AIM: To investigate the alterations in both structure and contractile responsiveness of ocular ciliary artery(OCA) in spontaneously hypertensive rat(SHR).METHODS: In this experiment, 20-week-old male SHR and Wistar Ky...AIM: To investigate the alterations in both structure and contractile responsiveness of ocular ciliary artery(OCA) in spontaneously hypertensive rat(SHR).METHODS: In this experiment, 20-week-old male SHR and Wistar Kyoto rat(WKY) were studied. The heart rate(HR), the blood pressure(BP; the systolic BP and the diastolic BP) of rats with an electronic sphygmomanometer were measured. Vascular morphometry and isometric tension measurement were used to investigate the alterations in structure and contractility of OCA.RESULTS: A general narrowing of OCAs was observed in SHR compared to the control WYK. In SHR, the media of OCAs were thicker, the luminal diameters were smaller, and the media-to-lumen ratios were higher when compared with WKY(P<0.05). The contractions of OCAs evoked by norepinephrine were smaller in SHR compared to control(P<0.05). Then, OCAs were pretreated with iberiotoxin, L-NAME, or indomethacin 30 min before norepinephrineinduced contraction. Iberiotoxin(0.1 μmol/L) has not changed the norepinephrine-induced contractions in OCAs from both groups. However, L-NAME(100 μmol/L) increased the vasoconstrictions, the increased extents were similar in SHR and WKY(P>0.05). Indomethacin(10 μmol/L) decreased the contractions induced by norepinephrine in OCAs from WKY(P<0.05), but did not change those contractions in vessels from SHR(P>0.05).CONCLUSION: Our results demonstrate that the structure and function of OCAs are altered in hypertension. OCAs from SHR are remodeled with decreased lumen diameter and increased media-to-lumen ratio. Moreover, the contractile responsiveness of OCAs from SHR is diminished due to the disruption of vasoconstrictive effect of prostaglandins.展开更多
目的探讨青光眼小梁切除手术对眼表结构及泪膜的影响。方法选择66例80只急性闭角型青光眼行小梁切除的患者,观察术前及术后6个月的角膜表面规则指数(SRI)、角膜表面非对称性指数(SAI)、角膜荧光素染色及虎红染色、基础泪液分泌试验(Schi...目的探讨青光眼小梁切除手术对眼表结构及泪膜的影响。方法选择66例80只急性闭角型青光眼行小梁切除的患者,观察术前及术后6个月的角膜表面规则指数(SRI)、角膜表面非对称性指数(SAI)、角膜荧光素染色及虎红染色、基础泪液分泌试验(Schirmer I test)、泪膜破裂时间(BUT),并对数据进行统计学分析。结果小梁切除术前,术后1周、1个月、3个月和6个月的SRI为(0.26±0.11)、(0.65±0.30)、(0.53±0.25)、(0.45±0.17)和(0.28±0.12),SAI为(0.42±0.22)、(0.78±0.23)、(0.68±0.26)、(0.60±0.22)、(0.46±0.21),术后3个月内的SRI及SAI明显增加,术后1周最明显,与术前相比差异有统计学意义(P<0.05),术后6个月SRI及SAI与术前相比差异无统计学意义(P>0.05);术后1周、1个月、3个月角膜荧光素染色及虎红染色点增多,泪液分泌量明显减少、BUT明显缩短,与术前相比差异有统计学意义(P<0.05),术后6个月角膜荧光素染色、虎红染色、SIt及BUT恢复至术前水平,与术前比差异无统计学意义(P>0.05)。结论青光眼小梁切除术对眼表结构和泪膜具有一定程度的影响,这可能与多种因素有关,应积极采取预防措施。展开更多
基金Supported by the Natural Science Foundation of China(No.81100695)the Japan Society for the Promotion of Science(JSPS)([C] 25462750)
文摘AIM: To investigate the alterations in both structure and contractile responsiveness of ocular ciliary artery(OCA) in spontaneously hypertensive rat(SHR).METHODS: In this experiment, 20-week-old male SHR and Wistar Kyoto rat(WKY) were studied. The heart rate(HR), the blood pressure(BP; the systolic BP and the diastolic BP) of rats with an electronic sphygmomanometer were measured. Vascular morphometry and isometric tension measurement were used to investigate the alterations in structure and contractility of OCA.RESULTS: A general narrowing of OCAs was observed in SHR compared to the control WYK. In SHR, the media of OCAs were thicker, the luminal diameters were smaller, and the media-to-lumen ratios were higher when compared with WKY(P<0.05). The contractions of OCAs evoked by norepinephrine were smaller in SHR compared to control(P<0.05). Then, OCAs were pretreated with iberiotoxin, L-NAME, or indomethacin 30 min before norepinephrineinduced contraction. Iberiotoxin(0.1 μmol/L) has not changed the norepinephrine-induced contractions in OCAs from both groups. However, L-NAME(100 μmol/L) increased the vasoconstrictions, the increased extents were similar in SHR and WKY(P>0.05). Indomethacin(10 μmol/L) decreased the contractions induced by norepinephrine in OCAs from WKY(P<0.05), but did not change those contractions in vessels from SHR(P>0.05).CONCLUSION: Our results demonstrate that the structure and function of OCAs are altered in hypertension. OCAs from SHR are remodeled with decreased lumen diameter and increased media-to-lumen ratio. Moreover, the contractile responsiveness of OCAs from SHR is diminished due to the disruption of vasoconstrictive effect of prostaglandins.
文摘目的探讨青光眼小梁切除手术对眼表结构及泪膜的影响。方法选择66例80只急性闭角型青光眼行小梁切除的患者,观察术前及术后6个月的角膜表面规则指数(SRI)、角膜表面非对称性指数(SAI)、角膜荧光素染色及虎红染色、基础泪液分泌试验(Schirmer I test)、泪膜破裂时间(BUT),并对数据进行统计学分析。结果小梁切除术前,术后1周、1个月、3个月和6个月的SRI为(0.26±0.11)、(0.65±0.30)、(0.53±0.25)、(0.45±0.17)和(0.28±0.12),SAI为(0.42±0.22)、(0.78±0.23)、(0.68±0.26)、(0.60±0.22)、(0.46±0.21),术后3个月内的SRI及SAI明显增加,术后1周最明显,与术前相比差异有统计学意义(P<0.05),术后6个月SRI及SAI与术前相比差异无统计学意义(P>0.05);术后1周、1个月、3个月角膜荧光素染色及虎红染色点增多,泪液分泌量明显减少、BUT明显缩短,与术前相比差异有统计学意义(P<0.05),术后6个月角膜荧光素染色、虎红染色、SIt及BUT恢复至术前水平,与术前比差异无统计学意义(P>0.05)。结论青光眼小梁切除术对眼表结构和泪膜具有一定程度的影响,这可能与多种因素有关,应积极采取预防措施。
