Fields experiments were conducted at the Department of Crop Protection, Faculty of Agriculture, University of Maiduguri Teaching and Research Farm, Maiduguri during 2010 and 2011 cropping seasons to investigate the ef...Fields experiments were conducted at the Department of Crop Protection, Faculty of Agriculture, University of Maiduguri Teaching and Research Farm, Maiduguri during 2010 and 2011 cropping seasons to investigate the effects of intercropping pattern on stem borer pest infestation in pearl millet (Pennisetum glaucum L.) intercropped with ground nut (Arachis hypogea L.). A split-plot design was used to test the intercrop pattern of 1:0 ratio (sole millet), 1:1 ratio (1 millet row to 1 ground nut row), 2:1 ratio (2 millet rows to 1 ground nut row) and 1:2 ratio (1 millet row to 2 ground nut rows). The results obtained showed that the intercrop pattern of 1:2 ratio and 1:1 ratio yielded less stem borer infestation and abundance in pearl millet, and as well supported high panicle weight and grain yield. In addition to recommending either of these two intercropping patterns to pearl millet farmers for more effective stem borer pest management, results further show the need for identification of effective intercropping patterns in other cropping systems.展开更多
The 2.2 kb EcoRI fragment containing the chloroplast pshA gene from millet (Setaria italica) has been cloned and the nucleotide sequence of the 5’-noncoding region has been determined. The 5’-flanking region is foun...The 2.2 kb EcoRI fragment containing the chloroplast pshA gene from millet (Setaria italica) has been cloned and the nucleotide sequence of the 5’-noncoding region has been determined. The 5’-flanking region is found to contain prokaryote-like promoter structures: compared with prokaryotic promoters, the“-35”box (TTGACA) shows 100% homology while, in the ‘-10’box (TATACT), one different nucleotide is found. In addition, between these two boxes, there is a consensus sequence“TATATA”.just as in prokaryotic promoters. All these results indicate that millet psbA promoter has both prokaryotic and eukaryotic characteristies. The mRNA leader region of millet pshA gene is 87 bp, the same length as sorghum. However, an additional CTATTT sequence is found as compared with rice and an additional TTTT, as with wheat, barley and rye. So the differences between C3 and C4 plants may be universal in the family of Gramineae. Furthermore, computer analysis shows that a small stem-loop structure might be formed in pshA mRNA leader region in these six plants. The above-mentioned additional CTATTT sequence happens to be just located within the stem-loop structure, thus leading to different sizes of the stem-loops among these six species. It is likely that this small secondary structure may have some effect on the expression and regulation of the psbA gene.展开更多
文摘Fields experiments were conducted at the Department of Crop Protection, Faculty of Agriculture, University of Maiduguri Teaching and Research Farm, Maiduguri during 2010 and 2011 cropping seasons to investigate the effects of intercropping pattern on stem borer pest infestation in pearl millet (Pennisetum glaucum L.) intercropped with ground nut (Arachis hypogea L.). A split-plot design was used to test the intercrop pattern of 1:0 ratio (sole millet), 1:1 ratio (1 millet row to 1 ground nut row), 2:1 ratio (2 millet rows to 1 ground nut row) and 1:2 ratio (1 millet row to 2 ground nut rows). The results obtained showed that the intercrop pattern of 1:2 ratio and 1:1 ratio yielded less stem borer infestation and abundance in pearl millet, and as well supported high panicle weight and grain yield. In addition to recommending either of these two intercropping patterns to pearl millet farmers for more effective stem borer pest management, results further show the need for identification of effective intercropping patterns in other cropping systems.
文摘The 2.2 kb EcoRI fragment containing the chloroplast pshA gene from millet (Setaria italica) has been cloned and the nucleotide sequence of the 5’-noncoding region has been determined. The 5’-flanking region is found to contain prokaryote-like promoter structures: compared with prokaryotic promoters, the“-35”box (TTGACA) shows 100% homology while, in the ‘-10’box (TATACT), one different nucleotide is found. In addition, between these two boxes, there is a consensus sequence“TATATA”.just as in prokaryotic promoters. All these results indicate that millet psbA promoter has both prokaryotic and eukaryotic characteristies. The mRNA leader region of millet pshA gene is 87 bp, the same length as sorghum. However, an additional CTATTT sequence is found as compared with rice and an additional TTTT, as with wheat, barley and rye. So the differences between C3 and C4 plants may be universal in the family of Gramineae. Furthermore, computer analysis shows that a small stem-loop structure might be formed in pshA mRNA leader region in these six plants. The above-mentioned additional CTATTT sequence happens to be just located within the stem-loop structure, thus leading to different sizes of the stem-loops among these six species. It is likely that this small secondary structure may have some effect on the expression and regulation of the psbA gene.
