Objective To isolate, incubate, and identify 4-chlorophenol-degrading complex bacteria, determine the tolerance of these bacteria to phenolic derivatives and study their synergetic metabolism as well as the aboriginal...Objective To isolate, incubate, and identify 4-chlorophenol-degrading complex bacteria, determine the tolerance of these bacteria to phenolic derivatives and study their synergetic metabolism as well as the aboriginal microbes and co-metabolic degradation of mixed chlorophenols in river water. Methods Microbial community of complex bacteria was identified by plate culture observation techniques and Gram stain method. Bacterial growth inhibition test was used to determine the tolerance of complex bacteria to toxicants. Biodegradability of phenolic derivatives was determined by adding 4-chlorophenol-degrading bacteria in river water. Results The complex bacteria were identified as Mycopiana, Alcaligenes, Pseudomonas, and Flavobacterium. The domesticated complex bacteria were more tolerant to phenolic derivatives than the aboriginal bacteria from Qinhuai River. The biodegradability of chlorophenols, dihydroxybenzenes and nitrophenols under various aquatic conditions was determined and compared. The complex bacteria exhibited a higher metabolic efficiency on chemicals than the aboriginal microbes, and the final removal rate of phenolic derivatives was increased at least by 55% when the complex bacteria were added into river water. The metabolic relationship between dominant mixed bacteria and river bacteria was studied. Conclusion The complex bacteria domesticated by 4-chlorophenol can grow and be metabolized to take other chlorophenols, dihydroxybenzenes and nitrophenols as the sole carbon and energy source. There is a synergetic metabolism of most compounds between the aboriginal microbes in river water and the domesticated complex bacteria, 4- chlorophenol-degrading bacteria can co-metabolize various chlorophenols in fiver water.展开更多
The interplay between melatonin and ethylene in the regulation of fruit metabolism and the underlying molecular mechanism of this interplay remain largely unclear.Here,widely targeted metabolomics analysis revealed a ...The interplay between melatonin and ethylene in the regulation of fruit metabolism and the underlying molecular mechanism of this interplay remain largely unclear.Here,widely targeted metabolomics analysis revealed a total of 464 metabolites present in berry skin.Among them,27 significantly differentially accumulated metabolites(DAMs)were produced in response to melatonin treatment in the presence or absence of 1-MCP.Most of the DAMs were secondary metabolites,including flavonoids,phenolic acids,stilbenes,and flavonols.Additionally,the accumulation of 25 DAMs was regulated by melatonin via ethylene.RNA-seq analysis indicated that melatonin primarily regulated the pathways of plant hormone signal transduction and secondary metabolite biosynthesis via ethylene.Gene-metabolite association analysis showed that melatonin regulated the expression of the VvSTS1,VvF3H,VvLAR2,and VvDFR genes,suggesting that these genes may play key roles in regulating secondary metabolites in the skin;additionally,VvMYB14 and VvACS1 were suggested to be involved in the regulation of secondary metabolites.Further experiments revealed that melatonin induced the expression of VvMYB14 and that VvMYB14 increased ethylene production by transcriptionally activating VvACS1,thereby affecting the accumulation of secondary metabolites.Collectively,melatonin promotes ethylene biosynthesis and alters secondary metabolite accumulation through the regulation of VvACS1 by VvMYB14.展开更多
This study aimed to better understand the beneficial effect of oat phenolic compounds(OPC)in improving metabolic syndrome via regulating metabolites and gut microbiota composition.The oral administration of OPC can al...This study aimed to better understand the beneficial effect of oat phenolic compounds(OPC)in improving metabolic syndrome via regulating metabolites and gut microbiota composition.The oral administration of OPC can alleviate a range of metabolic syndromes in mice caused by high-fat dietary feeding,such as weight gain,glucose intolerance,elevated serum lipid levels(TC,TG,HDL-C,and LDL-C)and oxidative stress(GSH-Px,T-AOC,SOD and MDA)as well as adipocyte hypertrophy.Besides,OPC-treated mice also have reduced chronic inflammation,which indicates that OPC can interfere with the expression of genes related to glycolipid metabolism.Furthermore,HFD-fed mice can cause an imbalance in gut microbiota,while the addition of OPC can improve this negative effect,which also further demonstrated the importance of gut microbiota in the regulation of metabolic disorders.The OPC significantly increased the abundance of Bacteroidetes and reduced the diversity of Firmicutes(p<0.05)compared with the HFD-fed mice.OPC treatment rebuilt gut microbiota composition via increasing Eubacterium levels and reducing numbers of Alistipes and Lachnospiracea e NK4A136 groups in HFD-fed mice.This result also provided a potential explanation for polyphenols benefit from whole grains in glycolipid metabolism disorders.展开更多
