The heat shock transcription factors （HSFs） are the major heat shock factors regulating the heat stress response. They participate in regulating the expression of heat shock proteins （HSPs）, which are critical in ...The heat shock transcription factors （HSFs） are the major heat shock factors regulating the heat stress response. They participate in regulating the expression of heat shock proteins （HSPs）, which are critical in the protection against stress damage and many other important biological processes. Study of the HSF gene family is important for understanding the mechanism by which plants respond to stress. The completed genome sequences of rice （Oryza sativa） and Arabidopsis （Arabidopsis thaliana） constitute a valuable resource for comparative genomic analysis, as they are representatives of the two major evolutionary lineages within the angiosperms： the monocotyledons and the dicotyledons. The identification of phylogenefic relationships among HSF proteins in these species is a fundamental step to unravel the functionality of new and yet uncharacterized genes belonging to this family.In this study, the full complement of HSF genes in rice and Arabidopsis has probably been identified through the genome-wide scan. Phylogenetic analyses resulted in the identification of three major clusters of orthologous genes that contain members belonging to both species, which must have been represented in their common ancestor before the taxonomic splitting of the angiosperms. Fttrther analysis of the phylogenetic tree reveals a possible dicot specific gene group. We also identified nine pairs of paralogs, as evidence for studies on the evolution history of rice HSF family and rice genome evolution. Expression data analysis indicates that HSF proteins are widely expressed in plants. These results provide a solid base for future functional genomic studies of the HSF gene family in rice and Arabidopsis.展开更多
Several TCP genes have been reported to play Important roles in plant development; the TCP homologs encode a plant-specific family of putative transcription factors. To understand the evolutionary relationship of TCP ...Several TCP genes have been reported to play Important roles in plant development; the TCP homologs encode a plant-specific family of putative transcription factors. To understand the evolutionary relationship of TCP genes of Arabidopsis thallana and Oryza sativa L. （hereafter called rice）, we have identified 23 and 22 TCP genes in the Arabidopsis and rice genomea, respectively. Using phylogenetic analysis, we grouped these TCP genes into three classes. In addition, the motifs outside the TCP domain further support the evolutionary relationships among these genes. The genome distribution of the TCP genes strongly supports the hypothesis that genome-wlde and tandem duplication contributed to the expansion of the TCP gene family. The expression pattern of the TCP genes was analyzed further, providing useful clues about the function of these genes.展开更多
文摘The heat shock transcription factors （HSFs） are the major heat shock factors regulating the heat stress response. They participate in regulating the expression of heat shock proteins （HSPs）, which are critical in the protection against stress damage and many other important biological processes. Study of the HSF gene family is important for understanding the mechanism by which plants respond to stress. The completed genome sequences of rice （Oryza sativa） and Arabidopsis （Arabidopsis thaliana） constitute a valuable resource for comparative genomic analysis, as they are representatives of the two major evolutionary lineages within the angiosperms： the monocotyledons and the dicotyledons. The identification of phylogenefic relationships among HSF proteins in these species is a fundamental step to unravel the functionality of new and yet uncharacterized genes belonging to this family.In this study, the full complement of HSF genes in rice and Arabidopsis has probably been identified through the genome-wide scan. Phylogenetic analyses resulted in the identification of three major clusters of orthologous genes that contain members belonging to both species, which must have been represented in their common ancestor before the taxonomic splitting of the angiosperms. Fttrther analysis of the phylogenetic tree reveals a possible dicot specific gene group. We also identified nine pairs of paralogs, as evidence for studies on the evolution history of rice HSF family and rice genome evolution. Expression data analysis indicates that HSF proteins are widely expressed in plants. These results provide a solid base for future functional genomic studies of the HSF gene family in rice and Arabidopsis.
基金Supported by Funds from the National Key Basic Research Developments Program of the Ministry of Science and Technology, China （2006CB101700, 2005CB120802）, the High-Tech Research and Development （863） Programe of China （2005AA2710330）, the Shuguang Scholarship （04SG15）, and the Shanghai Institutes of Biological Sciences （Reproductive Development Project）. Publication of this paper is supported by the National Natural Science Foundation of China （30624808）.
文摘Several TCP genes have been reported to play Important roles in plant development; the TCP homologs encode a plant-specific family of putative transcription factors. To understand the evolutionary relationship of TCP genes of Arabidopsis thallana and Oryza sativa L. （hereafter called rice）, we have identified 23 and 22 TCP genes in the Arabidopsis and rice genomea, respectively. Using phylogenetic analysis, we grouped these TCP genes into three classes. In addition, the motifs outside the TCP domain further support the evolutionary relationships among these genes. The genome distribution of the TCP genes strongly supports the hypothesis that genome-wlde and tandem duplication contributed to the expansion of the TCP gene family. The expression pattern of the TCP genes was analyzed further, providing useful clues about the function of these genes.
