The management of diabetic wounds remains a critical therapeutic challenge. Platelet-rich plasma (PRP) gel, PRP-derived exosomes (PRP-Exos), and mesenchymal stem cell-derived exosomes (MSC-Exos) have demonstrated ther...The management of diabetic wounds remains a critical therapeutic challenge. Platelet-rich plasma (PRP) gel, PRP-derived exosomes (PRP-Exos), and mesenchymal stem cell-derived exosomes (MSC-Exos) have demonstrated therapeutic potential in wound treatment. Unfortunately, their poor mechanical properties, the short half-lives of growth factors (GFs), and the burst release of GFs and exosomes have limited their clinical applications. Furthermore, proteases in diabetic wounds degrade GFs, which hampers wound repair. Silk fibroin is an enzyme-immobilization biomaterial that could protect GFs from proteases. Herein, we developed novel dual-crosslinked hydrogels based on silk protein (SP) (sericin and fibroin), including SP@PRP, SP@MSC-Exos, and SP@PRP-Exos, to promote diabetic wound healing synergistically. SP@PRP was prepared from PRP and SP using calcium gluconate/thrombin as agonist, while SP@PRP-Exos and SP@MSC-Exos were derived from exosomes and SP with genipin as crosslinker. SP provided improved mechanical properties and enabled the sustained release of GFs and exosomes, thereby overcoming the limitations of PRP and exosomes in wound healing. The dual-crosslinked hydrogels displayed shear-induced thinning, self-healing, and eradication of microbial biofilms in a bone-mimicking environment. In vivo, the dual-crosslinked hydrogels contributed to faster diabetic wound healing than PRP and SP by upregulating GFs expression, down-regulating matrix metalloproteinase-9 expression, and by promoting an anti-NETotic effect, angiogenesis, and re-epithelialization. Hence, these dual-crosslinked hydrogels have the potential to be translated into a new generation of diabetic wound dressings.展开更多
Platelet-rich plasma(PRP)can stimulate the proliferation of stem cells and have a positive effect on tissue repair.Although many commercialized PRP preparation kits are already on the market,first-line clinical worker...Platelet-rich plasma(PRP)can stimulate the proliferation of stem cells and have a positive effect on tissue repair.Although many commercialized PRP preparation kits are already on the market,first-line clinical workers are still not satisfied with most of the PRP kits.The work of commercial PRP kits is based on the density of blood elements.However,the blood elements are very close in density which makes the separation challenging.Therefore,the mentioned commercialized kits are generally contaminated by leucocytes and erythrocyte.In this study,a home-designed PRP device was developed to use a separation membrane with adequate cut-off pore size of 5μm,3μm and 2μm for the groups of H5M,H3M,and H2M,respectively,to be placed in the middle of the centrifuge tube.The home-designed H2M showed a very promising results regardless of the final volume(1.82±0.09 ml),platelet yield(8.39±0.44%),Red Blood Cells(0%),White Blood Cells(0%),and Relative Concentration of Platelet Increment value(225.09%).Further,it showed a good result in cell viability and cytotoxicity and confirmed a good multilineage potentials.The concentration in PRP prepared by group H2M was relatively stable and far above average.All the fibrin fibers were linked together as bridging strands or strings and turned into an inter-connected porous structure for nutrients transportation and regenerative cell migration.We believe that the home-designed group H2M should have a great potential to develop into the final product to meet the requirements of first-line clinical workers.展开更多
