MicroRNAs are small single-stranded RNA molecules consisting of approximately 22 nucleotides (nt), and have post-transcriptional regulatory functions. By gene chip screening, we previously showed that miR-142-3p was s...MicroRNAs are small single-stranded RNA molecules consisting of approximately 22 nucleotides (nt), and have post-transcriptional regulatory functions. By gene chip screening, we previously showed that miR-142-3p was significantly upregulated in colorectal cancer tissue and was associated with clinicopathological features, compared with matched non-tumor tissue. In this study, we confirmed significant upregulation of miR-142-3p in 60 colorectal cancer samples and three colorectal cancer cell lines by quantitative real-time PCR (qRT-PCR). Using software and network resources, we predicted TCF7 as a target of miR-142-3p, which we confirmed with dual-luciferase assays. By RT-PCR and Western blot analysis, we found miR-142-3p negatively regulates TCF7 expression post-transcriptionally. CCK8 assays and growth curves indicated that overexpression of miR-142-3p in SW480 colorectal cancer cells potently inhibited cell proliferation in vitro. The expression of TCF7 mRNA and protein was upregulated in both colorectal cancer tissues and colorectal cancer cell lines, closely correlating with its function as an oncogene in promoting tumor cell proliferation and inhibiting apoptosis. With TCF7 as a direct target, our results suggested that miR-142-3p may be involved in the regulation of cell proliferation in colorectal cancer.展开更多
目的检测妊娠期糖尿病(GDM)患者血清微小RNA(miR)⁃142⁃3p、miR⁃497⁃5p表达水平,分析其对GDM的诊断价值。方法选取2019年6月-2022年6月在河南中医药大学第五临床医学院妇产科行产前检查的142例GDM孕妇作为GDM组,另外随机选择一般资料与GD...目的检测妊娠期糖尿病(GDM)患者血清微小RNA(miR)⁃142⁃3p、miR⁃497⁃5p表达水平,分析其对GDM的诊断价值。方法选取2019年6月-2022年6月在河南中医药大学第五临床医学院妇产科行产前检查的142例GDM孕妇作为GDM组,另外随机选择一般资料与GDM组匹配的142名正常糖耐量孕妇作为对照组。比较两组孕妇血清miR⁃142⁃3p、miR⁃497⁃5p水平,使用受试者工作特征(ROC)曲线分析血清miR⁃142⁃3p、miR⁃497⁃5p水平对孕妇发生GDM的评估价值。结果与对照组比较,GDM组患者miR⁃142⁃3p水平、有糖尿病史占比、经产妇占比、空腹血糖(FPG)、餐后2 h血糖(2 h PG)、空腹胰岛素(FINS)、糖化血红蛋白(HbA1c)、胰岛素抵抗指数(HOMA⁃IR)升高,血清miR⁃497⁃5p水平显著降低,差异均有统计学意义(t=8.303、6.818、9.551、8.781、9.054、6.464、9.475、11.671、7.883,P均<0.05)。miR⁃142⁃3p、miR⁃497⁃5p联合检测预测孕妇发生GDM的ROC曲线下面积(AUC)为0.838,敏感性为73.24%,特异性为82.39%,优于各自单独诊断(Z=4.379、3.170,P均<0.01)。结论GDM患者血清miR⁃142⁃3p表达显著上调、miR⁃497⁃5p表达显著下调,二者联合对孕妇是否发生GDM有较好的预测价值。展开更多
文摘MicroRNAs are small single-stranded RNA molecules consisting of approximately 22 nucleotides (nt), and have post-transcriptional regulatory functions. By gene chip screening, we previously showed that miR-142-3p was significantly upregulated in colorectal cancer tissue and was associated with clinicopathological features, compared with matched non-tumor tissue. In this study, we confirmed significant upregulation of miR-142-3p in 60 colorectal cancer samples and three colorectal cancer cell lines by quantitative real-time PCR (qRT-PCR). Using software and network resources, we predicted TCF7 as a target of miR-142-3p, which we confirmed with dual-luciferase assays. By RT-PCR and Western blot analysis, we found miR-142-3p negatively regulates TCF7 expression post-transcriptionally. CCK8 assays and growth curves indicated that overexpression of miR-142-3p in SW480 colorectal cancer cells potently inhibited cell proliferation in vitro. The expression of TCF7 mRNA and protein was upregulated in both colorectal cancer tissues and colorectal cancer cell lines, closely correlating with its function as an oncogene in promoting tumor cell proliferation and inhibiting apoptosis. With TCF7 as a direct target, our results suggested that miR-142-3p may be involved in the regulation of cell proliferation in colorectal cancer.
文摘目的检测妊娠期糖尿病(GDM)患者血清微小RNA(miR)⁃142⁃3p、miR⁃497⁃5p表达水平,分析其对GDM的诊断价值。方法选取2019年6月-2022年6月在河南中医药大学第五临床医学院妇产科行产前检查的142例GDM孕妇作为GDM组,另外随机选择一般资料与GDM组匹配的142名正常糖耐量孕妇作为对照组。比较两组孕妇血清miR⁃142⁃3p、miR⁃497⁃5p水平,使用受试者工作特征(ROC)曲线分析血清miR⁃142⁃3p、miR⁃497⁃5p水平对孕妇发生GDM的评估价值。结果与对照组比较,GDM组患者miR⁃142⁃3p水平、有糖尿病史占比、经产妇占比、空腹血糖(FPG)、餐后2 h血糖(2 h PG)、空腹胰岛素(FINS)、糖化血红蛋白(HbA1c)、胰岛素抵抗指数(HOMA⁃IR)升高,血清miR⁃497⁃5p水平显著降低,差异均有统计学意义(t=8.303、6.818、9.551、8.781、9.054、6.464、9.475、11.671、7.883,P均<0.05)。miR⁃142⁃3p、miR⁃497⁃5p联合检测预测孕妇发生GDM的ROC曲线下面积(AUC)为0.838,敏感性为73.24%,特异性为82.39%,优于各自单独诊断(Z=4.379、3.170,P均<0.01)。结论GDM患者血清miR⁃142⁃3p表达显著上调、miR⁃497⁃5p表达显著下调,二者联合对孕妇是否发生GDM有较好的预测价值。
