达及其意义。【方法】收集2010年1月至2011年12月本院诊治的87例TNBC患者（三阴组）和231例非TNBC患者（非三阴组）的临床...【目的】探讨雄激素受体（AR）、人体抑癌基因p53在三阴性乳腺癌（triple-negative breast cancer，TNBC）中的表达及其意义。【方法】收集2010年1月至2011年12月本院诊治的87例TNBC患者（三阴组）和231例非TNBC患者（非三阴组）的临床资料，采用免疫组化法检测两组AR、p53的表达，并分析其与临床病理特征和预后的关系。【结果】三阴组患者p53和AR的阳性表达率分别为49．43％（43／87）和37．93％（33／87），明显高于非三阴组的33．77％（78／231）和27．3％（63／231），其差异均有统计学意义（χ2=9．451，P=0．025；χ2=8．287，P=0．031）；AR阳性表达与淋巴结转移相关（P〈0．05）；p53阳性表达与组织学分级和分子类型相关（P〈0．05），而其他指标与p53、AR阳性表达均不相关（P〉0．05）；三阴组患者5年局部复发率和远处转移率显著高于非三阴组患者（P〈0．05），5年生存率显著低于非三阴组患者（P〈0．05）。【结论】TNBC中p53和AR的表达上调，与肿瘤细胞分化程度、淋巴结转移状态相关；p53和AR可作为乳腺癌患者预后的预测指标。展开更多
Introduction: Developing countries are characterized by a high maternal mortality rate, particulary related to the management of childbirth. The author describes in this work 588 childbirth labors that took place with...Introduction: Developing countries are characterized by a high maternal mortality rate, particulary related to the management of childbirth. The author describes in this work 588 childbirth labors that took place without any medical supervision. Method: All patients who reached the hospital with a full cervix dilation were included in the study. The outcomes of those childbirth labors without medical supervision were evaluated at the maternal and neonatal level. Results and Discussion: The average age of the patients was 28.1 ± 13 years with 47% nulliparous and 30% pauciparous. These patients represented 14% of all births;59% of the patients had had three and five prenatal consultations. 71% of them came straight from home and had meconium-stained amniotic fluid. The APGAR score was greater than 6 in 94% of newborns, and 66.7 of them weighed between 2500 and 3500 g. Only 0.9% of patients coming from home needed a caesarean section. Conclusion: Home birth is not yet possible in Africa because it is not supervised by professionals who know the risks of childbirth, its complications and recognize the warning signs;however, the results of this preliminary study show that the issue of home childbirth in Côte d’Ivoire can be reconsidered subject to greater involvement of medical staffs.展开更多
基金Supported by The fund of Nature Science Foundation of Shandong Province,No.ZR2009CM041
文摘AIM： To investigate the expression and clinical significance of Wnt member 5a （Wnt5a） and receptor tyrosine kinase-like orphan receptor 2 （Ror2） in hepatocellular carcinoma （HCC）.METHODS： In HCC tissues obtained from 85 patients, the protein expressions of Wnt5a, Rot2, 13-catenin, and Ki-67 via immunohistochemical staining using the Envision Plus System. The antibody binding was visualized with 3, 3＇-diaminobenzidine tetrahydrochloride （DAB） before brief counterstaining with Mayer＇s hematoxylin. The degree of immunohistochemical staining was recorded using a semiquantitative and subjective grading system. The mRNA expression of Rot2 was examined by real time reverse transcription polymerase chain reaction, including nineteen of the 85 HCC and three normal liver tissues. The ratios of Ror2 to the housekeeping gene GAPDH represented the normalized relative levels of Ror2 expression. To determine the prognostic factor, the outcome of the 82 patients was determined by reviewing their medical charts. The overall and diseasefree survival rates were estimated using the Kaplan-Meier method and compared with the logrank test. The prognostic analysis was carried out with univariate and multivariate Cox regressions models.RESULTS： Compared to nontumorous （hepatitis or cirrhotic） tissues, Rot2 mRNA expression was clearly decreased in HCC. Rot2 and Wnt5a protein expressions in the majority of HCC patients （63% and 77%, respectively） was significantly less in tumor tissues, as compared to adjacent nontumorous tissues, and this reduction was correlated with increasing serum α-fetoprotein and tumor stage. In 68% （58/85） of the HCC cases, the expression of IB-catenin in tumor tissues was either downregulated in the cellular membrane, upregulated in the cytoplasm, or both. Survival analysis indicated that Wnt5a and Ror2 protein expressions could be regarded as independent prognostic factors for HCC; HCC patients with decreased Wnt5a or Ror2 protein expression had a poorer prognosis than thos
文摘【目的】探讨雄激素受体（AR）、人体抑癌基因p53在三阴性乳腺癌（triple-negative breast cancer，TNBC）中的表达及其意义。【方法】收集2010年1月至2011年12月本院诊治的87例TNBC患者（三阴组）和231例非TNBC患者（非三阴组）的临床资料，采用免疫组化法检测两组AR、p53的表达，并分析其与临床病理特征和预后的关系。【结果】三阴组患者p53和AR的阳性表达率分别为49．43％（43／87）和37．93％（33／87），明显高于非三阴组的33．77％（78／231）和27．3％（63／231），其差异均有统计学意义（χ2=9．451，P=0．025；χ2=8．287，P=0．031）；AR阳性表达与淋巴结转移相关（P〈0．05）；p53阳性表达与组织学分级和分子类型相关（P〈0．05），而其他指标与p53、AR阳性表达均不相关（P〉0．05）；三阴组患者5年局部复发率和远处转移率显著高于非三阴组患者（P〈0．05），5年生存率显著低于非三阴组患者（P〈0．05）。【结论】TNBC中p53和AR的表达上调，与肿瘤细胞分化程度、淋巴结转移状态相关；p53和AR可作为乳腺癌患者预后的预测指标。
文摘Introduction: Developing countries are characterized by a high maternal mortality rate, particulary related to the management of childbirth. The author describes in this work 588 childbirth labors that took place without any medical supervision. Method: All patients who reached the hospital with a full cervix dilation were included in the study. The outcomes of those childbirth labors without medical supervision were evaluated at the maternal and neonatal level. Results and Discussion: The average age of the patients was 28.1 ± 13 years with 47% nulliparous and 30% pauciparous. These patients represented 14% of all births;59% of the patients had had three and five prenatal consultations. 71% of them came straight from home and had meconium-stained amniotic fluid. The APGAR score was greater than 6 in 94% of newborns, and 66.7 of them weighed between 2500 and 3500 g. Only 0.9% of patients coming from home needed a caesarean section. Conclusion: Home birth is not yet possible in Africa because it is not supervised by professionals who know the risks of childbirth, its complications and recognize the warning signs;however, the results of this preliminary study show that the issue of home childbirth in Côte d’Ivoire can be reconsidered subject to greater involvement of medical staffs.