Military drill injury is a significant part of military medical research.The increase of training intensity and changes in training methods lead to differences in injury types.The ideal therapeutic modality should all...Military drill injury is a significant part of military medical research.The increase of training intensity and changes in training methods lead to differences in injury types.The ideal therapeutic modality should allow rapid healing at a reasonable cost and minimize impact on patients’life.Platelet-rich plasma(PRP),a platelet concentrate,is rich in a variety of growth factors and widely used clinically as a minimally invasive treatment.It plays an important role in injury repair and rehabilitation.In this article,we review the therapeutic role of PRP in military drill injury and its possible underlying mechanisms,with a focus on plantar fasciitis,stress fractures and other common injuries,in order to provide basic support for military reserve.展开更多
[目的]比较自体富血小板血浆(platelet-rich plasma,PRP)与糖皮质激素甲基强的松龙(methylprednisolone,MPS)局部注射治疗肱骨外上髁炎的疗效。[方法]2016年1月~2019年3月,本院骨科就诊的64例肱骨外上髁炎患者纳入本研究,随机分为两组,...[目的]比较自体富血小板血浆(platelet-rich plasma,PRP)与糖皮质激素甲基强的松龙(methylprednisolone,MPS)局部注射治疗肱骨外上髁炎的疗效。[方法]2016年1月~2019年3月,本院骨科就诊的64例肱骨外上髁炎患者纳入本研究,随机分为两组,每组各32例。分别给予PRP和MPS痛点局部注射治疗。比较两组患者的疼痛视觉评分(visual an⁃alogue scale,VAS)、Mayo肘关节功能评分(Mayo elbow performance score,MEPS)和肩臂手残障(disabilities of the arm,shoul⁃der and hand,DASH)评分。[结果]治疗过程中两组患者均未发生严重不良反应。随时间推移,两组患者VAS和DASH评分显著减少(P<0.05),而MEPS评分显著增加(P<0.05)。治疗前两组患者VAS评分、MEPS评分和DASH评分的差异均无统计学意义(P>0.05)。治疗后1、3个月时PRP组VAS评分和DASH评分高于MPS组,MEPS评分低于MPS组,差异有统计学意义(P<0.05)。治疗后6、12个月时,PRP组VAS评分和DASH评分低于MPS组,而MEPS评分高于MPS组,差异有统计学意义(P<0.05)。[结论]局部注射自体PRP和糖皮质激素治疗肱骨外上髁炎早期均能缓解疼痛,改善肘关节功能,而糖皮质激素治疗后期逐渐失效,PRP治疗效果逐渐提升。展开更多
基金supported by the National Natural Science Foundation of China(51973076)the Fundamental Research Funds for Central Universities(2020kfyXJJS035).
文摘The management of diabetic wounds remains a critical therapeutic challenge. Platelet-rich plasma (PRP) gel, PRP-derived exosomes (PRP-Exos), and mesenchymal stem cell-derived exosomes (MSC-Exos) have demonstrated therapeutic potential in wound treatment. Unfortunately, their poor mechanical properties, the short half-lives of growth factors (GFs), and the burst release of GFs and exosomes have limited their clinical applications. Furthermore, proteases in diabetic wounds degrade GFs, which hampers wound repair. Silk fibroin is an enzyme-immobilization biomaterial that could protect GFs from proteases. Herein, we developed novel dual-crosslinked hydrogels based on silk protein (SP) (sericin and fibroin), including SP@PRP, SP@MSC-Exos, and SP@PRP-Exos, to promote diabetic wound healing synergistically. SP@PRP was prepared from PRP and SP using calcium gluconate/thrombin as agonist, while SP@PRP-Exos and SP@MSC-Exos were derived from exosomes and SP with genipin as crosslinker. SP provided improved mechanical properties and enabled the sustained release of GFs and exosomes, thereby overcoming the limitations of PRP and exosomes in wound healing. The dual-crosslinked hydrogels displayed shear-induced thinning, self-healing, and eradication of microbial biofilms in a bone-mimicking environment. In vivo, the dual-crosslinked hydrogels contributed to faster diabetic wound healing than PRP and SP by upregulating GFs expression, down-regulating matrix metalloproteinase-9 expression, and by promoting an anti-NETotic effect, angiogenesis, and re-epithelialization. Hence, these dual-crosslinked hydrogels have the potential to be translated into a new generation of diabetic wound dressings.
