As a new type of methane control agent, natural plant extract has been widely studied in recent years,but in vivo studies are few. This study was to investigate the effects of the dietary supplementation of 2 differen...As a new type of methane control agent, natural plant extract has been widely studied in recent years,but in vivo studies are few. This study was to investigate the effects of the dietary supplementation of 2 different polyphenols on the methane（CH4） emission and digestion metabolism in sheep. Ten healthy crossbred sheep（Dorper（?）× small-tailed Han ♀; BW 60.0 ± 1.73 kg） were used in a change-over design.The sheep were fed the following 3 diets in the present study: the basal diet（CON） with no supplementation; the basal diet supplemented with 2 g mulberry leaf flavonoid（MLF） per day per sheep; the basal diet supplemented with 0.25 g resveratrol（RES） per day per sheep. Both MLF and RES reduced CH4 emission scaled to metabolic weight per kilogram of DMI and CO2 output scaled to metabolic weight, but the effect of RES was significant（P < 0.05）. Both MLF and RES significantly improved apparent digestibility of DM, OM, NDF, ADF, and nitrogen, but the effect of RES was significant（P < 0.05）. Both MLF and RES significantly improved ME（P < 0.05） and reduced energy losses in CH4 emission（P > 0.05）. In conclusion, MLF and RES can improve the digestibility of nutrients, the utilization of nutrients and energy, and reduce CH4 emission, but they are not conducive to nitrogen retention.展开更多
目的优化浊点萃取协同超声波辅助提取桑叶黄酮工艺,并对其降血脂作用进行研究。方法以桑叶黄酮得率为指标,采用单因素和响应面实验,优化浊点萃取协同超声波辅助法的提取工艺条件。同时将小鼠随机分为桑叶黄酮低、中、高剂量组、正常对...目的优化浊点萃取协同超声波辅助提取桑叶黄酮工艺,并对其降血脂作用进行研究。方法以桑叶黄酮得率为指标,采用单因素和响应面实验,优化浊点萃取协同超声波辅助法的提取工艺条件。同时将小鼠随机分为桑叶黄酮低、中、高剂量组、正常对照组、模型对照组和辛伐他汀组。除正常对照组外,其余5组喂养60%高脂饲料构建高血脂小鼠模型。测定小鼠的体重、血清中血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)和高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)含量及肝组织中超氧化物歧化酶(superoxide dismutase,SOD)活性、丙二醛(malondialdehyde,MDA)含量和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性。结果最佳提取工艺条件为:十二烷基硫酸钠添加量2.1%、超声波功率400W、超声时间42min,此时桑叶黄酮得率2.82%,此法的提取效果优于其他两种单一的提取方式。桑叶黄酮对高血脂小鼠降血脂实验结果得出:桑叶黄酮能有效抑制高血脂小鼠体重的增加,减少高血脂小鼠血清中TC、TG、LDL-C的含量,降低高血脂小鼠肝组织中MDA含量,提高GSH-Px和SOD活性,表明桑叶黄酮对高血脂小鼠具有良好的降血脂作用。结论优化后的提取条件能够有效提取桑叶黄酮,得到的桑叶黄酮具有较好的降血脂作用,该研究可为桑叶黄酮的提取及开发应用提供参考。展开更多
基金supported by the earmarked fund for the Modern Agro-Industry Technology Research System,China(CARS-39,200903006)To Build Healthy Farming Model and Demonstrate for Sheep(2012BAD39B05-3)
文摘As a new type of methane control agent, natural plant extract has been widely studied in recent years,but in vivo studies are few. This study was to investigate the effects of the dietary supplementation of 2 different polyphenols on the methane（CH4） emission and digestion metabolism in sheep. Ten healthy crossbred sheep（Dorper（?）× small-tailed Han ♀; BW 60.0 ± 1.73 kg） were used in a change-over design.The sheep were fed the following 3 diets in the present study: the basal diet（CON） with no supplementation; the basal diet supplemented with 2 g mulberry leaf flavonoid（MLF） per day per sheep; the basal diet supplemented with 0.25 g resveratrol（RES） per day per sheep. Both MLF and RES reduced CH4 emission scaled to metabolic weight per kilogram of DMI and CO2 output scaled to metabolic weight, but the effect of RES was significant（P < 0.05）. Both MLF and RES significantly improved apparent digestibility of DM, OM, NDF, ADF, and nitrogen, but the effect of RES was significant（P < 0.05）. Both MLF and RES significantly improved ME（P < 0.05） and reduced energy losses in CH4 emission（P > 0.05）. In conclusion, MLF and RES can improve the digestibility of nutrients, the utilization of nutrients and energy, and reduce CH4 emission, but they are not conducive to nitrogen retention.
文摘目的优化浊点萃取协同超声波辅助提取桑叶黄酮工艺,并对其降血脂作用进行研究。方法以桑叶黄酮得率为指标,采用单因素和响应面实验,优化浊点萃取协同超声波辅助法的提取工艺条件。同时将小鼠随机分为桑叶黄酮低、中、高剂量组、正常对照组、模型对照组和辛伐他汀组。除正常对照组外,其余5组喂养60%高脂饲料构建高血脂小鼠模型。测定小鼠的体重、血清中血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)和高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)含量及肝组织中超氧化物歧化酶(superoxide dismutase,SOD)活性、丙二醛(malondialdehyde,MDA)含量和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性。结果最佳提取工艺条件为:十二烷基硫酸钠添加量2.1%、超声波功率400W、超声时间42min,此时桑叶黄酮得率2.82%,此法的提取效果优于其他两种单一的提取方式。桑叶黄酮对高血脂小鼠降血脂实验结果得出:桑叶黄酮能有效抑制高血脂小鼠体重的增加,减少高血脂小鼠血清中TC、TG、LDL-C的含量,降低高血脂小鼠肝组织中MDA含量,提高GSH-Px和SOD活性,表明桑叶黄酮对高血脂小鼠具有良好的降血脂作用。结论优化后的提取条件能够有效提取桑叶黄酮,得到的桑叶黄酮具有较好的降血脂作用,该研究可为桑叶黄酮的提取及开发应用提供参考。
