Objective： To investigate the expressions of O6-methyguanine-DNA methytransferase（MGMT） and Survivin in colorectal carcinoma and their clinical significance. Methods： Formalin-fixed, paraffin-embedded specimens fr...Objective： To investigate the expressions of O6-methyguanine-DNA methytransferase（MGMT） and Survivin in colorectal carcinoma and their clinical significance. Methods： Formalin-fixed, paraffin-embedded specimens from polypus and colorectal carcinoma were examined with streptavidin-biotin peroxidase（S-P） immunohistochemical technique for the expressions of MGMT and Survivin. Results： We found that there were significant differences in MGMT and Survivin between polypus and colorectal carcinoma. Expression of MGMT was correlated with ages and lymph node metastasis while Survivin was associated with lymph node metastasis only. Meanwhile, the expression of MGMT was correlated with Survivin （P〈0.01, r=0.65）. But there was no significant difference between male and female and the different depth of infiltration. Conclusion： It is concluded that the abnormal expressions of MGMT and Survivin were associated with the degree of malignancy of colorectal tumor. They possibly could be useful indexes for the primary screening and prognosis of colorectal carcinoma. Examination of them may have an important guiding significance in the chemotherapy strategy.展开更多
目的 明确 5- Aza- cd R对 DNA MTase转录水平的抑制作用。方法 用 5× 1 0 -7M的5- AZa- cd R处理肝癌细胞株 SMMC- 772 1和 H2和 Hep G2 ,就用 RT- PCR技术检测经药物处理前后 2株肝癌细胞 DNA MTase m RNA表达水平。结果 经 5...目的 明确 5- Aza- cd R对 DNA MTase转录水平的抑制作用。方法 用 5× 1 0 -7M的5- AZa- cd R处理肝癌细胞株 SMMC- 772 1和 H2和 Hep G2 ,就用 RT- PCR技术检测经药物处理前后 2株肝癌细胞 DNA MTase m RNA表达水平。结果 经 5- Aza- cd R处理后 ,2株肝癌细胞 DNAMTase m RNA表达量显著下降。结论 5- Aza- cd R能抑制肝细胞 DNA MTase转录 。展开更多
基金a grant from the Department of Sci & Tech of Hebei Province(No.03276117)
文摘Objective： To investigate the expressions of O6-methyguanine-DNA methytransferase（MGMT） and Survivin in colorectal carcinoma and their clinical significance. Methods： Formalin-fixed, paraffin-embedded specimens from polypus and colorectal carcinoma were examined with streptavidin-biotin peroxidase（S-P） immunohistochemical technique for the expressions of MGMT and Survivin. Results： We found that there were significant differences in MGMT and Survivin between polypus and colorectal carcinoma. Expression of MGMT was correlated with ages and lymph node metastasis while Survivin was associated with lymph node metastasis only. Meanwhile, the expression of MGMT was correlated with Survivin （P〈0.01, r=0.65）. But there was no significant difference between male and female and the different depth of infiltration. Conclusion： It is concluded that the abnormal expressions of MGMT and Survivin were associated with the degree of malignancy of colorectal tumor. They possibly could be useful indexes for the primary screening and prognosis of colorectal carcinoma. Examination of them may have an important guiding significance in the chemotherapy strategy.
文摘目的 明确 5- Aza- cd R对 DNA MTase转录水平的抑制作用。方法 用 5× 1 0 -7M的5- AZa- cd R处理肝癌细胞株 SMMC- 772 1和 H2和 Hep G2 ,就用 RT- PCR技术检测经药物处理前后 2株肝癌细胞 DNA MTase m RNA表达水平。结果 经 5- Aza- cd R处理后 ,2株肝癌细胞 DNAMTase m RNA表达量显著下降。结论 5- Aza- cd R能抑制肝细胞 DNA MTase转录 。