基金This work was supported by the Indonesia Endowment Fund for Education(LPDP)within the ministry of Finance,Indonesia(grant number PRJ-317/LPDP.3/2016).
文摘Platelet-rich plasma(PRP)can stimulate the proliferation of stem cells and have a positive effect on tissue repair.Although many commercialized PRP preparation kits are already on the market,first-line clinical workers are still not satisfied with most of the PRP kits.The work of commercial PRP kits is based on the density of blood elements.However,the blood elements are very close in density which makes the separation challenging.Therefore,the mentioned commercialized kits are generally contaminated by leucocytes and erythrocyte.In this study,a home-designed PRP device was developed to use a separation membrane with adequate cut-off pore size of 5μm,3μm and 2μm for the groups of H5M,H3M,and H2M,respectively,to be placed in the middle of the centrifuge tube.The home-designed H2M showed a very promising results regardless of the final volume(1.82±0.09 ml),platelet yield(8.39±0.44%),Red Blood Cells(0%),White Blood Cells(0%),and Relative Concentration of Platelet Increment value(225.09%).Further,it showed a good result in cell viability and cytotoxicity and confirmed a good multilineage potentials.The concentration in PRP prepared by group H2M was relatively stable and far above average.All the fibrin fibers were linked together as bridging strands or strings and turned into an inter-connected porous structure for nutrients transportation and regenerative cell migration.We believe that the home-designed group H2M should have a great potential to develop into the final product to meet the requirements of first-line clinical workers.
基金supported by the National Natural Science Foundation of China(81671924 and 81272105)the Science and Technology Key Project of Guangdong Province(2014B020212010)+1 种基金the National Key Research and Development Plan of China(2017YFC1103301)the Military Medical Innovation Special Projects(18CXZ029)。
文摘Military drill injury is a significant part of military medical research.The increase of training intensity and changes in training methods lead to differences in injury types.The ideal therapeutic modality should allow rapid healing at a reasonable cost and minimize impact on patients’life.Platelet-rich plasma(PRP),a platelet concentrate,is rich in a variety of growth factors and widely used clinically as a minimally invasive treatment.It plays an important role in injury repair and rehabilitation.In this article,we review the therapeutic role of PRP in military drill injury and its possible underlying mechanisms,with a focus on plantar fasciitis,stress fractures and other common injuries,in order to provide basic support for military reserve.
文摘[目的]比较自体富血小板血浆(platelet-rich plasma,PRP)与糖皮质激素甲基强的松龙(methylprednisolone,MPS)局部注射治疗肱骨外上髁炎的疗效。[方法]2016年1月~2019年3月,本院骨科就诊的64例肱骨外上髁炎患者纳入本研究,随机分为两组,每组各32例。分别给予PRP和MPS痛点局部注射治疗。比较两组患者的疼痛视觉评分(visual an⁃alogue scale,VAS)、Mayo肘关节功能评分(Mayo elbow performance score,MEPS)和肩臂手残障(disabilities of the arm,shoul⁃der and hand,DASH)评分。[结果]治疗过程中两组患者均未发生严重不良反应。随时间推移,两组患者VAS和DASH评分显著减少(P<0.05),而MEPS评分显著增加(P<0.05)。治疗前两组患者VAS评分、MEPS评分和DASH评分的差异均无统计学意义(P>0.05)。治疗后1、3个月时PRP组VAS评分和DASH评分高于MPS组,MEPS评分低于MPS组,差异有统计学意义(P<0.05)。治疗后6、12个月时,PRP组VAS评分和DASH评分低于MPS组,而MEPS评分高于MPS组,差异有统计学意义(P<0.05)。[结论]局部注射自体PRP和糖皮质激素治疗肱骨外上髁炎早期均能缓解疼痛,改善肘关节功能,而糖皮质激素治疗后期逐渐失效,PRP治疗效果逐渐提升。
