Objective:To To investigate the changes of MicroRNA-134,CREB and p-CREB expression in epileptic rat brains in order to elucidate the molecular mechanisms of epilepsy,providing new ideas for clinical treatment.Methods:...Objective:To To investigate the changes of MicroRNA-134,CREB and p-CREB expression in epileptic rat brains in order to elucidate the molecular mechanisms of epilepsy,providing new ideas for clinical treatment.Methods:Sixty-four Spraque-Dawley(SD)rats were divided into groups randomly,including control group,six hours after seizure group,24-hour group,threeday group,one-week group,two-week group,four-week group,and eight-week group.All groups were placed under a pilocarpine-induced epilepsy model except the control group,and all rats were decapitated in different points of time.Brain specimens were taken for quantitative PCR experiments,immunohistochemistry and Western blot experiments.The results of the epilepsy model groups and the control group were compared.Results:There were no significant differences between the six hours after seizure group,the 24-hour group and the control group about the MicroRNA-134 levels.MicroRNA-134 in the hippocampus tissue of the three-day group significantly reduced compared with the control group;same result was observed with the one-week,two-week,four-week and eight-week groups.The CREB and p-CREB levels in the three-day group's rat hippocampus significantly increased compared with the control group;and the high levels of CREB and p-CREB were constantly maintained in the one-week,two-week,four-week and eight-week groups.Conclusions:The MicroRNA-134 level of the epileptic rat hippocampus is significantly lower than normal after three days,and continues to maintain a low level:while CREB and p-CREB levels are rsignificantly increased after three days,and continue to remain at a high level.MicroRNA-134 plays a role in inhibiting synaptic plasticity by inhibiting CREB and p-CREB expressions.展开更多
目的观察双相躁狂患者血浆中微小RNA-134(micro RNA-134)及LIM激酶-1(LIMK-1)m RNA的表达水平与双相躁狂之间的关系。方法 105例双相躁狂患者作为患者组,100例与患者组在年龄和性别上匹配的健康志愿者作为对照组,患者组常规应用情绪稳...目的观察双相躁狂患者血浆中微小RNA-134(micro RNA-134)及LIM激酶-1(LIMK-1)m RNA的表达水平与双相躁狂之间的关系。方法 105例双相躁狂患者作为患者组,100例与患者组在年龄和性别上匹配的健康志愿者作为对照组,患者组常规应用情绪稳定剂治疗1个月。患者组于入组时和治疗1个月时、对照组于入组时分别评定Bech-Rafaelsen躁狂量表(BRMS)评分表并收集血浆,采用实时荧光定量聚合酶链式反应(PCR)技术检测血浆micro RNA-134及LIMK-1 m RNA的表达水平,最后对检测结果及BRMS评分进行统计分析。结果治疗前,患者组血浆micro RNA-134及LIMK-1 m RNA表达水平分别为(1.17±0.02)、(0.37±0.02)显著低于对照组的(1.46±0.04)、(0.46±0.01),差异有统计学意义(P<0.05);治疗1个月后,患者组血浆micro RNA-134、LIMK-1 m RNA表达水平分别为(1.36±0.07)、(0.52±0.01)较治疗前显著升高,差异有统计学意义(P<0.05)。患者组治疗前及治疗后血浆micro RNA-134表达水平与BRMS评分之间均存在负相关(r=-0.48、-0.39,P<0.05)。结论血浆micro RNA-134、LIMK-1 m RNA的表达水平和双相障碍躁狂发作有关,有可能作为双相躁狂的生物标记物。展开更多
基金supported by National Natural Science Foundation(No:81371439)
文摘Objective:To To investigate the changes of MicroRNA-134,CREB and p-CREB expression in epileptic rat brains in order to elucidate the molecular mechanisms of epilepsy,providing new ideas for clinical treatment.Methods:Sixty-four Spraque-Dawley(SD)rats were divided into groups randomly,including control group,six hours after seizure group,24-hour group,threeday group,one-week group,two-week group,four-week group,and eight-week group.All groups were placed under a pilocarpine-induced epilepsy model except the control group,and all rats were decapitated in different points of time.Brain specimens were taken for quantitative PCR experiments,immunohistochemistry and Western blot experiments.The results of the epilepsy model groups and the control group were compared.Results:There were no significant differences between the six hours after seizure group,the 24-hour group and the control group about the MicroRNA-134 levels.MicroRNA-134 in the hippocampus tissue of the three-day group significantly reduced compared with the control group;same result was observed with the one-week,two-week,four-week and eight-week groups.The CREB and p-CREB levels in the three-day group's rat hippocampus significantly increased compared with the control group;and the high levels of CREB and p-CREB were constantly maintained in the one-week,two-week,four-week and eight-week groups.Conclusions:The MicroRNA-134 level of the epileptic rat hippocampus is significantly lower than normal after three days,and continues to maintain a low level:while CREB and p-CREB levels are rsignificantly increased after three days,and continue to remain at a high level.MicroRNA-134 plays a role in inhibiting synaptic plasticity by inhibiting CREB and p-CREB expressions.
文摘目的观察双相躁狂患者血浆中微小RNA-134(micro RNA-134)及LIM激酶-1(LIMK-1)m RNA的表达水平与双相躁狂之间的关系。方法 105例双相躁狂患者作为患者组,100例与患者组在年龄和性别上匹配的健康志愿者作为对照组,患者组常规应用情绪稳定剂治疗1个月。患者组于入组时和治疗1个月时、对照组于入组时分别评定Bech-Rafaelsen躁狂量表(BRMS)评分表并收集血浆,采用实时荧光定量聚合酶链式反应(PCR)技术检测血浆micro RNA-134及LIMK-1 m RNA的表达水平,最后对检测结果及BRMS评分进行统计分析。结果治疗前,患者组血浆micro RNA-134及LIMK-1 m RNA表达水平分别为(1.17±0.02)、(0.37±0.02)显著低于对照组的(1.46±0.04)、(0.46±0.01),差异有统计学意义(P<0.05);治疗1个月后,患者组血浆micro RNA-134、LIMK-1 m RNA表达水平分别为(1.36±0.07)、(0.52±0.01)较治疗前显著升高,差异有统计学意义(P<0.05)。患者组治疗前及治疗后血浆micro RNA-134表达水平与BRMS评分之间均存在负相关(r=-0.48、-0.39,P<0.05)。结论血浆micro RNA-134、LIMK-1 m RNA的表达水平和双相障碍躁狂发作有关,有可能作为双相躁狂的生物标记物。
