本实验主要是研究低温保存后两个品种水仙(Narcissus tazetta var. chinensis)叶片和花瓣的解剖学结构。通过低温贮藏的两个品种水仙的三年生鳞茎,其生长开花时间延迟,在非正常开花季节进行常规水培;再利用石蜡切片技术,研究其在幼嫩期...本实验主要是研究低温保存后两个品种水仙(Narcissus tazetta var. chinensis)叶片和花瓣的解剖学结构。通过低温贮藏的两个品种水仙的三年生鳞茎,其生长开花时间延迟,在非正常开花季节进行常规水培;再利用石蜡切片技术,研究其在幼嫩期、生长期和成熟期三个阶段的花瓣、叶片解剖学结构,与矮壮素处理的材料进行比较。研究发现,经过低温贮藏后,两个品种花瓣和叶片的薄壁组织和维管束在生长期和成熟期的变化与矮壮素处理的材料有差异,薄壁细胞解体提前,花瓣维管束、叶片小维管束出现木质部延伸现象,半周木维管束明显。研究低温贮藏对水仙花瓣与叶片的解剖学结构的影响,能为进一步应用生长延缓剂于水仙上以改变和延长水仙花期提供理论依据。展开更多
Firstly the petal of Crocus sativus L was cultured on the medium that supplemented with different combinations of hormones. The petal-like structures(PLS) were induced on medium, but the induction rates were different...Firstly the petal of Crocus sativus L was cultured on the medium that supplemented with different combinations of hormones. The petal-like structures(PLS) were induced on medium, but the induction rates were different in various medium. The highest induction rate of petal-like structures was obtained on the media that was supplemented with NAA (4 mg/L) and KT (8 mg/L). The petal-like structures were subcultured on another media when the structure was produced on the explants and proliferate groups. The later media was used for inducing style-stigma-like structures(SSLS). The induction rate of style-stigma-like-structures in the petal-like structures group is much higher than the rate in the preceding work, and the maximum of style-stigma-like structures produced per explant was 30. The best result of style-stigma-like structures was observed on the petal-like structure groups which came from the third treatment. The differentiation rate of style-stigma-like structures is stable in the subcultures of petal-like structures. The result revealed that the induction frequency of style-stigma-like structures formed on the petal-like structures is higher than that form on the petals of C.sativus L.展开更多
文摘本实验主要是研究低温保存后两个品种水仙(Narcissus tazetta var. chinensis)叶片和花瓣的解剖学结构。通过低温贮藏的两个品种水仙的三年生鳞茎,其生长开花时间延迟,在非正常开花季节进行常规水培;再利用石蜡切片技术,研究其在幼嫩期、生长期和成熟期三个阶段的花瓣、叶片解剖学结构,与矮壮素处理的材料进行比较。研究发现,经过低温贮藏后,两个品种花瓣和叶片的薄壁组织和维管束在生长期和成熟期的变化与矮壮素处理的材料有差异,薄壁细胞解体提前,花瓣维管束、叶片小维管束出现木质部延伸现象,半周木维管束明显。研究低温贮藏对水仙花瓣与叶片的解剖学结构的影响,能为进一步应用生长延缓剂于水仙上以改变和延长水仙花期提供理论依据。
文摘Firstly the petal of Crocus sativus L was cultured on the medium that supplemented with different combinations of hormones. The petal-like structures(PLS) were induced on medium, but the induction rates were different in various medium. The highest induction rate of petal-like structures was obtained on the media that was supplemented with NAA (4 mg/L) and KT (8 mg/L). The petal-like structures were subcultured on another media when the structure was produced on the explants and proliferate groups. The later media was used for inducing style-stigma-like structures(SSLS). The induction rate of style-stigma-like-structures in the petal-like structures group is much higher than the rate in the preceding work, and the maximum of style-stigma-like structures produced per explant was 30. The best result of style-stigma-like structures was observed on the petal-like structure groups which came from the third treatment. The differentiation rate of style-stigma-like structures is stable in the subcultures of petal-like structures. The result revealed that the induction frequency of style-stigma-like structures formed on the petal-like structures is higher than that form on the petals of C.sativus L.
