摘要
目的探讨甲状腺组织和外周血单个核细胞中微小RNA-142-3p(miR-142-3p)、微小RNA-150(miR-150)在桥本甲状腺炎(HT)中的表达水平及临床意义。方法选取2017年3月至2019年6月南阳市中心医院行甲状腺手术切除并经病理确诊为HT病人87例为HT组;同期选取南阳市中心医院行甲状腺腺瘤切除术的非癌病人73例为对照组。采用实时荧光定量PCR(qRT-PCR)法检测两组甲状腺组织和外周血单个核细胞中miR-142-3p、miR-150表达水平;分析HF病人miR-142-3p、miR-150水平与甲状腺功能参数及相关细胞因子相关性。结果HT组甲状腺组织和外周血单个核细胞中miR-142-3p[(1.54±0.32)、(2.25±0.54)]比[(1.00±0.23)、(1.00±0.24)]、miR-150[(2.21±0.43)、(3.78±1.14)]比[(1.00±0.16)、(1.00±0.19)]表达水平均显著高于对照组(P<0.05);两组甲状腺功能参数及相关细胞因子水平比较,均差异有统计学意义,HT组促甲状腺激素(TSH)、甲状腺过氧化物酶抗体(TPOAb)、白细胞介素17(IL-17)、白细胞介素6(IL-6)、干扰素-γ(IFN-γ)、转化生长因子β1(TGF-β1)水平显著高于对照组(P<0.05),游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)水平显著低于对照组(P<0.05);HT病人甲状腺组织和外周血单个核细胞中miR-142-3p、miR-150水平呈正相关,且与TSH、TPOAb、IL-17、IL-6、IFN-γ、TGF-β1呈正相关,与FT3、FT4呈负相关(P<0.05)。结论甲状腺组织和外周血单个核细胞中miR-142-3p、miR-150水平的变化与HT有关。miR-142-3p、miR-150对评价HT的早期危险性、降低患病率有重要意义。
ObjectiveTo detect the expression levels of microRNA-142-3 p(miR-142-3 p)and microRNA-150(miR-150)in thyroid tissues and peripheral blood mononuclear cells of Hashimoto’s thyroiditis(HT)patients,and to explore the clinical significance of their expression levels in HT patients.MethodsA total of 87 patients,who were hospitalized in Nanyang Central Hospital for thyroidecto-my and diagnosed as HT by pathology from March 2017 to June 2019,were selected as the study objects(HT group).And 73 cases of non-cancer patients,who underwent thyroidectomy in Nanyang Central Hospital during the same time,were selected as the control group(control group).The expression levels of miR-142-3 p and miR-150 in thyroid tissues and peripheral blood mononuclear cells were detected by real-time fluorescence quantitative PCR(qRT-PCR).The correlations between the levels of miR-142-3 p,miR-150 and thyroid function parameters,related cytokines in HF patients were analyzed.ResultsThe expression levels of miR-142-3 p[(1.54±0.32),(2.25±0.54)]vs.[(1.00±0.23),(1.00±0.24)]and miR-150[(2.21±0.43),(3.78±1.14)]vs.[(1.00±0.16),(1.00±0.19)]in thyroid tis-sue and peripheral blood mononuclear cells in HT group were significantly higher than those in control group(P<0.05).There were significant differences in thyroid function parameters and related cytokines between the two groups.The levels of thyroid stimulating hormone(TSH),thyroid peroxidase antibody(TPOAb),interleukin-17(IL-17),interleukin-6(IL-6),interferon-γ(IFN-γ)and transform-ing growth factorβ1(TGF-β1)in HT group were significantly higher than those in control group(P<0.05).The levels of free triiodothy-ronine(FT3)and free thyroxine(FT4)were significantly lower in HT group than those in control group(P<0.05).The levels of miR-142-3 p and miR-150 in thyroid tissue and peripheral blood mononuclear cells of HT patients were positively correlated,and they were also positively correlated with TSH,TPOAb,IL-17,IL-6,IFN-γ,TGF-β1,while negatively correlated with FT3 and FT4(P<0.05).Conclu
作者
王征
张浩
李伟汉
翟晓建
宋春峰
郭满
WANG Zheng;ZHANG Hao;LI Weihan;ZHAI Xiaojian;SONG Chunfeng;GUO Man(Department of Breast and Thyroid Surgery,Nanyang Central Hospital,Nanyang,Henan 473003,China)
出处
《安徽医药》
CAS
2021年第8期1642-1646,共5页
Anhui Medical and Pharmaceutical Journal
