摘要
目的探讨睡眠剥夺（SD）对大鼠海马、下降脑、皮质microRNA-132（mir-132）、microRNA-134（mir．134）表达的影响。方法将20只大鼠分为对照组（CON组）、睡眠剥夺组（sD组）。用改良多平台法建立睡眠剥夺模型，用实时定量聚合酶链反应（Realtime—PCR）方法检测正常睡眠、睡眠剥夺大鼠海马、下丘脑、皮质mir-132、mir-134水平。结果SD组大鼠海马脑区mir-132含量较CON组有所升高，差异有统计学意义（CON51．87±8．13，SD67．25±7．59）（P〈0．01）；sD组大鼠海马脑区mir-134含量较CON组有所下降，差异有统计学意义（CON1．82±0．15，SD1．45±0．12）（P〈0．01）；SD组大鼠皮质、下丘脑脑区mir-132、mir．134含量与CON组相比差异无统计学意义（P〉0．05）．皮质mir-132水平分别为CON1．57±0．10，SD1．48±0．11，下丘脑mir-132水平分别为CON 1．37±0．09，SD1．36±0．11；皮质mir-134水平分别为CON98．26d-5．17，SD100．80±4．15，下丘脑mir-134水平分别为CON97．56±6．28，SD91．01±4．07（P〉0．05）。结论Mir-132、mir-134在睡眠剥夺模型大鼠海马的上升和下降提示这两种miRNAs在睡眠剥夺中可能起到了相反的作用，对这两种miRNAs在海马的含量测定有望为睡眠剥夺及其抗抑郁机制的研究找到新的线索。
Objective To investigate the effects of sleep deprivation on expressions of Mir-132, mir-134 in the different regions of rat brain. Methods All the male SD rats were divided into control group （ normal sleep group） ,sleep deprivation （SD）. The modified muhiple platform method （MMPM） was used to establish sleep deprivation model. Mir-132,mir-134 level was detected by real time PCR. Results Mir-132 were significantly increased in SD groups in hippoeampus compared with the control groups （51.87 ± 8. 13 vs 67.25 ± 7.59 ） （P 〈 0.01 ）. Mir-134 were significantly decreased in SD groups compared with the control groups（ 1.82 ±0.15 vs 1.45±0.12） （P〈 0.01 ）. There were no statistically significant differences in cortex and thalamus（P 〉 0.05 ）. Cortex mir-132 level in SD group and control group was 1.57 ± 0.10,1.48 ±0.11 respectively, and it was 1.37 ± 0.09, 1.36 ±0.11 in thalamus;Cortex mir-134 level in SD group and control group was 98.26 ± 5.17,100.80 ± 4.15 respectively, and it was 97.56 ± 6.28,91.01 ± 4.07 in thalamus. Conclusion The upregulation of mir-132 and downregulation of mir-134 implies that two miRNAs did opposite actions in the processes of sleep deprivation. This findings indicate that hippocampus mir-132 ,mir-134 levels in the SD rat may reflect associated depressive pathophysiological processes.
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2012年第6期515-517,共3页
Chinese Journal of Behavioral Medicine and Brain Science
基金
广东省自然科学基金资助项目（$2011010001320）
广东省医学科研基金资助项目（B2010286）
深圳市科技计划项目（201102111）