基金supported by 973 Program (No. 2008CB418203)New Century Excellent Talents in University (No. 05-0481)
文摘Objective To isolate, incubate, and identify 4-chlorophenol-degrading complex bacteria, determine the tolerance of these bacteria to phenolic derivatives and study their synergetic metabolism as well as the aboriginal microbes and co-metabolic degradation of mixed chlorophenols in river water. Methods Microbial community of complex bacteria was identified by plate culture observation techniques and Gram stain method. Bacterial growth inhibition test was used to determine the tolerance of complex bacteria to toxicants. Biodegradability of phenolic derivatives was determined by adding 4-chlorophenol-degrading bacteria in river water. Results The complex bacteria were identified as Mycopiana, Alcaligenes, Pseudomonas, and Flavobacterium. The domesticated complex bacteria were more tolerant to phenolic derivatives than the aboriginal bacteria from Qinhuai River. The biodegradability of chlorophenols, dihydroxybenzenes and nitrophenols under various aquatic conditions was determined and compared. The complex bacteria exhibited a higher metabolic efficiency on chemicals than the aboriginal microbes, and the final removal rate of phenolic derivatives was increased at least by 55% when the complex bacteria were added into river water. The metabolic relationship between dominant mixed bacteria and river bacteria was studied. Conclusion The complex bacteria domesticated by 4-chlorophenol can grow and be metabolized to take other chlorophenols, dihydroxybenzenes and nitrophenols as the sole carbon and energy source. There is a synergetic metabolism of most compounds between the aboriginal microbes in river water and the domesticated complex bacteria, 4- chlorophenol-degrading bacteria can co-metabolize various chlorophenols in fiver water.
基金the National Key R&D Program of China(2018YFD1000200)the Key Research and Development Program of Shandong Province(2019GNC106149)+2 种基金the National Natural Science Foundation of China(31872068)the Funds of Shandong“Double Tops”Program(SYL2017YSTD10)Shandong Province Government(SDAIT-06-03).
文摘The interplay between melatonin and ethylene in the regulation of fruit metabolism and the underlying molecular mechanism of this interplay remain largely unclear.Here,widely targeted metabolomics analysis revealed a total of 464 metabolites present in berry skin.Among them,27 significantly differentially accumulated metabolites(DAMs)were produced in response to melatonin treatment in the presence or absence of 1-MCP.Most of the DAMs were secondary metabolites,including flavonoids,phenolic acids,stilbenes,and flavonols.Additionally,the accumulation of 25 DAMs was regulated by melatonin via ethylene.RNA-seq analysis indicated that melatonin primarily regulated the pathways of plant hormone signal transduction and secondary metabolite biosynthesis via ethylene.Gene-metabolite association analysis showed that melatonin regulated the expression of the VvSTS1,VvF3H,VvLAR2,and VvDFR genes,suggesting that these genes may play key roles in regulating secondary metabolites in the skin;additionally,VvMYB14 and VvACS1 were suggested to be involved in the regulation of secondary metabolites.Further experiments revealed that melatonin induced the expression of VvMYB14 and that VvMYB14 increased ethylene production by transcriptionally activating VvACS1,thereby affecting the accumulation of secondary metabolites.Collectively,melatonin promotes ethylene biosynthesis and alters secondary metabolite accumulation through the regulation of VvACS1 by VvMYB14.
基金supported by National Natural Science Foundation of China(31601476)Zhejiang Provincial Natural Science Foundation(LY21C200005).
文摘This study aimed to better understand the beneficial effect of oat phenolic compounds(OPC)in improving metabolic syndrome via regulating metabolites and gut microbiota composition.The oral administration of OPC can alleviate a range of metabolic syndromes in mice caused by high-fat dietary feeding,such as weight gain,glucose intolerance,elevated serum lipid levels(TC,TG,HDL-C,and LDL-C)and oxidative stress(GSH-Px,T-AOC,SOD and MDA)as well as adipocyte hypertrophy.Besides,OPC-treated mice also have reduced chronic inflammation,which indicates that OPC can interfere with the expression of genes related to glycolipid metabolism.Furthermore,HFD-fed mice can cause an imbalance in gut microbiota,while the addition of OPC can improve this negative effect,which also further demonstrated the importance of gut microbiota in the regulation of metabolic disorders.The OPC significantly increased the abundance of Bacteroidetes and reduced the diversity of Firmicutes(p<0.05)compared with the HFD-fed mice.OPC treatment rebuilt gut microbiota composition via increasing Eubacterium levels and reducing numbers of Alistipes and Lachnospiracea e NK4A136 groups in HFD-fed mice.This result also provided a potential explanation for polyphenols benefit from whole grains in glycolipid metabolism disorders.
